Plants having increased tolerance to herbicides

ABSTRACT

The present invention refers to a plant or plant part comprising a polynucleotide encoding a wildtype or mutated cellulose synthase (CESA) polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, such as azines.

This application is a continuation of U.S. patent application Ser. No. 15/305,931, which is the U.S. National Stage application of International Application No. PCT/EP2015/058633, filed Apr. 22, 2015, which claims the benefit of U.S. Patent Application Nos. 61/982,893, 61/982,894, 61/982,895, 61/982,896, 61/982,897, 61/982,898, 61/982,899, 61/982,900, 61/982,901, 61/982,903, and 61/982,904, which were all filed on Apr. 23, 2014; the contents of aforementioned applications are incorporated herein by reference in their entirety.

INCORPORATION BY REFERENCE OF MATERIAL SUBMITTED ELECTRONICALLY

This application incorporates by reference in its entirety a computer-readable nucleotide/amino acid sequence listing identified as one 635,402-byte ASCII (text) file named “76908A_Seqlisting.txt,” created Mar. 19, 2020.

FIELD OF THE INVENTION

The present invention relates in general to methods for conferring on plants agricultural level tolerance to herbicides. Particularly, the invention refers to plants having an increased tolerance to herbicides, more specifically to herbicides which inhibit the enzyme cellulose synthase (CESA), also known cellulose biosynthesis inhibitors (CBI). More specifically, the present invention relates to methods and plants obtained by mutagenesis and cross-breeding and transformation that have an increased tolerance to herbicides, particularly CESA-inhibiting herbicides.

BACKGROUND OF THE INVENTION

Plant cell walls are complex structures composed of high-molecular-weight polysaccharides, proteins, and lignins. Among the wall polysaccharides, cellulose, a hydrogen-bonded/β-1,4-linked glucan microfibril, is the main load-bearing wall component and a key precursor for industrial applications. Cellulose is synthesized by large multimeric cellulose synthase (CESA) complexes (E.C.2.4.1.12), tracking along cortical microtubules at the plasma membrane. The only known components of these complexes are the cellulose synthase proteins. Recent studies have identified tentative interaction partners for the CESAs and shown that the migratory patterns of the CESA complexes depend on phosphorylation status (for review see Endler and Persson, Molecular Plant, 2011, Volume 4, Number 2, Pages 199-211, and references contained therein). For example, cotton cellulose synthase genes, termed CESA1 and CESA2, were identified in a collection of expressed sequence tag (EST) sequences on the basis of weak sequence similarity to genes for cellulose synthase from bacteria (Richmond and Somerville. Plant Physiology, 2000, Vol. 124, 495-498; and references contained therein) In addition, the genes were expressed at high levels in cotton fibers at the onset of secondary wall synthesis and a purified fragment of one of the corresponding proteins as shown to bind UDP-Glc, the proposed substrate for cellulose biosynthesis. The conclusion that the cotton CESA genes are cellulose synthases is supported by results obtained with two cellulose-deficient Arabidopsis mutants, rsw1 and irx3 (Richmond and Somerville. Plant Physiology, Vol. 124, 2000, 495-498; and references contained therein). The genes corresponding to the RSW1 and IRX3 loci exhibit a high degree of sequence similarity to the cotton CESA genes and are considered orthologs. Ten full-length CESA genes have been sequenced from Arabidopsis, and there is a genome survey sequence that may indicate one additional family member. Reiterative database searches using the Arabidopsis Rsw1 (AtCESA1) and the cotton CESA polypeptide sequences as the initial query sequences revealed a large superfamily of at least 41 CESA-like genes in Arabidopsis. Based on predicted protein sequences, these genes were grouped into seven clearly distinguishable families (Richmond and Somerville. Plant Physiology, Vol. 124, 2000, 495-498; and references contained therein): the CESA family, which includes RSW1 and IRX3 (AtCESA7), and six families of structurally related genes of unknown function designated as the “cellulose synthase-like” genes (CsIA, CsIB, CsIC, CsID, CsIE, and CsIG).

WO 2013/142968 describes plant cellulose synthase (CESA) alleles identified by mutagenizing plants and screening said plants with a cellulose biosynthetic inhibitor (CBI). CBIs employed in WO 2013/142968 include dichlobenil, chlorthiamid, isoxaben, flupoxam, and quinclorac, particularly isoxaben or flupoxam (named fpx1-1 to fxp1-3 [CESA3], fxp2-1 to fxp2-3 [CESA1] and ixr1-1 to ixr1-7 [CESA3], ixr2-1 to ixr2-2 [CESA6] mutants of Arabidopsis CESA wildtype enzymes)

The inventors of the present invention have now surprisingly found that over-expression of the mutant cellulose synthase forms disclosed in WO 2013/142968 confers in plants tolerance/resistance to particular classes of CESA-inhibiting herbicides (cellulose biosynthesis inhibitors; CBIS) as compared to the non-transformed and/or non-mutagenized plants or plant cells, respectively. More specifically, the inventors of the present invention have found that CESA expression confers tolerance/resistance to azines. More specifically, the inventors of the present invention have found that modifications of the C-terminal part of CESA proteins confer tolerance/resistance to azines.

The problem of the present invention can be seen as to the provision of novel traits by identifying target polypeptides, the manipulation of which makes plants tolerant to herbicides.

Three main strategies are available for making plants tolerant to herbicides, i.e. (1) detoxifying the herbicide with an enzyme which transforms the herbicide, or its active metabolite, into non-toxic products, such as, for example, the enzymes for tolerance to bromoxynil or to basta (EP242236, EP337899); (2) mutating the target enzyme into a functional enzyme which is less sensitive to the herbicide, or to its active metabolite, such as, for example, the enzymes for tolerance to glyphosate (EP293356, Padgette S. R. et al., J. Biol. Chem., 266, 33, 1991); or (3) overexpressing the sensitive enzyme so as to produce quantities of the target enzyme in the plant which are sufficient in relation to the herbicide, in view of the kinetic constants of this enzyme, so as to have enough of the functional enzyme available despite the presence of its inhibitor.

The problem is solved by the subject-matter of the present invention.

SUMMARY OF THE INVENTION

Accordingly, in one aspect, the present invention provides a plant or plant part comprising a polynucleotide encoding a wildtype or mutated CESA polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides.

In some aspects, the present invention provides a seed capable of germination into a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

In one aspect, the present invention provides a plant cell of or capable of regenerating a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides, wherein the plant cell comprises the polynucleotide operably linked to a promoter.

In another aspect, the present invention provides a plant cell comprising a polynucleotide operably linked to a promoter operable in a cell, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

In other aspects, the present invention provides a plant product prepared from a plant or plant part comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

In some aspects, the present invention provides a progeny or descendant plant derived from a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, wherein the progeny or descendant plant comprises in at least some of its cells the recombinant polynucleotide operably linked to the promoter, the expression of the wildtype or mutated CESA polypeptide conferring to the progeny or descendant plant tolerance to the CESA-inhibiting herbicides.

In other aspects, the present invention provides a method for controlling weeds at a locus for growth of a plant, the method comprising: (a) applying an herbicide composition comprising CESA-inhibiting herbicides to the locus; and (b) planting a seed at the locus, wherein the seed is capable of producing a plant that comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

In some aspects, the present invention provides a method for controlling weeds at a locus for growth of a plant, the method comprising: applying an herbicidal composition comprising CESA-inhibiting herbicides to the locus; wherein said locus is: (a) a locus that contains: a plant or a seed capable of producing said plant; or (b) a locus that is to be after said applying is made to contain the plant or the seed; wherein the plant or the seed comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

In one aspect, step (a) occurs before, after, or concurrently with step (b).

In other aspects, the present invention provides a method of producing a plant having tolerance to CESA-inhibiting herbicides, the method comprising regenerating a plant from a plant cell transformed with a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

In one aspect, the present invention provides a method of producing a progeny plant having tolerance to CESA-inhibiting herbicides, the method comprising: crossing a first CESA-inhibiting herbicides-tolerant plant with a second plant to produce a CESA-inhibiting herbicides-tolerant progeny plant, wherein the first plant and the progeny plant comprise in at least some of their cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

In addition, the present invention refers to a method for identifying a CESA-inhibiting herbicide by using a wildtype or mutated CESA of the present invention encoded by a nucleic acid which comprises the nucleotide sequence of SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a variant, homologue, paralogue or orthologue thereof.

Said method comprises the steps of:

-   a) generating a transgenic cell or plant comprising a nucleic acid     encoding a wildtype or mutated CESA of the present invention,     wherein the wildtype or mutated CESA of the present invention is     expressed; -   b) applying a CESA-inhibiting herbicide to the transgenic cell or     plant of a) and to a control cell or plant of the same variety; -   c) determining the growth or the viability of the transgenic cell or     plant and the control cell or plant after application of said test     compound, and -   d) selecting test compounds which confer reduced growth to the     control cell or plant as compared to the growth of the transgenic     cell or plant.

Another object refers to a method of identifying a nucleotide sequence encoding a wildtype or mutated CESA which is resistant or tolerant to a CESA-inhibiting herbicide, the method comprising:

-   a) generating a library of wildtype or mutated CESA-encoding nucleic     acids, -   b) screening a population of the resulting wildtype or mutated     CESA-encoding nucleic acids by expressing each of said nucleic acids     in a cell or plant and treating said cell or plant with a     CESA-inhibiting herbicide, -   c) comparing the CESA-inhibiting herbicide-tolerance levels provided     by said population of wildtype or mutated CESA encoding nucleic     acids with the CESA-inhibiting herbicide-tolerance level provided by     a control CESA-encoding nucleic acid, -   d) selecting at least one wildtype or mutated CESA-encoding nucleic     acid that provides a significantly increased level of tolerance to a     CESA-inhibiting herbicide as compared to that provided by the     control CESA-encoding nucleic acid.

In a preferred embodiment, the wildtype or mutated CESA-encoding nucleic acid selected in step d) provides at least 2-fold as much tolerance to a CESA-inhibiting herbicide as compared to that provided by the control CESA-encoding nucleic acid.

The resistance or tolerance can be determined by generating a transgenic plant comprising a nucleic acid sequence of the library of step a) and comparing said transgenic plant with a control plant.

Another object refers to a method of identifying a plant or algae containing a nucleic acid encoding a wildtype or mutated CESA which is resistant or tolerant to a CESA-inhibiting herbicide, the method comprising:

-   a) identifying an effective amount of a CESA-inhibiting herbicide in     a culture of plant cells or green algae. -   b) treating said plant cells or green algae with a mutagenizing     agent, -   c) contacting said mutagenized cells population with an effective     amount of CESA-inhibiting herbicide, identified in a), -   d) selecting at least one cell surviving these test conditions, -   e) PCR-amplification and sequencing of CESA genes from cells     selected in d) and comparing such sequences to wild-type CESA gene     sequences, respectively.

In a preferred embodiment, the mutagenizing agent is ethylmethanesulfonate.

Another object refers to an isolated recombinantly produced, and/or chemically synthesized nucleic acid encoding a wildtype or mutated CESA, the nucleic acid comprising the sequence of SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a variant thereof, as defined hereinafter.

In a preferred embodiment, the nucleic acid being identifiable by a method as defined above.

Another object refers to an isolated, recombinantly produced, and/or chemically synthesized wildtype or mutated CESA polypeptide, the polypeptide comprising the sequence set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, a variant, derivative, orthologue, paralogue or homologue thereof, as defined hereinafter.

In still further aspects, the present invention provides a plant or plant part comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait.

In another embodiment, the invention refers to a plant cell transformed by and expressing a a wildtype or mutated CESA nucleic acid according to the present invention or a plant which has been mutated to obtain a plant expressing, preferably over-expressing a wildtype or mutated CESA nucleic acid according to the present invention, wherein expression of said nucleic acid in the plant cell results in increased resistance or tolerance to a CESA-inhibiting herbicide as compared to a wild type variety of the plant cell

In another embodiment, the invention refers to a plant comprising a plant cell according to the present invention, wherein expression of the nucleic acid in the plant results in the plant's increased resistance to CESA-inhibiting herbicide as compared to a wild type variety of the plant.

The plants of the present invention can be transgenic or non-transgenic.

Preferably, the expression of the nucleic acid of the invention in the plant results in the plant's increased resistance to CESA-inhibiting herbicides as compared to a wild type variety of the plant. In another embodiment, the invention refers to a seed produced by a transgenic plant comprising a plant cell of the present invention, wherein the seed is true breeding for an increased resistance to a CESA-inhibiting herbicide as compared to a wild type variety of the seed.

In another embodiment, the invention refers to a method of producing a transgenic plant cell with an increased resistance to a CESA-inhibiting herbicide as compared to a wild type variety of the plant cell comprising, transforming the plant cell with an expression cassette comprising a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide.

In another embodiment, the invention refers to a method of producing a transgenic plant comprising, (a) transforming a plant cell with an expression cassette comprising a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, and (b) generating a plant with an increased resistance to CESA-inhibiting herbicide from the plant cell.

Preferably, the expression cassette further comprises a transcription initiation regulatory region and a translation initiation regulatory region that are functional in the plant.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 A, B, C

FIG. 1 shows phylogenetic trees of cellulose synthase homologues in corn (A), soy (B) and rice (C).

FIG. 2 A, B, C

FIG. 2 shows alignment of all cellulose synthase homologues (A), arabidopsis CESA1, CESA3 and CESA 6 homologues (B) and Arabidopsis CESA1 and CESA3 homologues (C).

KEY TO SEQUENCE LISTING

TABLE 1 SEQ ID NO Amino acid Sequence/Origin  1 At_CESA1  2 At_CESA2  3 At_CESA3  4 At_CESA4  5 At_CESA5  6 At_CESA6  7 At_CESA7  8 At_CESA8  9 At_CESA9 10 At_CESA10 11 GRMZM2G027723|GRMZM2G027723_T01 12 GRMZM2G055795|GRMZM2G055795_T01 13 GRMZM2G039454|GRMZM2G039454_T01 14 GRMZM2G025231|GRMZM2G025231_T02 15 GRMZM2G142898|GRMZM2G142898_T01 16 GRMZM2G424832|GRMZM2G424832_T01 17 GRMZM2G177631|GRMZM2G177631_T01 18 GRMZM2G150404|GRMZM2G150404_T01 19 GRMZM2G028353|GRMZM2G028353_T01 20 GRMZM2G018241|GRMZM2G018241_T01 21 GRMZM2G037413|GRMZM2G037413_T01 22 GRMZM2G082580|GRMZM2G082580_T01 23 GRMZM2G112336|GRMZM2G112336_T01 24 GRMZM2G111642|GRMZM2G111642_T01 25 GRMZM2G113137|GRMZM2G113137_T01 26 Glyma02g36720|Glyma02g36720.1 27 Glyma02g08920|Glyma02g08920.1 28 Glyma12g36570|Glyma12g36570.1 29 Glyma12g17730|Glyma12g17730.1 30 Glyma06g06870|Glyma06g06870.1 31 Glyma06g30860|Glyma06g30860.1 32 Glyma06g47420|Glyma06g47420.1 33 Glyma06g30850|Glyma06g30850.1 34 Glyma06g07320|Glyma06g07320.1 35 Glyma04g07220|Glyma04g07220.1 36 Glyma04g06780|Glyma04g06780.1 37 Glyma04g23530|Glyma04g23530.2 38 Glyma08g15380|Glyma08g15380.1 39 Glyma08g12400|Glyma08g12400.1 40 Glyma17g08000|Glyma17g08000.1 41 Glyma13g27250|Glyma13g27250.3 42 Glyma05g29240|Glyma05g29240.2 43 Glyma05g32100|Glyma05g32100.1 44 Glyma15g43040|Glyma15g43040.1 45 Glyma09g15620|Glyma09g15620.2 46 Glyma10g36790|Glyma10g36790.1 47 Glyma16g28080|Glyma16g28080.2 48 Os01g54620.1 49 Os03g59340.1 50 Os03g62090.1 51 Os05g08370.1 52 Os06g02180.1 53 Os06g22980.1 54 Os06g39970.1 55 Os07g10770.1 56 Os07g14850.1 57 Os07g24190.1 58 Os07g24190.2 59 Os07g24190.3 60 Os08g25710.1 61 Os09g25490.1 62 Os10g32980.1 63 Os10g42750.1 64 Os12g36890.1

DETAILED DESCRIPTION

The articles “a” and “an” are used herein to refer to one or more than one (i.e., to at least one) of the grammatical object of the article. By way of example, “an element” means one or more elements.

As used herein, the word “comprising,” or variations such as “comprises” or “comprising,” will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

The term “control of undesired vegetation or weeds” is to be understood as meaning the killing of weeds and/or otherwise retarding or inhibiting the normal growth of the weeds. Weeds, in the broadest sense, are understood as meaning all those plants which grow in locations where they are undesired. The weeds of the present invention include, for example, dicotyledonous and monocotyledonous weeds. Dicotyledonous weeds include, but are not limited to, weeds of the genera: Sinapis, Lepidium, Galium, Stellaria, Matricaria, Anthemis, Galinsoga, Chenopodium, Urtica, Senecio, Amaranthus, Portulaca, Xanthium, Convolvulus, Ipomoea, Polygonum, Sesbania, Ambrosia, Cirsium, Carduus, Sonchus, Solanum, Rorippa, Rotala, Lindernia, Lamium, Veronica, Abutilon, Emex, Datura, Viola, Galeopsis, Papaver, Centaurea, Trifolium, Ranunculus, and Taraxacum. Monocotyledonous weeds include, but are not limited to, weeds of the genera: Echinochloa, Setaria, Panicum, Digitaria, Phleum, Poa, Festuca, Eleusine, Brachiaria, Lolium, Bromus, Avena, Cyperus, Sorghum, Agropyron, Cynodon, Monochoria, Fimbristyslis, Sagittaria, Eleocharis, Scirpus, Paspalum, Ischaemum, Sphenoclea, Dactyloctenium, Agrostis, Alopecurus, and Apera. In addition, the weeds of the present invention can include, for example, crop plants that are growing in an undesired location. For example, a volunteer maize plant that is in a field that predominantly comprises soybean plants can be considered a weed, if the maize plant is undesired in the field of soybean plants.

The term “plant” is used in its broadest sense as it pertains to organic material and is intended to encompass eukaryotic organisms that are members of the Kingdom Plantae, examples of which include but are not limited to vascular plants, vegetables, grains, flowers, trees, herbs, bushes, grasses, vines, ferns, mosses, fungi and algae, etc, as well as clones, offsets, and parts of plants used for asexual propagation (e.g. cuttings, pipings, shoots, rhizomes, underground stems, clumps, crowns, bulbs, corms, tubers, rhizomes, plants/tissues produced in tissue culture, etc.). The term “plant” further encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, shoots, stems, leaves, roots (including tubers), flowers, florets, fruits, pedicles, peduncles, stamen, anther, stigma, style, ovary, petal, sepal, carpel, root tip, root cap, root hair, leaf hair, seed hair, pollen grain, microspore, cotyledon, hypocotyl, epicotyl, xylem, phloem, parenchyma, endosperm, a companion cell, a guard cell, and any other known organs, tissues, and cells of a plant, and tissues and organs, wherein each of the aforementioned comprise the gene/nucleic acid of interest. The term “plant” also encompasses plant cells, suspension cultures, callus tissue, embryos, meristematic regions, gametophytes, sporophytes, pollen and microspores, again wherein each of the aforementioned comprises the gene/nucleic acid of interest.

Plants that are particularly useful in the methods of the invention include all plants which belong to the superfamily Viridiplantae, in particular monocotyledonous and dicotyledonous plants including fodder or forage legumes, ornamental plants, food crops, trees or shrubs selected from the list comprising Acer spp., Actinidia spp., Abelmoschus spp., Agave sisalana, Agropyron spp., Agrostis stolonifera, Allium spp., Amaranthus spp., Ammophila arenaria, Ananas comosus, Annona spp., Apium graveolens, Arachis spp, Artocarpus spp., Asparagus officinalis, Avena spp. (e.g. Avena sativa, Avena fatua, Avena byzantina, Avena fatua var. sativa, Avena hybrida), Averrhoa carambola, Bambusa sp., Benincasa hispida, Bertholletia excelsea, Beta vulgaris, Brassica spp. (e.g. Brassica napus, Brassica rapa ssp. [canola, oilseed rape, turnip rape]), Cadaba farinosa, Camellia sinensis, Canna indica, Cannabis sativa, Capsicum spp., Carex elata, Carica papaya, Carissa macrocarpa, Carya spp., Carthamus tinctorius, Castanea spp., Ceiba pentandra, Cichorium endivia, Cinnamomum spp., Citrullus lanatus, Citrus spp., Cocos spp., Coffea spp., Colocasia esculenta, Cola spp., Corchorus sp., Coriandrum sativum, Corylus spp., Crataegus spp., Crocus sativus, Cucurbita spp., Cucumis spp., Cynara spp., Daucus carota, Desmodium spp., Dimocarpus longan, Dioscorea spp., Diospyros spp., Echinochloa spp., Elaeis (e.g. Elaeis guineensis, Elaeis oleifera), Eleusine coracana, Eragrostis tef, Erianthus sp., Eriobotrya japonica, Eucalyptus sp., Eugenia uniflora, Fagopyrum spp., Fagus spp., Festuca arundinacea, Ficus carica, Fortunella spp., Fragaria spp., Ginkgo biloba, Glycine spp. (e.g. Glycine max, Soja hispida or Soja max), Gossypium hirsutum, Helianthus spp. (e.g. Helianthus annuus), Hemerocallis fulva, Hibiscus spp., Hordeum spp. (e.g. Hordeum vulgare), Ipomoea batatas, Juglans spp., Lactuca sativa, Lathyrus spp., Lens culinaris, Linum usitatissimum, Litchi chinensis, Lotus spp., Luffa acutangula, Lupinus spp., Luzula sylvatica, Lycopersicon spp. (e.g. Lycopersicon esculentum, Lycopersicon lycopersicum, Lycopersicon pyriforme), Macrotyloma spp., Malus spp., Malpighia emarginata, Mammea americana, Mangifera indica, Manihot spp., Manilkara zapota, Medicago sativa, Melilotus spp., Mentha spp., Miscanthus sinensis, Momordica spp., Morus nigra, Musa spp., Nicotiana spp., Olea spp., Opuntia spp., Ornithopus spp., Oryza spp. (e.g. Oryza sativa, Oryza latifolia), Panicum miliaceum, Panicum virgatum, Passiflora edulis, Pastinaca sativa, Pennisetum sp., Persea spp., Petroselinum crispum, Phalaris arundinacea, Phaseolus spp., Phleum pratense, Phoenix spp., Phragmites australis, Physalis spp., Pinus spp., Pistacia vera, Pisum spp., Poa spp., Populus spp., Prosopis spp., Prunus spp., Psidium spp., Punica granatum, Pyrus communis, Quercus spp., Raphanus sativus, Rheum rhabarbarum, Ribes spp., Ricinus communis, Rubus spp., Saccharum spp., Salix sp., Sambucus spp., Secale cereale, Sesamum spp., Sinapis sp., Solanum spp. (e.g. Solanum tuberosum, Solanum integrifolium or Solanum lycopersicum), Sorghum bicolor, Spinacia spp., Syzygium spp., Tagetes spp., Tamarindus indica, Theobroma cacao, Trifolium spp., Tripsacum dactyloides, Triticosecale rimpaui, Triticum spp. (e.g. Triticum aestivum, Triticum durum, Triticum turgidum, Triticum hybernum, Triticum macha, Triticum sativum, Triticum monococcum or Triticum vulgare), Tropaeolum minus, Tropaeolum majus, Vaccinium spp., Vicia spp., Vigna spp., Viola odorata, Vitis spp., Zea mays, Zizania palustris, Ziziphus spp., amaranth, artichoke, asparagus, broccoli, Brussels sprouts, cabbage, canola, carrot, cauliflower, celery, collard greens, flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean, strawberry, sugar beet, sugar cane, sunflower, tomato, squash, tea and algae, amongst others. According to a preferred embodiment of the present invention, the plant is a crop plant. Examples of crop plants include inter alia soybean, sunflower, canola, alfalfa, rapeseed, cotton, tomato, potato or tobacco. Further preferably, the plant is a monocotyledonous plant, such as sugarcane. Further preferably, the plant is a cereal, such as rice, maize, wheat, barley, millet, rye, sorghum or oats.

Generally, the term “herbicide” is used herein to mean an active ingredient that kills, controls or otherwise adversely modifies the growth of plants. The preferred amount or concentration of the herbicide is an “effective amount” or “effective concentration.” By “effective amount” and “effective concentration” is intended an amount and concentration, respectively, that is sufficient to kill or inhibit the growth of a similar, wild-type, plant, plant tissue, plant cell, or host cell, but that said amount does not kill or inhibit as severely the growth of the herbicide-resistant plants, plant tissues, plant cells, and host cells of the present invention. Typically, the effective amount of a herbicide is an amount that is routinely used in agricultural production systems to kill weeds of interest. Such an amount is known to those of ordinary skill in the art. Herbicidal activity is exhibited by herbicides useful for the present invention when they are applied directly to the plant or to the locus of the plant at any stage of growth or before planting or emergence. The effect observed depends upon the plant species to be controlled, the stage of growth of the plant, the application parameters of dilution and spray drop size, the particle size of solid components, the environmental conditions at the time of use, the specific compound employed, the specific adjuvants and carriers employed, the soil type, and the like, as well as the amount of chemical applied. These and other factors can be adjusted as is known in the art to promote non-selective or selective herbicidal action. Generally, it is preferred to apply the herbicide postemergence to relatively immature undesirable vegetation to achieve the maximum control of weeds.

By a “herbicide-tolerant” or “herbicide-resistant” plant, it is intended that a plant that is tolerant or resistant to at least one herbicide at a level that would normally kill, or inhibit the growth of, a normal or wild-type plant. Levels of herbicide that normally inhibit growth of a non-tolerant plant are known and readily determined by those skilled in the art. Examples include the amounts recommended by manufacturers for application. The maximum rate is an example of an amount of herbicide that would normally inhibit growth of a non-tolerant plant. For the present invention, the terms “herbicide-tolerant” and “herbicide-resistant” are used interchangeably and are intended to have an equivalent meaning and an equivalent scope. Similarly, the terms “herbicide-tolerance” and “herbicide-resistance” are used interchangeably and are intended to have an equivalent meaning and an equivalent scope. Similarly, the terms “tolerant” and “resistant” are used interchangeably and are intended to have an equivalent meaning and an equivalent scope. As used herein, in regard to an herbicidal composition useful in various embodiments hereof, terms such as CESA-inhibiting herbicides, and the like, refer to those agronomically acceptable herbicide active ingredients (A.I.) recognized in the art. Similarly, terms such as fungicide, nematicide, pesticide, and the like, refer to other agronomically acceptable active ingredients recognized in the art.

When used in reference to a particular mutant enzyme or polypeptide, terms such as herbicide-tolerant and herbicide-tolerance refer to the ability of such enzyme or polypeptide to perform its physiological activity in the presence of an amount of an herbicide A.I. that would normally inactivate or inhibit the activity of the wild-type (non-mutant) version of said enzyme or polypeptide. For example, when used specifically in regard to a CESA enzyme, it refers specifically to the ability to tolerate a CESA-inhibitor. By “herbicide-tolerant wildtype or mutated CESA protein” or “herbicide-resistant wildtype or mutated CESA protein”, it is intended that such a CESA protein displays higher CESA activity, relative to the CESA activity of a wild-type CESA protein, when in the presence of at least one herbicide that is known to interfere with CESA activity and at a concentration or level of the herbicide that is known to inhibit the CESA activity of the wild-type CESA protein. Furthermore, the CESA activity of such a herbicide-tolerant or herbicide-resistant wildtype or mutated CESA protein may be referred to herein as “herbicide-tolerant” or “herbicide-resistant” CESA activity.

As used herein, “recombinant,” when referring to nucleic acid or polypeptide, indicates that such material has been altered as a result of human application of a recombinant technique, such as by polynucleotide restriction and ligation, by polynucleotide overlap-extension, or by genomic insertion or transformation. A gene sequence open reading frame is recombinant if that nucleotide sequence has been removed from it natural text and cloned into any type of artificial nucleic acid vector. The term recombinant also can refer to an organism having a recombinant material, e.g., a plant that comprises a recombinant nucleic acid can be considered a recombinant plant.

The term “transgenic plant” refers to a plant that comprises a heterologous polynucleotide. Preferably, the heterologous polynucleotide is stably integrated within the genome such that the polynucleotide is passed on to successive generations. The heterologous polynucleotide may be integrated into the genome alone or as part of a recombinant expression cassette. “Transgenic” is used herein to refer to any cell, cell line, callus, tissue, plant part or plant, the genotype of which has been so altered by the presence of heterologous nucleic acid including those transgenic organisms or cells initially so altered, as well as those created by crosses or asexual propagation from the initial transgenic organism or cell. In some embodiments, a “recombinant” organism is a “transgenic” organism. The term “transgenic” as used herein is not intended to encompass the alteration of the genome (chromosomal or extra-chromosomal) by conventional plant breeding methods (e.g., crosses) or by naturally occurring events such as, e.g., self-fertilization, random cross-fertilization, non-recombinant viral infection, non-recombinant bacterial transformation, non-recombinant transposition, or spontaneous mutation.

As used herein, “mutagenized” refers to an organism or DNA thereof having alteration(s) in the biomolecular sequence of its native genetic material as compared to the sequence of the genetic material of a corresponding wild-type organism or DNA, wherein the alteration(s) in genetic material were induced and/or selected by human action. Examples of human action that can be used to produce a mutagenized organism or DNA include, but are not limited to, as illustrated in regard to herbicide tolerance: tissue culture of plant cells (e.g., calli) and selection thereof with herbicides (e.g., CESA-inhibiting herbicides), treatment of plant cells with a chemical mutagen such as EMS and subsequent selection with herbicide(s); or by treatment of plant cells with x-rays and subsequent selection with herbicide(s). Any method known in the art can be used to induce mutations. Methods of inducing mutations can induce mutations in random positions in the genetic material or can induce mutations in specific locations in the genetic material (i.e., can be directed mutagenesis techniques), such as by use of a genoplasty technique.

As used herein, a “genetically modified organism” (GMO) is an organism whose genetic characteristics contain alteration(s) that were produced by human effort causing transfection that results in transformation of a target organism with genetic material from another or “source” organism, or with synthetic or modified-native genetic material, or an organism that is a descendant thereof that retains the inserted genetic material. The source organism can be of a different type of organism (e.g., a GMO plant can contain bacterial genetic material) or from the same type of organism (e.g., a GMO plant can contain genetic material from another plant). As used herein in regard to plants and other organisms, “recombinant,” “transgenic,” and “GMO” are considered synonyms and indicate the presence of genetic material from a different source; in contrast, “mutagenized” is used to refer to a plant or other organism, or the DNA thereof, in which no such transgenic material is present, but in which the native genetic material has become mutated so as to differ from a corresponding wild-type organism or DNA.

As used herein, “wild-type” or “corresponding wild-type plant” means the typical form of an organism or its genetic material, as it normally occurs, as distinguished from, e.g., mutagenized and/or recombinant forms. Similarly, by “control cell” or “similar, wild-type, plant, plant tissue, plant cell or host cell” is intended a plant, plant tissue, plant cell, or host cell, respectively, that lacks the herbicide-resistance characteristics and/or particular polynucleotide of the invention that are disclosed herein. The use of the term “wild-type” is not, therefore, intended to imply that a plant, plant tissue, plant cell, or other host cell lacks recombinant DNA in its genome, and/or does not possess herbicide-resistant characteristics that are different from those disclosed herein.

As used herein, “descendant” refers to any generation plant. In some embodiments, a descendant is a first, second, third, fourth, fifth, sixth, seventh, eight, ninth, or tenth generation plant.

As used herein, “progeny” refers to a first generation plant.

The term “seed” comprises seeds of all types, such as, for example, true seeds, caryopses, achenes, fruits, tubers, seedlings and similar forms. In the context of Brassica and Sinapis species, “seed” refers to true seed(s) unless otherwise specified. For example, the seed can be seed of transgenic plants or plants obtained by traditional breeding methods. Examples of traditional breeding methods can include cross-breeding, selfing, back-crossing, embryo rescue, in-crossing, out-crossing, inbreeding, selection, asexual propagation, and other traditional techniques as are known in the art.

Although exemplified with reference to specific plants or plant varieties and their hybrids, in various embodiments, the presently described methods using CESA-inhibiting herbicides can be employed with a variety of commercially valuable plants. CESA-inhibiting herbicides-tolerant plant lines described as useful herein can be employed in weed control methods either directly or indirectly, i. e. either as crops for herbicide treatment or as CESA-inhibiting herbicides-tolerance trait donor lines for development, as by traditional plant breeding, to produce other varietal and/or hybrid crops containing such trait or traits. All such resulting variety or hybrids crops, containing the ancestral CESA-inhibiting herbicides-tolerance trait or traits can be referred to herein as progeny or descendant of the ancestral, CESA-inhibiting herbicides-tolerant line(s). Such resulting plants can be said to retain the “herbicide tolerance characteristic(s)” of the ancestral plant, i.e. meaning that they possess and express the ancestral genetic molecular components responsible for the trait.

In one aspect, the present invention provides a plant or plant part comprising a polynucleotide encoding a wildtype or mutated CESA polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides.

In a preferred embodiment, the plant has been previously produced by a process comprising recombinantly preparing a plant by introducing and over-expressing a wildtype or mutated CESA transgene according to the present invention, as described in greater detail hereinafter.

In another preferred embodiment, the plant has been previously produced by a process comprising in situ mutagenizing plant cells or seeds, to obtain plant cells or plants which express a wildtype or mutated CESA.

In another embodiment, the polynucleotide encoding the wildtype or mutated CESA polypeptide comprises the nucleic acid sequence set forth in SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a variant or derivative thereof.

In other embodiments, the wildtype or mutated CESA polypeptide for use according to the present invention is a functional variant having, over the full-length of the variant, at least about 80%, illustratively, at least about 80%, 90%, 95%, 98%, 99% or more amino acid sequence identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64.

In another embodiment, the wildtype or mutated CESA polypeptide for use according to the present invention is a functional fragment of a polypeptide having the amino acid sequence set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64.

It is recognized that the CESA polynucleotide molecules and CESA polypeptides of the invention encompass polynucleotide molecules and polypeptides comprising a nucleotide or an amino acid sequence that is sufficiently identical to nucleotide sequences set forth in SEQ ID Nos: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or to the amino acid sequences set forth in SEQ ID Nos: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64. The term “sufficiently identical” is used herein to refer to a first amino acid or nucleotide sequence that contains a sufficient or minimum number of identical or equivalent (e.g., with a similar side chain) amino acid residues or nucleotides to a second amino acid or nucleotide sequence such that the first and second amino acid or nucleotide sequences have a common structural domain and/or common functional activity.

Generally, “sequence identity” refers to the extent to which two optimally aligned DNA or amino acid sequences are invariant throughout a window of alignment of components, e.g., nucleotides or amino acids. An “identity fraction” for aligned segments of a test sequence and a reference sequence is the number of identical components that are shared by the two aligned sequences divided by the total number of components in reference sequence segment, i.e., the entire reference sequence or a smaller defined part of the reference sequence. “Percent identity” is the identity fraction times 100. Optimal alignment of sequences for aligning a comparison window are well known to those skilled in the art and may be conducted by tools such as the local homology algorithm of Smith and Waterman, the homology alignment algorithm of Needleman and Wunsch, the search for similarity method of Pearson and Lipman, and preferably by computerized implementations of these algorithms such as GAP, BESTFIT, FASTA, and TFASTA available as part of the GCG. Wisconsin Package. (Accelrys Inc. Burlington, Mass.)

Polynucleotides and Oligonucleotides

By an “isolated polynucleotide”, including DNA, RNA, or a combination of these, single or double stranded, in the sense or antisense orientation or a combination of both, dsRNA or otherwise, we mean a polynucleotide which is at least partially separated from the polynucleotide sequences with which it is associated or linked in its native state. Preferably, the isolated polynucleotide is at least 60% free, preferably at least 75% free, and most preferably at least 90% free from other components with which they are naturally associated. As the skilled addressee would be aware, an isolated polynucleotide can be an exogenous polynucleotide present in, for example, a transgenic organism which does not naturally comprise the polynucleotide. Furthermore, the terms “polynucleotide(s)”, “nucleic acid sequence(s)”, “nucleotide sequence(s)”, “nucleic acid(s)”, “nucleic acid molecule” are used interchangeably herein and refer to nucleotides, either ribonucleotides or deoxyribonucleotides or a combination of both, in a polymeric unbranched form of any length.

The term “mutated CESA nucleic acid” refers to a CESA nucleic acid having a sequence that is mutated from a wild-type CESA nucleic acid and that confers increased CESA-inhibiting herbicide tolerance to a plant in which it is expressed. Furthermore, the term “mutated cellulose synthase (mutated CESA)” refers to the replacement of an amino acid of the wild-type primary sequences of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, or a variant, a derivative, a homologue, an orthologue, or paralogue thereof, with another amino acid. The expression “mutated amino acid” will be used below to designate the amino acid which is replaced by another amino acid, thereby designating the site of the mutation in the primary sequence of the protein.

In a preferred embodiment, the CESA nucleotide sequence encoding a mutated CESA comprises the sequence of SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a variant or derivative thereof

Furthermore, it will be understood by the person skilled in the art that the CESA nucleotide sequences encompasse homologues, paralogues and orthologues of SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, as defined hereinafter.

The term “variant” with respect to a sequence (e.g., a polypeptide or nucleic acid sequence such as—for example—a transcription regulating nucleotide sequence of the invention) is intended to mean substantially similar sequences. For nucleotide sequences comprising an open reading frame, variants include those sequences that, because of the degeneracy of the genetic code, encode the identical amino acid sequence of the native protein. Naturally occurring allelic variants such as these can be identified with the use of well-known molecular biology techniques, as, for example, with polymerase chain reaction (PCR) and hybridization techniques. Variant nucleotide sequences also include synthetically derived nucleotide sequences, such as those generated, for example, by using site-directed mutagenesis and for open reading frames, encode the native protein comprising the sequence of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, as well as those that encode a polypeptide having amino acid substitutions relative to the native protein, e.g. the mutated CESA according to the present invention as disclosed herein. Generally, nucleotide sequence variants of the invention will have at least 30, 40, 50, 60, to 70%, e.g., preferably 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, to 79%, generally at least 80%, e.g., 81%-84%, at least 85%, e.g., 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, to 98% and 99% nucleotide “sequence identity” to the nucleotide sequence of SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77. The % identity of a polynucleotide is determined by GAP (Needleman and Wunsch, 1970) analysis (GCG program) with a gap creation penalty=5, and a gap extension penalty=0.3. Unless stated otherwise, the query sequence is at least 45 nucleotides in length, and the GAP analysis aligns the two sequences over a region of at least 45 nucleotides. Preferably, the query sequence is at least 150 nucleotides in length, and the GAP analysis aligns the two sequences over a region of at least 150 nucleotides. More preferably, the query sequence is at least 300 nucleotides in length and the GAP analysis aligns the two sequences over a region of at least 300 nucleotides. Even more preferably, the GAP analysis aligns the two sequences over their entire length.

Polypeptides

By “substantially purified polypeptide” or “purified” a polypeptide is meant that has been separated from one or more lipids, nucleic acids, other polypeptides, or other contaminating molecules with which it is associated in its native state. It is preferred that the substantially purified polypeptide is at least 60% free, more preferably at least 75% free, and more preferably at least 90% free from other components with which it is naturally associated. As the skilled addressee will appreciate, the purified polypeptide can be a recombinantly produced polypeptide. The terms “polypeptide” and “protein” are generally used interchangeably and refer to a single polypeptide chain which may or may not be modified by addition of non-amino acid groups. It would be understood that such polypeptide chains may associate with other polypeptides or proteins or other molecules such as co-factors. The terms “proteins” and “polypeptides” as used herein also include variants, mutants, modifications, analogous and/or derivatives of the polypeptides of the invention as described herein.

The % identity of a polypeptide is determined by GAP (Needleman and Wunsch, 1970) analysis (GCG program) with a gap creation penalty=5, and a gap extension penalty=0.3. The query sequence is at least 25 amino acids in length, and the GAP analysis aligns the two sequences over a region of at least 25 amino acids. More preferably, the query sequence is at least 50 amino acids in length, and the GAP analysis aligns the two sequences over a region of at least 50 amino acids. More preferably, the query sequence is at least 100 amino acids in length and the GAP analysis aligns the two sequences over a region of at least 100 amino acids. Even more preferably, the query sequence is at least 250 amino acids in length and the GAP analysis aligns the two sequences over a region of at least 250 amino acids. Even more preferably, the GAP analysis aligns the two sequences over their entire length.

With regard to a defined polypeptide, it will be appreciated that % identity figures higher than those provided above will encompass preferred embodiments. Thus, where applicable, in light of the minimum % identity figures, it is preferred that the CESA polypeptide of the invention comprises an amino acid sequence which is at least 40%, more preferably at least 45%, more preferably at least 50%, more preferably at least 55%, more preferably at least 60%, more preferably at least 65%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 91%, more preferably at least 92%, more preferably at least 93%, more preferably at least 94%, more preferably at least 95%, more preferably at least 96%, more preferably at least 97%, more preferably at least 98%, more preferably at least 99%, more preferably at least 99.1%, more preferably at least 99.2%, more preferably at least 99.3%, more preferably at least 99.4%, more preferably at least 99.5%, more preferably at least 99.6%, more preferably at least 99.7%, more preferably at least 99.8%, and even more preferably at least 99.9% identical to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64.

By “variant” polypeptide is intended a polypeptide derived from the protein of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, by deletion (so-called truncation) or addition of one or more amino acids to the N-terminal and/or C-terminal end of the native protein; deletion or addition of one or more amino acids at one or more sites in the native protein; or substitution of one or more amino acids at one or more sites in the native protein. Such variants may result from, for example, genetic polymorphism or from human manipulation. Methods for such manipulations are generally known in the art.

“Derivatives” of a protein encompass peptides, oligopeptides, polypeptides, proteins and enzymes having amino acid substitutions, deletions and/or insertions relative to the unmodified protein in question and having similar biological and functional activity as the unmodified protein from which they are derived. Thus, functional variants and fragments of the CESA polypeptides, and nucleic acid molecules encoding them, also are within the scope of the present invention, and unless specifically described otherwise, irrespective of the origin of said polypeptide and irrespective of whether it occurs naturally. Various assays for functionality of a CESA polypeptide can be employed. For example, a functional variant or fragment of the CESA polypeptide can be assayed to determine its ability to confer CESA-inhibiting herbicides tolerance. By way of illustration, a CESA-inhibiting herbicides tolerance can be defined as insensitivity to CESA inhibiting herbicides sufficient to provide a determinable increase in tolerance to CESA-inhibiting herbicides in a plant or plant part comprising a recombinant polynucleotide encoding the variant or fragment of the CESA polypeptide, wherein the plant or plant part expresses the variant or fragment at up to about 0.5%, illustratively, about 0.05 to about 0.5%, about 0.1 to about 0.4%, and about 0.2 to about 0.3%, of the total cellular protein relative to a similarly treated control plant that does not express the variant or fragment.

In a preferred embodiment, the mutated CESA polypeptide is a functional variant or fragment of a cellulose synthase having the amino acid sequence set forth in SEQ ID NO: 1 or 3, wherein the functional variant or fragment has at least about 80% amino acid sequence identity to SEQ ID NO:1 or 3.

In other embodiments, the functional variant or fragment further has a CESA-inhibiting herbicides tolerance defined as insensitivity to CESA inhibiting herbicides sufficient to provide a determinable increase in tolerance to CESA-inhibiting herbicides in a plant or plant part comprising a recombinant polynucleotide encoding the variant or fragment, wherein the plant or plant part expresses the variant or fragment at up to about 0.5% of the total cellular protein to a similarly treated control plant that does not express the variant or fragment.

“Homologues” of a protein encompass peptides, oligopeptides, polypeptides, proteins and enzymes having amino acid substitutions, deletions and/or insertions relative to the unmodified protein in question and having similar biological and functional activity as the unmodified protein from which they are derived.

In addition, one of ordinary skill in the art will further appreciate that changes can be introduced by mutation into the nucleotide sequences of the invention thereby leading to changes in the amino acid sequence of the encoded proteins without altering the biological activity of the proteins. Thus, for example, an isolated polynucleotide molecule encoding a mutated CESA polypeptide having an amino acid sequence that differs from that of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, can be created by introducing one or more nucleotide substitutions, additions, or deletions into the corresponding nucleotide sequence, such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein. Mutations can be introduced by standard techniques, such as site-directed mutagenesis and PCR-mediated mutagenesis. Such variant nucleotide sequences are also encompassed by the present invention. For example, preferably, conservative amino acid substitutions may be made at one or more predicted preferably nonessential amino acid residues. A “nonessential” amino acid residue is a residue that can be altered from the wild-type sequence of a protein without altering the biological activity, whereas an “essential” amino acid residue is required for biological activity.

A deletion refers to removal of one or more amino acids from a protein.

An insertion refers to one or more amino acid residues being introduced into a predetermined site in a protein. Insertions may comprise N-terminal and/or C-terminal fusions as well as intra-sequence insertions of single or multiple amino acids. Generally, insertions within the amino acid sequence will be smaller than N- or C-terminal fusions, of the order of about 1 to 10 residues. Examples of N- or C-terminal fusion proteins or peptides include the binding domain or activation domain of a transcriptional activator as used in the yeast two-hybrid system, phage coat proteins, (histidine)-6-tag, glutathione S-transferase-tag, protein A, maltose-binding protein, dihydrofolate reductase, Tag•100 epitope, c-myc epitope, FLAG®-epitope, IacZ, CMP (calmodulin-binding peptide), HA epitope, protein C epitope and VSV epitope.

A substitution refers to replacement of amino acids of the protein with other amino acids having similar properties (such as similar hydrophobicity, hydrophilicity, antigenicity, propensity to form or break α-helical structures or β-sheet structures). Amino acid substitutions are typically of single residues, but may be clustered depending upon functional constraints placed upon the polypeptide and may range from 1 to 10 amino acids; insertions will usually be of the order of about 1 to 10 amino acid residues. A conservative amino acid substitution is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Such substitutions would not be made for conserved amino acid residues, or for amino acid residues residing within a conserved motif. Conservative substitution tables are well known in the art (see for example Creighton (1984) Proteins. W.H. Freeman and Company (Eds).

Amino acid substitutions, deletions and/or insertions may readily be made using peptide synthetic techniques well known in the art, such as solid phase peptide synthesis and the like, or by recombinant DNA manipulation. Methods for the manipulation of DNA sequences to produce substitution, insertion or deletion variants of a protein are well known in the art. For example, techniques for making substitution mutations at predetermined sites in DNA are well known to those skilled in the art and include M13 mutagenesis, T7-Gen in vitro mutagenesis (USB, Cleveland, Ohio), QuickChange Site Directed mutagenesis (Stratagene, San Diego, Calif.), PCR-mediated site-directed mutagenesis or other site-directed mutagenesis protocols.

“Derivatives” further include peptides, oligopeptides, polypeptides which may, compared to the amino acid sequence of the naturally-occurring form of the protein, such as the protein of interest, comprise substitutions of amino acids with non-naturally occurring amino acid residues, or additions of non-naturally occurring amino acid residues. “Derivatives” of a protein also encompass peptides, oligopeptides, polypeptides which comprise naturally occurring altered (glycosylated, acylated, prenylated, phosphorylated, myristoylated, sulphated etc.) or non-naturally altered amino acid residues compared to the amino acid sequence of a naturally-occurring form of the polypeptide. A derivative may also comprise one or more non-amino acid substituents or additions compared to the amino acid sequence from which it is derived, for example a reporter molecule or other ligand, covalently or non-covalently bound to the amino acid sequence, such as a reporter molecule which is bound to facilitate its detection, and non-naturally occurring amino acid residues relative to the amino acid sequence of a naturally-occurring protein. Furthermore, “derivatives” also include fusions of the naturally-occurring form of the protein with tagging peptides such as FLAG, HIS6 or thioredoxin (for a review of tagging peptides, see Terpe, Appl. Microbiol. Biotechnol. 60, 523-533, 2003).

“Orthologues” and “paralogues” encompass evolutionary concepts used to describe the ancestral relationships of genes. Paralogues are genes within the same species that have originated through duplication of an ancestral gene; orthologues are genes from different organisms that have originated through speciation, and are also derived from a common ancestral gene. A non-limiting list of examples of such orthologues is shown in Table 1. It will be understood by the person skilled in the art that the sequences of SEQ ID NOs: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, as listed in Table 1 represent orthologues and paralogues to SEQ ID NO:1.

It is well-known in the art that paralogues and orthologues may share distinct domains harboring suitable amino acid residues at given sites, such as binding pockets for particular substrates or binding motifs for interaction with other proteins.

The term “domain” refers to a set of amino acids conserved at specific positions along an alignment of sequences of evolutionarily related proteins. While amino acids at other positions can vary between homologues, amino acids that are highly conserved at specific positions indicate amino acids that are likely essential in the structure, stability or function of a protein. Identified by their high degree of conservation in aligned sequences of a family of protein homologues, they can be used as identifiers to determine if any polypeptide in question belongs to a previously identified polypeptide family.

The term “motif” or “consensus sequence” refers to a short conserved region in the sequence of evolutionarily related proteins. Motifs are frequently highly conserved parts of domains, but may also include only part of the domain, or be located outside of conserved domain (if all of the amino acids of the motif fall outside of a defined domain).

In a preferred embodiment, the CESA polypeptide useful for the present invention, comprises one or more of the following motifs:

i) Motif 1a: (SEQ ID NO: 78) [V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G] [S/N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[F/M/L]G [K/R][L/V][F/L]F. Preferably said motif is (motif 1b; SEQ ID NO: 79) [V/I]AG[V/I]S[Y/D/N]A[V/I][N/S][S/N]G[Y/F][Q/D] SWGPL[F/M/L]G[K/R]L[F/L]F. More preferably said motif is (motif 1c; SEQ ID NO: 80) VAG[V/I]SYA[V/I]NSGYQSWGPL[F/M]GKL[F/L]F ii) Motif 2a: (SEQ ID NO: 81) [V/L/I]W[S/A][V/A/I]LL[A/S]S[I/F/V][F/L][S/T] [L/V][L/M/V/I]WV[R/K][I/V][N/D]PF Preferably, said motif is (motif 2b; SEQ ID NO: 82) VW[S/A][V/A/I]LL[A/S]S[I/F][F/L][S/T][L/V][L/M] WV[R/K][I/V][N/D]PF; More preferably said motif is (motif 2c; SEQ ID NO: 83) VW[S/A][V/A/I]LLASIFSL[L/M]WV[R/K]I[N/D]PF

Motifs 1a-c, 2a-c, given above were derived using the ClustalW algorithm to generate the alignments of cellulose synthase sequences (FIG. 2 A-C) (Larkin et al., Bioinformatics 23:21 (2007) 2947-2948 pp. 28-36). The motifs were essentially derived based on sequence alignment; highly conserved regions were identified that contain the site of mutations conferring azine-herbicide tolerance. Residues within square brackets represent alternatives.

In a preferred embodiment, a CESA polypeptide as applied herein comprises, at least 1, at least 2, selected from the group comprising motifs 1a, 2a, as given above. Alternatively or in addition, in another preferred embodiment, a CESA polypeptide as applied herein comprises at least 1, at least 2, motifs selected from the group comprising motifs 1b, 2b, as given above. Alternatively or in addition, in another preferred embodiment, a CESA polypeptide as applied herein comprises at least 1, at least 2, motifs selected from the group comprising motifs 1c, 2c, as given above.

Additionally or alternatively, the homologue of a CESA protein has in increasing order of preference at least 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% overall sequence identity to the amino acid represented by SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, provided that the homologous protein comprises any one or more of the conserved motifs 1 and/or 2 as outlined above. The overall sequence identity is determined using a global alignment algorithm, such as the Needleman Wunsch algorithm in the program GAP (GCG Wisconsin Package, Accelrys), preferably with default parameters and preferably with sequences of mature proteins (i.e. without taking into account secretion signals or transit peptides). Compared to overall sequence identity, the sequence identity will generally be higher when only conserved domains or motifs are considered. Preferably the motifs in a CESA polypeptide have, in increasing order of preference, at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to any one or more of the motifs represented by SEQ ID NO: 78, 79, 80, 81, 82, and 83 (Motifs 1a, 1b, 1c, 2a, 2b, 2c).

Specialist databases exist for the identification of domains, for example, SMART (Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864; Letunic et al. (2002) Nucleic Acids Res 30, 242-244), InterPro (Mulder et al., (2003) Nucl. Acids. Res. 31, 315-318), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequences motifs and its function in automatic sequence interpretation. (In) ISMB-94; Proceedings 2nd International Conference on Intelligent Systems for Molecular Biology. Altman R., Brutlag D., Karp P., Lathrop R., Searls D., Eds., pp 53-61, AAAI Press, Menlo Park; Hulo et al., Nucl. Acids. Res. 32:D134-D137, (2004)), or Pfam (Bateman et al., Nucleic Acids Research 30(1): 276-280 (2002)). A set of tools for in silico analysis of protein sequences is available on the ExPASy proteomics server (Swiss Institute of Bioinformatics (Gasteiger et al., ExPASy: the proteomics server for in-depth protein knowledge and analysis, Nucleic Acids Res. 31:3784-3788(2003)). Domains or motifs may also be identified using routine techniques, such as by sequence alignment.

Methods for the alignment of sequences for comparison are well known in the art, such methods include GAP, BESTFIT, BLAST, FASTA and TFASTA. GAP uses the algorithm of Needleman and Wunsch ((1970) J Mol Biol 48: 443-453) to find the global (i.e. spanning the complete sequences) alignment of two sequences that maximizes the number of matches and minimizes the number of gaps. The BLAST algorithm (Altschul et al. (1990) J Mol Biol 215: 403-10) calculates percent sequence identity and performs a statistical analysis of the similarity between the two sequences. The software for performing BLAST analysis is publicly available through the National Centre for Biotechnology Information (NCBI). Homologues may readily be identified using, for example, the ClustalW multiple sequence alignment algorithm (version 1.83), with the default pairwise alignment parameters, and a scoring method in percentage (See FIG. 1). Global percentages of similarity and identity may also be determined using one of the methods available in the MatGAT software package (Campanella et al., BMC Bioinformatics. 2003 Jul. 10; 4:29. MatGAT: an application that generates similarity/identity matrices using protein or DNA sequences). Minor manual editing may be performed to optimise alignment between conserved motifs, as would be apparent to a person skilled in the art. Furthermore, instead of using full-length sequences for the identification of homologues, specific domains may also be used. The sequence identity values may be determined over the entire nucleic acid or amino acid sequence or over selected domains or conserved motif(s), using the programs mentioned above using the default parameters. For local alignments, the Smith-Waterman algorithm is particularly useful (Smith T F, Waterman M S (1981) J. Mol. Biol 147(1); 195-7).

The proteins of the invention may be altered in various ways including amino acid substitutions, deletions, truncations, and insertions. Methods for such manipulations are generally known in the art. For example, amino acid sequence variants can be prepared by mutations in the DNA. Methods for mutagenesis and nucleotide sequence alterations are well known in the art. See, for example, Kunkel (1985) PNAS, 82:488-492; Kunkel et al. (1987) Methods in Enzymol. 154:367-382; U.S. Pat. No. 4,873,192; Walker and Gaastra, eds. (1983) Techniques in Molecular Biology (MacMillan Publishing Company, New York) and the references cited therein. Guidance as to appropriate amino acid substitutions that do not affect biological activity of the protein of interest may be found in the model of Dayhoff et al. (1978) Atlas of Protein Sequence and Structure (Natl. Biomed. Res. Found., Wash., D. C), herein incorporated by reference. Conservative substitutions, such as exchanging one amino acid with another having similar properties, may be preferable.

Alternatively, variant nucleotide sequences can be made by introducing mutations randomly along all or part of a coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened to identify mutants that encode proteins that retain activity. For example, following mutagenesis, the encoded protein can be expressed recombinantly, and the activity of the protein can be determined using standard assay techniques.

The inventors of the present invention have found that by substituting one or more of the key amino acid residues of the CESA enzyme of SEQ ID NO: 1 or 3, e.g. by employing one of the above described methods to mutate the CESA encoding nucleic acids, the tolerance or resistance to particular CESA-inhibiting herbicides, collectively named azines, and described in greated detail herein below, could be remarkably increased Preferred substitutions of mutated CESA are those that increase the herbicide tolerance of the plant, but leave the biological activity of the cellulose synthase activity substantially unaffected.

Accordingly, in another object of the present invention refers to a CESA polypeptide, comprising the sequence of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, a variant, derivative, orthologue, paralogue or homologue thereof, the key amino acid residues of which is substituted by any other amino acid.

It will be understood by the person skilled in the art that amino acids located in a close proximity to the positions of amino acids mentioned below may also be substituted. Thus, in another embodiment the variant of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, a variant, derivative, orthologue, paralogue or homologue thereof comprises a mutated CESA, wherein an amino acid ±3, ±2 or ±1 amino acid positions from a key amino acid is substituted by any other amino acid.

Based on techniques well-known in the art, a highly characteristic sequence pattern can be developed, by means of which further of mutated CESA candidates with the desired activity may be searched.

Searching for further mutated CESA candidates by applying a suitable sequence pattern would also be encompassed by the present invention. It will be understood by a skilled reader that the present sequence pattern is not limited by the exact distances between two adjacent amino acid residues of said pattern. Each of the distances between two neighbours in the above patterns may, for example, vary independently of each other by up to ±10, ±5, ±3, ±2 or ±1 amino acid positions without substantially affecting the desired activity.

Furthermore, by applying the method of site directed mutagenesis, in particular saturation mutagenes (see e.g. Schenk et al., Biospektrum March 2006, pages 277-279) PCR based site-directed mutagenesis (e.g. directed mutagenesis kit, Stratagene, Calif., USA or GeneArt Mutagenesis Service, ThermoFisher Scientific Inc., Massachusetts, USA) or systematic mutagenesis (GeneArt Systematic Mutagenesis Service, ThermoFisher Scientific Inc., Massachusetts, USA), the inventors of the present invention have identified and generated specific amino acid substitutions and combinations thereof, which—when introduced into a plant by transforming and expressing the respective mutated CESA encoding nucleic acid-confer increased herbicide resistance or tolerance to a CESA inhibiting herbicide to said plant.

Thus, in preferred embodiment, the variant or derivative of the CESA polypeptide refers to a mutated CESA polypeptide which comprises one or more of the following motifs:

i) Motif 1a: (SEQ ID NO: 78) [V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G] [S/N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[F/M/L]G [K/R][L/V][F/L]F. Preferably said motif is (motif 1b; SEQ ID NO: 79) [V/I]AG[V/I]S[Y/D/N]A[V/I][N/S][S/N]G[Y/F][Q/D] SWGPL[F/M/L]G[K/R]L[F/L]F. More preferably said motif is (motif 1c; SEQ ID NO: 80) VAG[V/I]SYA[V/I]NSGYQSWGPL[F/M]GKL[F/L]F;

-   -   Wherein the amino acid at position 5, 16, 17, and/or 20 within         said motif is substituted by any other amino acid.

ii) Motif 2a: (SEQ ID NO: 81) [V/L/I]W[S/A][V/A/I]LL[A/S]S[I/F/V][F/L][S/T] [L/V][L/M/V/I]WV[R/K][I/V][N/D]PF Preferably, said motif is (motif 2b; SEQ ID NO: 82) VW[S/A][V/A/I]LL[A/S]S[I/F][F/L][S/T][L/V][L/M] WV[R/K][I/V][N/D]PF; More preferably said motif is (motif 2c; SEQ ID NO: 83) VW[S/A][V/A/I]LLASIFSL[L/M]WV[R/K]I[N/D]PF

-   -   Wherein the amino acid at position 8, and/or 11 within said         motif is substituted by any other amino acid

In a more preferred embodiment, the amino acid corresponding to position 5 of motif 1a, 1b, or 1c is:

Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp;

And/or

the amino acid corresponding to position 16 of motif 1a, 1b, or 1c is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

And/or

the amino acid corresponding to position 17 of motif 1a, 1b, or 1c is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Gly, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

And/or

the amino acid corresponding to position 20 of motif 1a, 1b, or 1c is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

In another more preferred embodiment, the amino acid corresponding to position 8 of motif 2a, 2b, or 2c is:

Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp;

And/or

the amino acid corresponding to position 11 of motif 2a, 2b, or 2c is Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

In a particularly preferred embodiment,

the amino acid corresponding to position 5 of motif 1a, 1b, or 1c is Phe,

And/or

the amino acid corresponding to position 16 of motif 1a, 1b, or 1c is Asp,

And/or

the amino acid corresponding to position 17 of motif 1a, 1b, or 1c is Leu,

And/or

the amino acid corresponding to position 20 of motif 1a, 1b, or 1c is Arg.

In another more preferred embodiment,

the amino acid corresponding to position 8 of motif 2a, 2b, or 2c is Phe,

And/or

the amino acid corresponding to position 11 of motif 2a, 2b, or 2c is Leu,

In another preferred embodiment, the variant or derivative of the CESA polypeptide refers to a CESA polypeptide comprising SEQ ID NO: 1, a orthologue, paralogue, or homologue thereof, wherein the amino acid sequence differs from the wildtype amino acid sequence of a CESA polypeptide at one or more positions corresponding to the following positions of SEQ ID NO:1:

998, 1009, 1010, 1013, 1052, 1055.

Examples of differences at these amino acid positions include, but are not limited to, one or more of the following:

the amino acid at or corresponding to position 998 is other than serine;

the amino acid at or corresponding to position 1009 is other than glycine;

the amino acid at or corresponding to position 1010 is other than proline;

the amino acid at or corresponding to position 1013 is other than glycine,

the amino acid at or corresponding to position 1052 is other than serine,

the amino acid at or corresponding to position 1055 is other than serine,

In some embodiments, the mutated CESA enzyme comprising SEQ ID NO: 1, a orthologue, paralogue, or homologue thereof, comprises one or more of the following:

the amino acid corresponding to position 998 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp;

the amino acid corresponding to position 1009 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

the amino acid corresponding to position 1010 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Gly, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

the amino acid corresponding to position 1013 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

the amino acid corresponding to position 1052 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

the amino acid corresponding to position 1055 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp.

In a preferred embodiment, the amino acid corresponding to position 1009 of SEQ ID NO: 1 is Asp.

In another preferred embodiment, the amino acid corresponding to position 1010 of SEQ ID NO: 1 is Leu.

In another preferred embodiment, the amino acid corresponding to position 1013 of SEQ ID NO: 1 is Arg.

In another preferred embodiment, the amino acid corresponding to position 983 of SEQ ID NO: 3 is Phe.

In another preferred embodiment, the amino acid corresponding to position 1037 of SEQ ID NO: 3 is Phe.

In another preferred embodiment, the amino acid corresponding to position 1040 of SEQ ID NO: 3 is Leu.

It will be within the knowledge of the skilled artisan to identify conserved regions and motifs shared between the homologues, orthologues and paralogues encoded by SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, such as those depicted in Table 1. Having identified such conserved regions that may represent suitable binding motifs, amino acids corresponding to the amino acids listed below in Table 2, can be chosen to be substituted by any other amino acid, for example by conserved amino acids, preferably by the amino acid substitutions described SUPRA using SEQ ID NO:1 as reference.

Table 2 provides an overview of positions in the orthologues and homologues to SEQ ID NO:1, i.e. the corresponding positions in SEQ ID NOs: 1 to 64.

TABLE 2 ID Pos 1 Pos 2 Pos 3 Pos 4 Pos 5 Pos 6 1 S998 G1009 P1010 G1013 S1052 S1055 2 S1001 G1012 P1013 G1016 S1055 T1058 3 S983 G994 P995 G998 S1037 S1040 4 S967 G978 P979 G982 S1021 S1024 5 S987 G998 P999 G1002 S1041 T1044 6 S1002 G1013 P1014 G1017 S1056 T1059 7 S944 G955 P956 G959 S998 S1001 8 S901 G912 P913 G916 S955 S958 9 S1005 G1016 P1017 G1020 S1059 T1062 10 S985 G996 P997 G1000 S1039 S1042 11 S991 G1002 P1003 G1006 S1045 S1048 12 S900 G911 P912 G915 S954 S957 13 S991 G1002 P1003 G1006 S1045 S1048 14 S1003 G1014 P1015 G1018 S1057 S1060 15 S974 G985 P986 G989 S1028 S1031 16 S995 G1006 P1007 G1010 S1049 S1052 17 S1011 G1022 P1023 G1026 S1065 S1068 18 S514 G525 P526 G529 S568 S571 19 S1000 G1011 P1012 G1015 S1054 S1057 20 S997 G1008 P1009 G1012 S1051 S1054 21 S900 G911 P912 G915 S954 S957 22 — — — — — — 23 S992 G1003 P1004 G1007 S1046 S1049 24 S994 G1005 P1006 G1009 S1048 S1051 25 S1006 G1017 P1018 G1021 S1060 S1063 26 S951 G962 P963 G966 S1005 S1008 27 S996 G1007 P1008 G1011 S1050 T1053 28 S997 G1008 P1009 G1012 S1051 S1054 29 T891 G902 A903 G906 S945 S948 30 S891 G902 P903 G906 S945 S948 31 S957 G968 P969 G972 S1011 S1014 32 S884 G895 P896 G899 S938 S941 33 T908 G919 A920 G923 S962 S965 34 S1002 G1013 P1014 G1017 S1056 S1059 35 S1002 G1013 P1014 G1017 S1056 S1059 36 S892 G903 P904 G907 S946 S949 37 S957 G968 P969 G972 S1011 S1014 38 S1013 G1024 P1025 G1028 S1067 T1070 39 S905 G916 P917 G920 S959 S962 40 S951 G962 P963 G966 S1005 S1008 41 S998 G1009 P1010 G1013 S1052 S1055 42 S874 G885 P886 G889 S928 S931 43 S1013 G1024 P1025 G1028 S1067 T1070 44 S991 G1002 P1003 G1006 S1045 S1048 45 S992 G1003 P1004 G1007 S1046 S1049 46 S1013 G1024 P1025 G1028 S1067 S1070 47 S996 G1007 P1008 G1011 S1050 T1053 48 S906 G917 P918 G921 S960 S963 49 S991 G1002 P1003 G1006 S1045 S1048 50 S1009 G1020 P1021 G1024 S1063 S1066 51 S993 G1004 P1005 G1008 S1047 S1050 52 S1091 S1102 K1103 G1106 I1145 S1148 53 S935 S946 K947 G950 I989 S992 54 A778 G790 A791 A794 S834 S837 55 S999 G1010 P1011 G1014 S1053 S1056 56 S1009 G1020 P1021 G1024 S1063 S1066 57 S1010 G1021 P1022 G1025 S1064 S1067 58 S754 G765 P766 G769 S808 S811 59 S883 G894 P895 G898 S937 S940 60 A1033 G1044 K1045 G1048 I1087 S1090 61 S973 G984 P985 G988 S1027 S1030 62 S981 G992 P993 G996 S1035 S1038 63 S1046 S1057 K1058 G1061 I1100 S1103 64 A1134 S1145 K1146 G1149 M1188 S1191

Another object refers to a method of identifying a nucleotide sequence encoding a mutated CESA which is resistant or tolerant to a CESA-inhibiting herbicide, the method comprising:

-   a) generating a library of mutated CESA-encoding nucleic acids, -   b) screening a population of the resulting mutated CESA-encoding     nucleic acids by expressing each of said nucleic acids in a cell or     plant and treating said cell or plant with a CESA-inhibiting     herbicide, -   c) comparing the CESA-inhibiting herbicide-tolerance levels provided     by said population of mutated CESA encoding nucleic acids with the     CESA-inhibiting herbicide-tolerance level provided by a control     CESA-encoding nucleic acid, -   d) selecting at least one mutated CESA-encoding nucleic acid that     provides a significantly increased level of tolerance to a     CESA-inhibiting herbicide as compared to that provided by the     control CESA-encoding nucleic acid.

In a preferred embodiment, the mutated CESA-encoding nucleic acid selected in step d) provides at least 2-fold as much resistance or tolerance of a cell or plant to a CESA-inhibiting herbicide as compared to that provided by the control CESA-encoding nucleic acid.

In a further preferred embodiment, the mutated CESA-encoding nucleic acid selected in step d) provides at least 2-fold, at least 5-fold, at least 10-fold, at least 20-fold, at least 50-fold, at least 100-fold, at least 500-fold, as much resistance or tolerance of a cell or plant to a CESA-inhibiting herbicide as compared to that provided by the control CESA-encoding nucleic acid.

The resistance or tolerance can be determined by generating a transgenic plant or host cell, preferably a plant cell, comprising a nucleic acid sequence of the library of step a) and comparing said transgenic plant with a control plant or host cell, preferably a plant cell.

Another object refers to a method of identifying a plant or algae containing a nucleic acid comprising a nucleotide sequence encoding a wildtype or mutated CESA which is resistant or tolerant to a CESA-inhibiting herbicide, the method comprising:

-   a) identifying an effective amount of a CESA-inhibiting herbicide in     a culture of plant cells or green algae that leads to death of said     cells. -   b) treating said plant cells or green algae with a mutagenizing     agent, -   c) contacting said mutagenized cells population with an effective     amount of CESA-inhibiting herbicide, identified in a), -   d) selecting at least one cell surviving these test conditions, -   e) PCR-amplification and sequencing of CESA genes from cells     selected in d) and comparing such sequences to wild-type CESA gene     sequences, respectively.

In a preferred embodiment, said mutagenizing agent is ethylmethanesulfonate (EMS).

Many methods well known to the skilled artisan are available for obtaining suitable candidate nucleic acids for identifying a nucleotide sequence encoding a wildtype or mutated CESA from a variety of different potential source organisms including microbes, plants, fungi, algae, mixed cultures etc. as well as environmental sources of DNA such as soil. These methods include inter alia the preparation of cDNA or genomic DNA libraries, the use of suitably degenerate oligonucleotide primers, the use of probes based upon known sequences or complementation assays (for example, for growth upon tyrosine) as well as the use of mutagenesis and shuffling in order to provide recombined or shuffled wildtype or mutated CESA-encoding sequences.

Nucleic acids comprising candidate and control CESA encoding sequences can be expressed in yeast, in a bacterial host strain, in an alga or in a higher plant such as tobacco or Arabidopsis and the relative levels of inherent tolerance of the CESA encoding sequences screened according to a visible indicator phenotype of the transformed strain or plant in the presence of different concentrations of the selected CESA-inhibiting herbicide. Dose responses and relative shifts in dose responses associated with these indicator phenotypes (formation of necrosis, growth inhibition, herbicidal effect etc) are conveniently expressed in terms, for example, of GR50 (concentration for 50% reduction of growth) or MIC (minimum inhibitory concentration) values where increases in values correspond to increases in inherent tolerance of the expressed CESA. For example, in a relatively rapid assay system based upon transformation of Arabidopsis as described in the Example section (Example 7), each wildtype or mutated CESA encoding sequence may be expressed, for example, as a DNA sequence under expression control of a suitable promoter and T1 plants can be selected for differential tolerance to selected CESA-inhibiting herbicides, measured by growth.

In another embodiment, candidate nucleic acids are transformed into plant material to generate a transgenic plant, regenerated into morphologically normal fertile plants which are then measured for differential tolerance to selected CESA-inhibiting herbicides as described in the Example section hereinafter. Many suitable methods for transformation using suitable selection markers such as kanamycin, binary vectors such as from Agrobacterium and plant regeneration as, for example, from tobacco leaf discs are well known in the art. Optionally, a control population of plants is likewise transformed with a nucleic acid expressing the control CESA. Alternatively, an untransformed dicot plant such as Arabidopsis or Tobacco can be used as a control since this, in any case, expresses its own endogenous CESA. The average, and distribution, of herbicide tolerance levels of a range of primary plant transformation events or their progeny to CESA-inhibiting herbicides described supra are evaluated in the normal manner based upon plant damage, meristematic bleaching symptoms etc. at a range of different concentrations of herbicides. These data can be expressed in terms of, for example, GR50 values derived from dose/response curves having “dose” plotted on the x-axis and “percentage kill”, “herbicidal effect”, “numbers of emerging green plants” etc. plotted on the y-axis where increased GR50 values correspond to increased levels of inherent tolerance of the expressed CESA. Herbicides can suitably be applied pre-emergence or post-emergence.

Another object of the present invention refers to an isolated and or recombinantly produced and/or synthetic nucleic acid encoding a mutated CESA as disclosed SUPRA, wherein the nucleic acid comprises the nucleotide sequence of SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a variant or derivative thereof.

In one embodiment, the nucleic acid is identifiable by a method as defined above.

For the purposes of the invention “recombinant” means with regard for example to a nucleic acid sequence, an expression cassette (=gene construct, nucleic acid construct) or a vector containing the nucleic acid sequence according to the invention or an organism transformed by said nucleic acid sequences, expression cassette or vector according to the invention all those constructions produced by genetic engineering methods in which either

(a) the nucleic acid sequence comprising the sequence of SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a homolog thereof, or its derivatives or parts thereof; or

(b) a genetic control sequence functionally linked to the nucleic acid sequence described under (a), for example a 3′- and/or 5′-genetic control sequence such as a promoter or terminator, or

(c) (a) and (b);

are not found in their natural, genetic environment or have been modified by genetic engineering methods, wherein the modification may by way of example be a substitution, addition, deletion, inversion or insertion of one or more nucleotide residues.

“Natural genetic environment” means the natural genomic or chromosomal locus in the organism of origin or inside the host organism or presence in a genomic library. In the case of a genomic library the natural genetic environment of the nucleic acid sequence is preferably retained at least in part. The environment borders the nucleic acid sequence at least on one side and has a sequence length of at least 50 bp, preferably at least 500 bp, particularly preferably at least 1,000 bp, most particularly preferably at least 5,000 bp. A naturally occurring expression cassette—for example the naturally occurring combination of the natural promoter of the nucleic acid sequence according to the invention with the corresponding gene—turns into a transgenic expression cassette when the latter is modified by unnatural, synthetic (“artificial”) methods such as by way of example a mutagenation. Appropriate methods are described by way of example in U.S. Pat. No. 5,565,350 or WO 00/15815

In a preferred embodiment, the encoded mutated CESA is a variant of SEQ ID NO: 1, which includes one or more of the following:

the amino acid corresponding to position 998 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp;

the amino acid corresponding to position 1009 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

the amino acid corresponding to position 1010 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Gly, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

the amino acid corresponding to position 1013 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Ser, Thr, Asn, Gln, Cys, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

the amino acid corresponding to position 1052 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp,

the amino acid corresponding to position 1055 of SEQ ID NO:1 is Arg, His, Lys, Asp, Glu, Thr, Asn, Gln, Cys, Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, or Trp.

In a preferred embodiment, the amino acid corresponding to position 1009 of SEQ ID NO: 1 is Asp.

In another preferred embodiment, the amino acid corresponding to position 1010 of SEQ ID NO: 1 is Leu.

In another preferred embodiment, the amino acid corresponding to position 1013 of SEQ ID NO: 1 is Arg.

In another preferred embodiment, the amino acid corresponding to position 983 of SEQ ID NO: 3 is Phe.

In another preferred embodiment, the amino acid corresponding to position 1037 of SEQ ID NO: 3 is Phe.

In another preferred embodiment, the amino acid corresponding to position 1040 of SEQ ID NO: 3 is Leu.

In other aspects, the present invention encompasses a progeny or a descendant of a CESA-inhibiting herbicides-tolerant plant of the present invention as well as seeds derived from the CESA-inhibiting herbicides-tolerant plants of the invention and cells derived from the CESA-inhibiting herbicides-tolerant plants of the invention.

In some embodiments, the present invention provides a progeny or descendant plant derived from a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, wherein the progeny or descendant plant comprises in at least some of its cells the recombinant polynucleotide operably linked to the promoter, the expression of the wildtype or mutated CESA polypeptide conferring to the progeny or descendant plant tolerance to the CESA-inhibiting herbicides.

In one embodiment, seeds of the present invention preferably comprise the CESA-inhibiting herbicides-tolerance characteristics of the CESA-inhibiting herbicides-tolerant plant. In other embodiments, a seed is capable of germination into a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the progeny or descendant plant tolerance to the CESA-inhibiting herbicides.

In some embodiments, plant cells of the present invention are capable of regenerating a plant or plant part. In other embodiments, plant cells are not capable of regenerating a plant or plant part. Examples of cells not capable of regenerating a plant include, but are not limited to, endosperm, seed coat (testa & pericarp), and root cap.

In another embodiment, the present invention provides a plant cell of or capable of regenerating a plant comprising in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to the CESA-inhibiting herbicides, wherein the plant cell comprises the recombinant polynucleotide operably linked to a promoter.

In other embodiments, the present invention provides a plant cell comprising a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the cell tolerance to the CESA-inhibiting herbicides.

In another embodiment, the invention refers to a plant cell transformed by a nucleic acid encoding a wildtype or mutated CESA polypeptide according to the present invention or to a plant cell which has been mutated to obtain a plant expressing a nucleic acid encoding a wildtype or mutated CESA polypeptide according to the present invention, wherein expression of the nucleic acid in the plant cell results in increased resistance or tolerance to a CESA-inhibiting herbicide as compared to a wild type variety of the plant cell. Preferably, the wildtype or mutated CESA polypeptide encoding nucleic acid comprises a polynucleotide sequence selected from the group consisting of: a) a polynucleotide as shown in SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a variant or derivative thereof; b) a polynucleotide encoding a polypeptide as shown in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, or a variant or derivative thereof; c) a polynucleotide comprising at least 60 consecutive nucleotides of any of a) or b); and d) a polynucleotide complementary to the polynucleotide of any of a) through c).

In some aspects, the present invention provides a plant product prepared from the CESA-inhibiting herbicides-tolerant plants hereof. In some embodiments, examples of plant products include, without limitation, grain, oil, and meal. In one embodiment, a plant product is plant grain (e.g., grain suitable for use as feed or for processing), plant oil (e.g., oil suitable for use as food or biodiesel), or plant meal (e.g., meal suitable for use as feed).

In one embodiment, a plant product prepared from a plant or plant part is provided, wherein the plant or plant part comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the a plant or plant part tolerance to the CESA-inhibiting herbicides.

In another embodiment, the invention refers to a method of producing a transgenic plant cell with an increased resistance to a CESA-inhibiting herbicide as compared to a wild type variety of the plant cell comprising, transforming the plant cell with an expression cassette comprising a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide.

In another embodiment, the invention refers to a method of producing a transgenic plant comprising, (a) transforming a plant cell with an expression cassette comprising a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, and (b) generating a plant with an increased resistance to CESA-inhibiting herbicide from the plant cell.

In some aspects, the present invention provides a method for producing a CESA-inhibiting herbicides-tolerant plant. In one embodiment, the method comprises: regenerating a plant from a plant cell transformed with a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to the CESA-inhibiting herbicides.

The term “expression/expressing” or “gene expression” means the transcription of a specific gene or specific genes or specific genetic construct. The term “expression” or “gene expression” in particular means the transcription of a gene or genes or genetic construct into structural RNA (rRNA, tRNA) or mRNA with or without subsequent translation of the latter into a protein. The process includes transcription of DNA and processing of the resulting mRNA product.

To obtain the desired effect, i.e. plants that are tolerant or resistant to the CESA-inhibiting herbicide derivative herbicide of the present invention, it will be understood that the at least one nucleic acid is “over-expressed” by methods and means known to the person skilled in the art.

The term “increased expression” or “overexpression” as used herein means any form of expression that is additional to the original wild-type expression level. Methods for increasing expression of genes or gene products are well documented in the art and include, for example, overexpression driven by appropriate promoters, the use of transcription enhancers or translation enhancers. Isolated nucleic acids which serve as promoter or enhancer elements may be introduced in an appropriate position (typically upstream) of a non-heterologous form of a polynucleotide so as to upregulate expression of a nucleic acid encoding the polypeptide of interest. For example, endogenous promoters may be altered in vivo by mutation, deletion, and/or substitution (see, Kmiec, U.S. Pat. No. 5,565,350; Zarling et al., WO9322443), or isolated promoters may be introduced into a plant cell in the proper orientation and distance from a gene of the present invention so as to control the expression of the gene. If polypeptide expression is desired, it is generally desirable to include a polyadenylation region at the 3′-end of a polynucleotide coding region. The polyadenylation region can be derived from the natural gene, from a variety of other plant genes, or from T-DNA. The 3′ end sequence to be added may be derived from, for example, the nopaline synthase or octopine synthase genes, or alternatively from another plant gene, or less preferably from any other eukaryotic gene. An intron sequence may also be added to the 5′ untranslated region (UTR) or the coding sequence of the partial coding sequence to increase the amount of the mature message that accumulates in the cytosol. Inclusion of a spliceable intron in the transcription unit in both plant and animal expression constructs has been shown to increase gene expression at both the mRNA and protein levels up to 1000-fold (Buchman and Berg (1988) Mol. Cell biol. 8: 4395-4405; Callis et al. (1987) Genes Dev 1:1183-1200). Such intron enhancement of gene expression is typically greatest when placed near the 5′ end of the transcription unit. Use of the maize introns Adh1-S intron 1, 2, and 6, the Bronze-1 intron are known in the art. For general information see: The Maize Handbook, Chapter 116, Freeling and Walbot, Eds., Springer, N.Y. (1994).

Where appropriate, nucleic acid sequences may be optimized for increased expression in a transformed plant. For example, coding sequences that comprise plant-preferred codons for improved expression in a plant can be provided. See, for example, Campbell and Gowri (1990) Plant Physiol., 92: 1-11 for a discussion of host-preferred codon usage. Methods also are known in the art for preparing plant-preferred genes. See, for example, U.S. Pat. Nos. 5,380,831, and 5,436,391, and Murray et al. (1989) Nucleic Acids Res. 17:477-498, herein incorporated by reference.

Consequently, wildtype or mutated CESA nucleic acids of the invention are provided in expression cassettes for expression in the plant of interest. The cassette will include regulatory sequences operably linked to a wildtype or mutated CESA nucleic acid sequence of the invention. The term “regulatory element” as used herein refers to a polynucleotide that is capable of regulating the transcription of an operably linked polynucleotide. It includes, but not limited to, promoters, enhancers, introns, 5′ UTRs, and 3′ UTRs. By “operably linked” is intended a functional linkage between a promoter and a second sequence, wherein the promoter sequence initiates and mediates transcription of the DNA sequence corresponding to the second sequence. Generally, operably linked means that the nucleic acid sequences being linked are contiguous and, where necessary to join two protein coding regions, contiguous and in the same reading frame. The cassette may additionally contain at least one additional gene to be cotransformed into the organism. Alternatively, the additional gene(s) can be provided on multiple expression cassettes.

Such an expression cassette is provided with a plurality of restriction sites for insertion of the wildtype or mutated CESA nucleic acid sequence to be under the transcriptional regulation of the regulatory regions. The expression cassette may additionally contain selectable marker genes. The expression cassette of the present invention will include in the 5′-3′ direction of transcription, a transcriptional and translational initiation region (i.e., a promoter), a wildtype or mutated CESA encoding nucleic acid sequence of the invention, and a transcriptional and translational termination region (i.e., termination region) functional in plants. The promoter may be native or analogous, or foreign or heterologous, to the plant host and/or to the wildtype or mutated CESA nucleic acid sequence of the invention. Additionally, the promoter may be the natural sequence or alternatively a synthetic sequence. Where the promoter is “foreign” or “heterologous” to the plant host, it is intended that the promoter is not found in the native plant into which the promoter is introduced. Where the promoter is “foreign” or “heterologous” to the wildtype or mutated CESA nucleic acid sequence of the invention, it is intended that the promoter is not the native or naturally occurring promoter for the operably linked wildtype or mutated CESA nucleic acid sequence of the invention. As used herein, a chimeric gene comprises a coding sequence operably linked to a transcription initiation region that is heterologous to the coding sequence. While it may be preferable to express the wildtype or mutated CESA nucleic acids of the invention using heterologous promoters, the native promoter sequences may be used. Such constructs would change expression levels of the wildtype or mutated CESA protein in the plant or plant cell. Thus, the phenotype of the plant or plant cell is altered.

The termination region may be native with the transcriptional initiation region, may be native with the operably linked wildtype or mutated CESA sequence of interest, may be native with the plant host, or may be derived from another source (i.e., foreign or heterologous to the promoter, the wildtype or mutated CESA nucleic acid sequence of interest, the plant host, or any combination thereof). Convenient termination regions are available from the Ti-plasmid of A. tumefaciens, such as the octopine synthase and nopaline synthase termination regions. See also Guerineau et al. (1991) Mol. Gen. Genet. 262: 141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991) Genes Dev. 5: 141-149; Mogen et al. (1990) Plant Cell 2: 1261-1272; Munroe et al. (1990) Gene 91: 151-158; Ballas t al. (1989) Nucleic Acids Res. 17:7891-7903; and Joshi et al. (1987) Nucleic Acid Res. 15:9627-9639. Where appropriate, the gene(s) may be optimized for increased expression in the transformed plant. That is, the genes can be synthesized using plant-preferred codons for improved expression. See, for example, Campbell and Gowri (1990) Plant Physiol. 92: 1-11 for a discussion of host-preferred codon usage. Methods are available in the art for synthesizing plant-preferred genes. See, for example, U.S. Pat. Nos. 5,380,831, and 5,436,391, and Murray et al. (1989) Nucleic Acids Res. 17:477-498, herein incorporated by reference.

While the polynucleotides of the invention may find use as selectable marker genes for plant transformation, the expression cassettes of the invention can include another selectable marker gene for the selection of transformed cells. Selectable marker genes, including those of the present invention, are utilized for the selection of transformed cells or tissues. Marker genes include, but are not limited to, genes encoding antibiotic resistance, such as those encoding neomycin phosphotransferase II (NEO) and hygromycin phosphotransferase (HPT), as well as genes conferring resistance to herbicidal compounds, such as glufosinate ammonium, bromoxynil, imidazolinones, and 2,4-dichlorophenoxyacetate (2,4-D). See generally, Yarranton (1992) Curr. Opin. Biotech. 3:506-511; Christophers on et al (1992) Proc. Natl. Acad. ScL USA 89:6314-6318; Yao et al. (1992) Cell 71:63-72; Reznikoff (1992) Mol Microbiol 6:2419-2422; Barkley et al (1980) in The Operon, pp. 177-220; Hu et al (1987) Cell 48:555-566; Brown et al (1987) Cell 49:603-612; Figge et al (1988) Cell 52:713-722; Deuschle et al (1989) Proc. Natl Acad. AcL USA 86:5400-5404; Fuerst et al (1989) Proc. Natl Acad. ScL USA 86:2549-2553; Deuschle et al (1990) Science 248:480-483; Gossen (1993) Ph.D. Thesis, University of Heidelberg; Reines et al (1993) Proc. Natl Acad. ScL USA 90: 1917-1921; Labow et al (1990) Mol Cell Biol 10:3343-3356; Zambretti et al (1992) Proc. Natl Acad. ScL USA 89:3952-3956; Bairn et al (1991) Proc. Natl Acad. ScL USA 88:5072-5076; Wyborski et al (1991) Nucleic Acids Res. 19:4647-4653; Hillenand-Wissman (1989) Topics Mol Struc. Biol 10: 143-162; Degenkolb et al (1991) Antimicrob. Agents Chemother. 35: 1591-1595; Kleinschnidt et al (1988) Biochemistry 27: 1094-1104; Bonin (1993) Ph.D. Thesis, University of Heidelberg; Gossen et al (1992) Proc. Natl Acad. ScL USA 89:5547-5551; Oliva et al (1992) Antimicrob. Agents Chemother. 36:913-919; Hlavka et al (1985) Handbook of Experimental Pharmacology, Vol. 78 (Springer-Verlag, Berlin); Gill et al (1988) Nature 334:721-724. Such disclosures are herein incorporated by reference. The above list of selectable marker genes is not meant to be limiting. Any selectable marker gene can be used in the present invention.

Further, additional sequence modifications are known to enhance gene expression in a cellular host. These include elimination of sequences encoding spurious polyadenylation signals, exon-intron splice site signals, transposon-like repeats, and other such well-characterized sequences that may be deleterious to gene expression. The G-C content of the sequence may be adjusted to levels average for a given cellular host, as calculated by reference to known genes expressed in the host cell. Also, if desired, sequences can be readily modified to avoid predicted hairpin secondary mRNA structures. Nucleotide sequences for enhancing gene expression can also be used in the plant expression vectors. These include, for example, introns of the maize Adh gene Adh1-S intron 1, 2, and 6 (Callis et al. Genes and Development 1: 1183-1200, 1987), and leader sequences, (W-sequence) from the Tobacco Mosaic virus (TMV), Maize Chlorotic Mottle Virus and Alfalfa Mosaic Virus (Gallie et al. Nucleic Acid Res. 15:8693-8711, 1987 and Skuzeski et al. Plant Mol. Biol. 15:65-79, 1990). The first intron from the shrunken-1 locus of maize has been shown to increase expression of genes in chimeric gene constructs. U.S. Pat. Nos. 5,424,412 and 5,593,874 disclose the use of specific introns in gene expression constructs, and Gallie et al. (Plant Physiol. 106:929-939, 1994) also have shown that introns are useful for regulating gene expression on a tissue specific basis. To further enhance or to optimize gene expression, the plant expression vectors of the invention also may contain DNA sequences containing matrix attachment regions (MARs). Plant cells transformed with such modified expression systems, then, may exhibit overexpression or constitutive expression of a nucleotide sequence of the invention.

The invention further provides an isolated recombinant expression vector comprising the expression cassette containing a wildtype or mutated CESA nucleic acid nucleic acid as described above, wherein expression of the vector in a host cell results in increased tolerance to a CESA-inhibiting herbicide as compared to a wild type variety of the host cell. As used herein, the term “vector” refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a “plasmid,” which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as “expression vectors.” In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, “plasmid” and “vector” can be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses, and adeno-associated viruses), which serve equivalent functions.

The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operably linked to the nucleic acid sequence to be expressed. Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cells and those that direct expression of the nucleotide sequence only in certain host cells or under certain conditions. It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of polypeptide desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce polypeptides or peptides, including fusion polypeptides or peptides, encoded by nucleic acids as described herein (e.g., wildtype or mutated CESA polypeptides, fusion polypeptides, etc.)

Expression vectors may additionally contain 5′ leader sequences in the expression construct. Such leader sequences can act to enhance translation. Translation leaders are known in the art and include: picornavirus leaders, for example, EMCV leader (Encephalomyo carditis 5′ noncoding region) (Elroy-Stein et al. (1989) PNAS, 86:6126-6130); potyvirus leaders, for example, TEV leader (Tobacco Etch Virus) (Gallie et al. (1995) Gene 165(2):233-238), MDMV leader (Maize Dwarf Mosaic Virus) (Virology 154:9-20), and human immunoglobulin heavy-chain binding protein (BiP) (Macejak et al. (1991) Nature 353:90-94); untranslated leader from the coat protein mRNA of alfalfa mosaic virus (AMV RNA 4) (Jobling et al. (1987) Nature 325:622-625); tobacco mosaic virus leader (TMV) (Gallie et al. (1989) in Molecular Biology of RNA, ed. Cech (Liss, New York), pp. 237-256); and maize chlorotic mottle virus leader (MCMV) (Lommel et al. (1991) Virology 81:382-385). See also, Della-Cioppa et al. (1987) Plant Physiol. 84:965-968.

Other methods known to enhance translation also can be utilized, for example, introns, and the like. In preparing an expression vector, the various nucleic acid fragments may be manipulated, so as to provide for the nucleic acid sequences in the proper orientation and, as appropriate, in the proper reading frame. Toward this end, adapters or linkers may be employed to join the nucleic acid fragments or other manipulations may be involved to provide for convenient restriction sites, removal of superfluous nucleic acid, removal of restriction sites, or the like. For this purpose, in vitro mutagenesis, primer repair, restriction, annealing, resubstitutions, e.g., transitions and transversions, may be involved.

A number of promoters can be used in the practice of the invention. The promoters can be selected based on the desired outcome. The nucleic acids can be combined with constitutive, tissue-preferred, or other promoters for expression in plants.

Constitutive promoters include, for example, the core promoter of the Rsyn7 promoter and other constitutive promoters disclosed in WO 99/43838 and U.S. Pat. No. 6,072,050; the core CaMV 35S promoter (Odell et al. (1985) Nature 313:810-812); rice actin (McElroy et al. (1990) Plant Cell 2: 163-171); ubiquitin (Christensen et al. (1989) Plant Mol. Biol. 12:619-632 and Christensen et al. (1992) Plant Mol. Biol. 18:675-689); pEMU (Last et al. (1991) Theor. Appl. Genet. 81:581-588); MAS (Velten et al. (1984) EMBO J. 3:2723-2730); ALS promoter (U.S. Pat. No. 5,659,026), and the like. Other constitutive promoters include, for example, U.S. Pat. Nos. 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785; 5,399,680; 5,268,463; 5,608,142; and 6,177,611.

Tissue-preferred promoters can be utilized to target enhanced expression within a particular plant tissue. Such tissue-preferred promoters include, but are not limited to, leaf-preferred promoters, root-preferred promoters, seed-preferred promoters, and stem-preferred promoters. Some examples of tissue-preferred promoters are described by, e.g., Yamamoto et al. (1997) Plant J. 12(2):255-265; Kawamata et al. (1997) Plant Cell Physiol. 38(7):792-803; Hansen et al. (1997) Mol. Gen Genet. 254(3):337-343; Russell et al. (1997) Transgenic Res. 6(2): 157-168; Rinehart et al. (1996) Plant Physiol. 1 12(3): 1331-1341; Van Camp et al. (1996) Plant Physiol. 112(2):525-535; Canevascini et al. (1996) Plant Physiol. 1 12(2):513-524; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778; Lam (1994) Results Probl. Cell Differ. 20:181-196; Orozco et al. (1993) Plant Mol Biol. 23(6): 1 129-1138; Matsuoka et al. (1993) Voc Natl. Acad. ScL USA 90(20):9586-9590; and Guevara-Garcia et al. (1993) Plant J 4(3):495-505. Promoters can be modified, if necessary, for weak expression.

In some embodiments, the nucleic acids of interest can be targeted to the chloroplast for expression. In this manner, where the nucleic acid of interest is not directly inserted into the chloroplast, the expression vector will additionally contain a chloroplast-targeting sequence comprising a nucleotide sequence that encodes a chloroplast transit peptide to direct the gene product of interest to the chloroplasts. Such transit peptides are known in the art. With respect to chloroplast-targeting sequences, “operably linked” means that the nucleic acid sequence encoding a transit peptide (i.e., the chloroplast-targeting sequence) is linked to the desired coding sequence of the invention such that the two sequences are contiguous and in the same reading frame. See, for example, Von Heijne et al. (1991) Plant Mol. Biol. Rep. 9: 104-126; Clark et al. (1989) J Biol. Chem. 264:17544-17550; Della-Cioppa et al. (1987) Plant Physiol. 84:965-968; Romer et al. (1993) Biochem. Biophys. Res. Commun. 196: 1414-1421; and Shah et al. (1986) Science 233:478-481. For example, a chloroplast transit peptide known in the art can be fused to the amino acid sequence of a CESA polypeptide of the invention by operably linking a chloroplast-targeting sequence to the 5′-end of a nucleotide sequence encoding the CESA polypeptide.

Chloroplast targeting sequences are known in the art and include the chloroplast small subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) (de Castro Silva Filho et al. (1996) Plant Mol. Biol. 30:769-780; Schnell et al. (1991) J Biol. Chem. 266(5):3335-3342); EPSPS (Archer et al. (1990) J Bioenerg. Biomemb. 22(6):789-810); tryptophan synthase (Zhao et al. (1995) J Biol. Chem. 270(11):6081-6087); plastocyanin (Lawrence et al. (1997) J Biol. Chem. 272(33):20357-20363); chorismate synthase (Schmidt et al. (1993) J Biol. Chem. 268(36):27447-27457); and the light harvesting chlorophyll a/b binding protein (LHBP) (Lamppa et al. (1988) J Biol. Chem. 263: 14996-14999). See also Von Heijne et al. (1991) Plant Mol. Biol. Rep. 9: 104-126; Clark et al. (1989) J Biol. Chem. 264: 17544-17550; Della-Cioppa et al. (1987) Plant Physiol. 84:965-968; Romer et al. (1993) Biochem Biophys. Res. Commun. 196: 1414-1421; and Shah et al. (1986) Science 233:478-481.

Methods for transformation of chloroplasts are known in the art. See, for example, Svab et al. (1990) Proc. Natl. Acad. ScL USA 87:8526-8530; Svab and Maliga (1993) Proc. Natl. Acad. Sci. USA 90:913-917; Svab and Maliga (1993) EMBO J. 12:601-606. The method relies on particle gun delivery of DNA containing a selectable marker and targeting of the DNA to the plastid genome through homologous recombination. Additionally, plastid transformation can be accomplished by transactivation of a silent plastid-borne transgene by tissue-preferred expression of a nuclear-encoded and plastid-directed RNA polymerase. Such a system has been reported in McBride et al. (1994) Proc. Natl. Acad. Sci. USA 91:7301-7305.

The nucleic acids of interest to be targeted to the chloroplast may be optimized for expression in the chloroplast to account for differences in codon usage between the plant nucleus and this organelle. In this manner, the nucleic acids of interest may be synthesized using chloroplast-preferred codons. See, for example, U.S. Pat. No. 5,380,831, herein incorporated by reference.

Numerous plant transformation vectors and methods for transforming plants are available. See, for example, An, G. et al. (1986) Plant PysioL, 81:301-305; Fry, J., et al. (1987) Plant Cell Rep. 6:321-325; Block, M. (1988) Theor. Appl. Genet. 16: 161-11 A; Hinchee, et al. (1990) Stadler. Genet. Symp. 20322.203-22; Cousins, et al. (1991) Aust. J. Plant Physiol. 18:481-494; Chee, P. P. and Slightom, J. L. (1992) Gene.I I 8:255-260; Christou, et al. (1992) Trends. Biotechnol. 10:239-246; Halluin, et al. (1992) Bio/Technol. 10:309-314; Dhir, et al. (1992) Plant Physiol. 99:81-88; Casas et al. (1993) Proc. Nat. Acad Sd. USA 90: 1 1212-1 1216; Christou, P. (1993) In Vitro Cell. Dev. Biol.-Plant; 29P. 119-124; Davies, et al. (1993) Plant Cell Rep. 12: 180-183; Dong, J. A. and Mchughen, A. (1993) Plant ScL 91: 139-148; Franklin, C. I. and Trieu, T. N. (1993) Plant. Physiol. 102: 167; Golovkin, et al. (1993) Plant ScL 90:41-52; Guo Chin ScL Bull. 38:2072-2078; Asano, et al. (1994) Plant Cell Rep. 13; Ayeres N. M. and Park, W. D. (1994) Crit. Rev. Plant. Sci. 13:219-239; Barcelo, et al. (1994) Plant. J. 5:583-592; Becker, et al. (1994) Plant. J. 5:299-307; Borkowska et al. (1994) Acta. Physiol Plant. 16:225-230; Christou, P. (1994) Agro. Food. Ind. Hi Tech. 5: 17-27; Eapen et al. (1994) Plant Cell Rep. 13:582-586; Hartman, et al. (1994) Bio-Technology 12: 919923; Ritala, et al. (1994) Plant. Mol. Biol. 24:317-325; and Wan, Y. C. and Lemaux, P. G. (1994) Plant Physiol. 104:3748.

In some embodiments, the methods of the invention involve introducing a polynucleotide construct into a plant. By “introducing” is intended presenting to the plant the polynucleotide construct in such a manner that the construct gains access to the interior of a cell of the plant. The methods of the invention do not depend on a particular method for introducing a polynucleotide construct to a plant, only that the polynucleotide construct gains access to the interior of at least one cell of the plant. Methods for introducing polynucleotide constructs into plants are known in the art including, but not limited to, stable transformation methods, transient transformation methods, and virus-mediated methods. The term “introduction” or “transformation” as referred to herein further means the transfer of an exogenous polynucleotide into a host cell, irrespective of the method used for transfer. Plant tissue capable of subsequent clonal propagation, whether by organogenesis or embryogenesis, may be transformed with a genetic construct of the present invention and a whole plant regenerated there from. The particular tissue chosen will vary depending on the clonal propagation systems available for, and best suited to, the particular species being transformed. Exemplary tissue targets include leaf disks, pollen, embryos, cotyledons, hypocotyls, megagametophytes, callus tissue, existing meristematic tissue (e.g., apical meristem, axillary buds, and root meristems), and induced meristem tissue (e.g., cotyledon meristem and hypocotyl meristem). The polynucleotide may be transiently or stably introduced into a host cell and may be maintained non-integrated, for example, as a plasmid. Alternatively, it may be integrated into the host genome. The resulting transformed plant cell may then be used to regenerate a transformed plant in a manner known to persons skilled in the art.

By “stable transformation” is intended that the polynucleotide construct introduced into a plant integrates into the genome of the plant and is capable of being inherited by descendent thereof. By “transient transformation” is intended that a polynucleotide construct introduced into a plant does not integrate into the genome of the plant.

For the transformation of plants and plant cells, the nucleotide sequences of the invention are inserted using standard techniques into any vector known in the art that is suitable for expression of the nucleotide sequences in a plant or plant cell. The selection of the vector depends on the preferred transformation technique and the target plant species to be transformed. In an embodiment of the invention, the encoding nucleotide sequence is operably linked to a plant promoter, e.g. a promoter known in the art for high-level expression in a plant cell, and this construct is then introduced into a plant cell that is susceptible to CESA-inhibiting herbicides; and a transformed plant is regenerated. In some embodiments, the transformed plant is tolerant to exposure to a level of CESA-inhibiting herbicides that would kill or significantly injure a plant regenerated from an untransformed cell. This method can be applied to any plant species or crops.

Methodologies for constructing plant expression vectors and introducing foreign nucleic acids into plants are generally known in the art. For example, foreign DNA can be introduced into plants, using tumor-inducing (Ti) plasmid vectors. Other methods utilized for foreign DNA delivery involve the use of PEG mediated protoplast transformation, electroporation, microinjection whiskers, and biolistics or microprojectile bombardment for direct DNA uptake. Such methods are known in the art. (U.S. Pat. No. 5,405,765 to Vasil et al.; Bilang et al. (1991) Gene 100: 247-250; Scheid et al., (1991) MoL Gen. Genet., 228: 104-1 12; Guerche et al., (1987) Plant Science 52: 111-116; Neuhause et al., (1987) Theor. Appl Genet. 75: 30-36; Klein et al., (1987) Nature 327: 70-73; Howell et al., (1980) Science 208: 1265; Horsch et al., (1985) Science 227: 1229-1231; DeBlock et al., (1989) Plant Physiology 91: 694-701; Methods for Plant Molecular Biology (Weissbach and Weissbach, eds.) Academic Press, Inc. (1988) and Methods in Plant Molecular Biology (Schuler and Zielinski, eds.) Academic Press, Inc. (1989).

Other suitable methods of introducing nucleotide sequences into plant cells include microinjection as described by e.g., Crossway et al. (1986) Biotechniques 4:320-334, electroporation as described by e.g., Riggs et al. (1986) Proc. Natl. Acad. ScL USA 83:5602-5606, Agrobacterium-mediated transformation as described by e.g., Townsend et al., U.S. Pat. No. 5,563,055, Zhao et al., U.S. Pat. No. 5,981,840, direct gene transfer as described by, e.g., Paszkowski et al. (1984) EMBO J. 3:2717-2722, and ballistic particle acceleration as described by, e.g., U.S. Pat. Nos. 4,945,050; 5,879,918; 5,886,244; and 5,932,782; Tomes et al. (1995) “Direct DNA Transfer into Intact Plant Cells via Microprojectile Bombardment,” in Plant Cell, Tissue, and Organ Culture: Fundamental Methods, ed. Gamborg and Phillips (Springer-Verlag, Berlin); McCabe et al. (1988) Biotechnology 6:923-926); and Led transformation (WO 00/28058). Also see, Weissinger et al., (1988) Ann. Rev. Genet. 22:421-477; Sanford et al, (1987) Particulate Science and Technology 5:27-37 (onion); Christou et al, (1988) Plant Physiol. 87:671-674 (soybean); McCabe et al., (1988) Bio/Technology 6:923-926 (soybean); Finer and McMullen (1991) In Vitro Cell Dev. Biol. 27P: 175-182 (soybean); Singh et al, (1998) Theor. Appl. Genet. 96:319-324 (soybean); Datta et al., (1990) Biotechnology 8:736-740 (rice); Klein et al., (1988) PNAS, 85:4305-4309 (maize); Klein et al., (1988) Biotechnology 6:559-563 (maize); U.S. Pat. Nos. 5,240,855; 5,322,783; and 5,324,646; Tomes et al., (1995) “Direct DNA Transfer into Intact Plant Cells via Microprojectile Bombardment,” in Plant Cell, Tissue, and Organ Culture: Fundamental Methods, ed. Gamborg (Springer-Verlag, Berlin) (maize); Klein et al., (1988) Plant Physiol. 91:440-444 (maize); Fromm et al., (1990) Biotechnology 8:833-839 (maize); Hooykaas-Van Slogteren et al., (1984) Nature (London) 31 1:763-764; Bowen et al, U.S. Pat. No. 5,736,369 (cereals); Bytebier et al, (1987) PNAS 84:5345-5349 (Liliaceae); De Wet et al., (1985) in The Experimental Manipulation of Ovule Tissues, ed. Chapman et al, (Longman, N.Y.), pp. 197-209 (pollen); Kaeppler et al., (1990) Plant Cell Reports 9:415-418 and Kaeppler et al., (1992) Theor. Apph Genet. 84:560-566 (whisker-mediated transformation); D'Halluin et al., (1992) Plant Cell 4: 1495-1505 (electroporation); Li et al., (1993) Plant Cell Reports 12:250-255 and Christou and Ford (1995) Annals of Botany 75:407-413 (rice); Osjoda et al, (1996) Nature Biotechnology 14:745-750 (maize via Agrobacterium tumefaciens); each of which is herein incorporated by reference.

Transgenic plants, including transgenic crop plants, are preferably produced via Agrobacterium-mediated transformation. An advantageous transformation method is the transformation in planta. To this end, it is possible, for example, to allow the agrobacteria to act on plant seeds or to inoculate the plant meristem with agrobacteria. It has proved particularly expedient in accordance with the invention to allow a suspension of transformed agrobacteria to act on the intact plant or at least on the flower primordia. The plant is subsequently grown on until the seeds of the treated plant are obtained (Clough and Bent, Plant J. (1998) 16, 735-743). Methods for Agrobacterium-mediated transformation of rice include well known methods for rice transformation, such as those described in any of the following: European patent application EP 1198985 A1, Aldemita and Hodges (Planta 199: 612-617, 1996); Chan et al. (Plant Mol Biol 22 (3): 491-506, 1993), Hiei et al. (Plant J 6 (2): 271-282, 1994), which disclosures are incorporated by reference herein as if fully set forth. In the case of corn transformation, the preferred method is as described in either Ishida et al. (Nat. Biotechnol 14(6): 745-50, 1996) or Frame et al. (Plant Physiol 129(1): 13-22, 2002), which disclosures are incorporated by reference herein as if fully set forth. Said methods are further described by way of example in B. Jenes et al., Techniques for Gene Transfer, in: Transgenic Plants, Vol. 1, Engineering and Utilization, eds. S.D. Kung and R. Wu, Academic Press (1993) 128-143 and in Potrykus Annu. Rev. Plant Physiol. Plant Molec. Biol. 42 (1991) 205-225). The nucleic acids or the construct to be expressed is preferably cloned into a vector, which is suitable for transforming Agrobacterium tumefaciens, for example pBin19 (Bevan et al., Nucl. Acids Res. 12 (1984) 8711). Agrobacteria transformed by such a vector can then be used in known manner for the transformation of plants, such as plants used as a model, like Arabidopsis (Arabidopsis thaliana is within the scope of the present invention not considered as a crop plant), or crop plants such as, by way of example, tobacco plants, for example by immersing bruised leaves or chopped leaves in an agrobacterial solution and then culturing them in suitable media. The transformation of plants by means of Agrobacterium tumefaciens is described, for example, by Höfgen and Willmitzer in Nucl. Acid Res. (1988) 16, 9877 or is known inter alia from F. F. White, Vectors for Gene Transfer in Higher Plants; in Transgenic Plants, Vol. 1, Engineering and Utilization, eds. S.D. Kung and R. Wu, Academic Press, 1993, pp. 15-38.

One transformation method known to those of skill in the art is the dipping of a flowering plant into an Agrobacteria solution, wherein the Agrobacteria contains the CESA nucleic acid, followed by breeding of the transformed gametes. Agrobacterium mediated plant transformation can be performed using for example the GV3101(pMP90) (Koncz and Schell, 1986, Mol. Gen. Genet. 204:383-396) or LBA4404 (Clontech) Agrobacterium tumefaciens strain. Transformation can be performed by standard transformation and regeneration techniques (Deblaere et al., 1994, Nucl. Acids. Res. 13:4777-4788; Gelvin, Stanton B. and Schilperoort, Robert A, Plant Molecular Biology Manual, 2nd Ed.—Dordrecht: Kluwer Academic Publ., 1995.—in Sect., Ringbuc Zentrale Signatur: BT11-P ISBN 0-7923-2731-4; Glick, Bernard R. and Thompson, John E., Methods in Plant Molecular Biology and Biotechnology, Boca Raton: CRC Press, 1993 360 S., ISBN 0-8493-5164-2). For example, rapeseed can be transformed via cotyledon or hypocotyl transformation (Moloney et al., 1989, Plant Cell Report 8:238-242; De Block et al., 1989, Plant Physiol. 91:694-701). Use of antibiotics for Agrobacterium and plant selection depends on the binary vector and the Agrobacterium strain used for transformation. Rapeseed selection is normally performed using kanamycin as selectable plant marker. Agrobacterium mediated gene transfer to flax can be performed using, for example, a technique described by Mlynarova et al., 1994, Plant Cell Report 13:282-285. Additionally, transformation of soybean can be performed using for example a technique described in European Patent No. 0424 047, U.S. Pat. No. 5,322,783, European Patent No. 0397 687, U.S. Pat. No. 5,376,543, or U.S. Pat. No. 5,169,770. Transformation of maize can be achieved by particle bombardment, polyethylene glycol mediated DNA uptake, or via the silicon carbide fiber technique. (See, for example, Freeling and Walbot “The maize handbook” Springer Verlag: New York (1993) ISBN 3-540-97826-7). A specific example of maize transformation is found in U.S. Pat. No. 5,990,387, and a specific example of wheat transformation can be found in PCT Application No. WO 93/07256.

In some embodiments, polynucleotides of the present invention may be introduced into plants by contacting plants with a virus or viral nucleic acids. Generally, such methods involve incorporating a polynucleotide construct of the invention within a viral DNA or RNA molecule. It is recognized that the polypeptides of the invention may be initially synthesized as part of a viral polyprotein, which later may be processed by proteolysis in vivo or in vitro to produce the desired recombinant polypeptide. Further, it is recognized that promoters of the invention also encompass promoters utilized for transcription by viral RNA polymerases. Methods for introducing polynucleotide constructs into plants and expressing a protein encoded therein, involving viral DNA or RNA molecules, are known in the art. See, for example, U.S. Pat. Nos. 5,889,191, 5,889,190, 5,866,785, 5,589,367 and 5,316,931; herein incorporated by reference. The cells that have been transformed may be grown into plants in accordance with conventional ways. See, for example, McCormick et al. (1986) Plant Cell Reports 5:81-84. These plants may then be grown, and either pollinated with the same transformed strain or different strains, and the resulting hybrid having constitutive expression of the desired phenotypic characteristic identified. Two or more generations may be grown to ensure that expression of the desired phenotypic characteristic is stably maintained and inherited and then seeds harvested to ensure expression of the desired phenotypic characteristic has been achieved.

The present invention may be used for transformation of any plant species, including, but not limited to, monocots and dicots. Examples of plant species of interest include, but are not limited to, corn or maize (Zea mays), Brassica sp. (e.g., B. napus, B. rapa, B. juncea), particularly those Brassica species useful as sources of seed oil, alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), millet e.g., pearl millet (Pennisetum glaucum), proso millet (Panicum miliaceum), foxtail millet (Setaria italica), finger millet (Eleusine coracana)), sunflower (Helianthus annu), saffiower (Carthamus tinctorius), wheat (Triticum aestivum, T. Turgidum ssp. durum), soybean (Glycine max), tobacco (Nicotiana tabacum), potato (Solarium tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium barbadense, Gossypium hirsutum), sweet potato (Ipomoea batatus), cassava (Manihot esculenta), coffee (Coffea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea americana), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), sugarcane (Saccharum spp.), oats, barley, vegetables, ornamentals, and conifers. Preferably, plants of the present invention are crop plants (for example, sunflower, Brassica sp., cotton, sugar, beet, soybean, peanut, alfalfa, safflower, tobacco, corn, rice, wheat, rye, barley triticale, sorghum, millet, etc.).

In addition to the transformation of somatic cells, which then have to be regenerated into intact plants, it is also possible to transform the cells of plant meristems and in particular those cells which develop into gametes. In this case, the transformed gametes follow the natural plant development, giving rise to transgenic plants. Thus, for example, seeds of Arabidopsis are treated with agrobacteria and seeds are obtained from the developing plants of which a certain proportion is transformed and thus transgenic [Feldman, K A and Marks M D (1987). Mol Gen Genet 208:274-289; Feldmann K (1992). In: C Koncz, N-H Chua and J Shell, Eds, Methods in Arabidopsis Research. Word Scientific, Singapore, pp. 274-289]. Alternative methods are based on the repeated removal of the inflorescences and incubation of the excision site in the center of the rosette with transformed agrobacteria, whereby transformed seeds can likewise be obtained at a later point in time (Chang (1994). Plant J. 5: 551-558; Katavic (1994). Mol Gen Genet, 245: 363-370). However, an especially effective method is the vacuum infiltration method with its modifications such as the “floral dip” method. In the case of vacuum infiltration of Arabidopsis, intact plants under reduced pressure are treated with an agrobacterial suspension [Bechthold, N (1993). C R Acad Sci Paris Life Sci, 316: 1194-1199], while in the case of the “floral dip” method the developing floral tissue is incubated briefly with a surfactant-treated agrobacterial suspension [Clough, S J and Bent A F (1998) The Plant J. 16, 735-743]. A certain proportion of transgenic seeds are harvested in both cases, and these seeds can be distinguished from non-transgenic seeds by growing under the above-described selective conditions. In addition the stable transformation of plastids is of advantages because plastids are inherited maternally is most crops reducing or eliminating the risk of transgene flow through pollen. The transformation of the chloroplast genome is generally achieved by a process which has been schematically displayed in Klaus et al., 2004 [Nature Biotechnology 22 (2), 225-229]. Briefly the sequences to be transformed are cloned together with a selectable marker gene between flanking sequences homologous to the chloroplast genome. These homologous flanking sequences direct site specific integration into the plastome. Plastidal transformation has been described for many different plant species and an overview is given in Bock (2001) Transgenic plastids in basic research and plant biotechnology. J Mol Biol. 2001 Sep. 21; 312 (3):425-38 or Maliga, P (2003) Progress towards commercialization of plastid transformation technology. Trends Biotechnol. 21, 20-28. Further biotechnological progress has recently been reported in form of marker free plastid transformants, which can be produced by a transient co-integrated maker gene (Klaus et al., 2004, Nature Biotechnology 22(2), 225-229). The genetically modified plant cells can be regenerated via all methods with which the skilled worker is familiar. Suitable methods can be found in the abovementioned publications by S.D. Kung and R. Wu, Potrykus or Höfgen and Willmitzer.

Generally after transformation, plant cells or cell groupings are selected for the presence of one or more markers which are encoded by plant-expressible genes co-transferred with the gene of interest, following which the transformed material is regenerated into a whole plant. To select transformed plants, the plant material obtained in the transformation is, as a rule, subjected to selective conditions so that transformed plants can be distinguished from untransformed plants. For example, the seeds obtained in the above-described manner can be planted and, after an initial growing period, subjected to a suitable selection by spraying. A further possibility consists in growing the seeds, if appropriate after sterilization, on agar plates using a suitable selection agent so that only the transformed seeds can grow into plants. Alternatively, the transformed plants are screened for the presence of a selectable marker such as the ones described above.

Following DNA transfer and regeneration, putatively transformed plants may also be evaluated, for instance using Southern analysis, for the presence of the gene of interest, copy number and/or genomic organisation. Alternatively or additionally, expression levels of the newly introduced DNA may be monitored using Northern and/or Western analysis, both techniques being well known to persons having ordinary skill in the art.

The generated transformed plants may be propagated by a variety of means, such as by clonal propagation or classical breeding techniques. For example, a first generation (or T1) transformed plant may be selfed and homozygous second-generation (or T2) transformants selected, and the T2 plants may then further be propagated through classical breeding techniques. The generated transformed organisms may take a variety of forms. For example, they may be chimeras of transformed cells and non-transformed cells; clonal transformants (e.g., all cells transformed to contain the expression cassette); grafts of transformed and untransformed tissues (e.g., in plants, a transformed rootstock grafted to an untransformed scion).

Preferably, the expression of the nucleic acid in the plant results in the plant's increased tolerance to CESA-inhibiting herbicide as compared to a wild type variety of the plant.

In another embodiment, the invention refers to a plant, comprising a plant cell according to the present invention, wherein expression of the nucleic acid in the plant results in the plant's increased resistance to CESA-inhibiting herbicide as compared to a wild type variety of the plant.

The plants described herein can be either transgenic crop plants or non-transgenic plants.

In addition to the general definition, give SUPRA, “transgenic”, “transgene” or “recombinant” means with regard to, for example, a nucleic acid sequence, an expression cassette, gene construct or a vector comprising the nucleic acid sequence or an organism transformed with the nucleic acid sequences, expression cassettes or vectors according to the invention, all those constructions brought about by recombinant methods in which either

-   (a) the nucleic acid sequences encoding proteins useful in the     methods of the invention, or -   (b) genetic control sequence(s) which is operably linked with the     nucleic acid sequence according to the invention, for example a     promoter, or -   (c) a) and b)     are not located in their natural genetic environment or have been     modified by recombinant methods, it being possible for the     modification to take the form of, for example, a substitution,     addition, deletion, inversion or insertion of one or more nucleotide     residues in order to allow for the expression of the wildtype or     mutated CESA of the present invention. The natural genetic     environment is understood as meaning the natural genomic or     chromosomal locus in the original plant or the presence in a genomic     library. In the case of a genomic library, the natural genetic     environment of the nucleic acid sequence is preferably retained, at     least in part. The environment flanks the nucleic acid sequence at     least on one side and has a sequence length of at least 50 bp,     preferably at least 500 bp, especially preferably at least 1000 bp,     most preferably at least 5000 bp. A naturally occurring expression     cassette—for example the naturally occurring combination of the     natural promoter of the nucleic acid sequences with the     corresponding nucleic acid sequence encoding a polypeptide useful in     the methods of the present invention, as defined above—becomes a     transgenic expression cassette when this expression cassette is     modified by non-natural, synthetic (“artificial”) methods such as,     for example, mutagenic treatment. Suitable methods are described,     for example, in U.S. Pat. No. 5,565,350 or WO 00/15815.

A transgenic plant for the purposes of the invention is thus understood as meaning, as above, that the nucleic acids of the invention are not at their natural locus in the genome of said plant, it being possible for the nucleic acids to be expressed homologously or heterologously. However, as mentioned, transgenic also means that, while the nucleic acids according to the invention or used in the inventive method are at their natural position in the genome of a plant, the sequence has been modified with regard to the natural sequence, and/or that the regulatory sequences of the natural sequences have been modified. Transgenic is preferably understood as meaning the expression of the nucleic acids according to the invention at an unnatural locus in the genome, i.e. homologous or, preferably, heterologous expression of the nucleic acids takes place. Preferred transgenic plants are mentioned herein. Furthermore, the term “transgenic” refers to any plant, plant cell, callus, plant tissue, or plant part, that contains all or part of at least one recombinant polynucleotide. In many cases, all or part of the recombinant polynucleotide is stably integrated into a chromosome or stable extra-chromosomal element, so that it is passed on to successive generations. For the purposes of the invention, the term “recombinant polynucleotide” refers to a polynucleotide that has been altered, rearranged, or modified by genetic engineering. Examples include any cloned polynucleotide, or polynucleotides, that are linked or joined to heterologous sequences. The term “recombinant” does not refer to alterations of polynucleotides that result from naturally occurring events, such as spontaneous mutations, or from non-spontaneous mutagenesis followed by selective breeding.

Plants containing mutations arising due to non-spontaneous mutagenesis and selective breeding are referred to herein as non-transgenic plants and are included in the present invention. In embodiments wherein the plant is transgenic and comprises multiple wildtype or mutated CESA nucleic acids, the nucleic acids can be derived from different genomes or from the same genome. Alternatively, in embodiments wherein the plant is non-transgenic and comprises multiple wildtype or mutated CESA nucleic acids, the nucleic acids are located on different genomes or on the same genome.

In certain embodiments, the present invention involves herbicide-resistant plants that are produced by mutation breeding. Such plants comprise a polynucleotide encoding a wildtype or mutated CESA and are tolerant to one or more CESA-inhibiting herbicides. Such methods can involve, for example, exposing the plants or seeds to a mutagen, particularly a chemical mutagen such as, for example, ethyl methanesulfonate (EMS) and selecting for plants that have enhanced tolerance to at least one or more CESA-inhibiting herbicide [see Example 1].

However, the present invention is not limited to herbicide-tolerant plants that are produced by a mutagenesis method involving the chemical mutagen EMS. Any mutagenesis method known in the art may be used to produce the herbicide-resistant plants of the present invention. Such mutagenesis methods can involve, for example, the use of any one or more of the following mutagens: radiation, such as X-rays, Gamma rays (e.g., cobalt 60 or cesium 137), neutrons, (e.g., product of nuclear fission by uranium 235 in an atomic reactor), Beta radiation (e.g., emitted from radioisotopes such as phosphorus 32 or carbon 14), and ultraviolet radiation (preferably from 250 to 290 nm), and chemical mutagens such as base analogues (e.g., 5-bromo-uracil), related compounds (e.g., 8-ethoxy caffeine), antibiotics (e.g., streptonigrin), alkylating agents (e.g., sulfur mustards, nitrogen mustards, epoxides, ethylenamines, sulfates, sulfonates, sulfones, lactones), azide, hydroxylamine, nitrous acid, or acridines. Herbicide-resistant plants can also be produced by using tissue culture methods to select for plant cells comprising herbicide-resistance mutations and then regenerating herbicide-resistant plants therefrom. See, for example, U.S. Pat. Nos. 5,773,702 and 5,859,348, both of which are herein incorporated in their entirety by reference. Further details of mutation breeding can be found in “Principals of Cultivar Development” Fehr, 1993 Macmillan Publishing Company the disclosure of which is incorporated herein by reference

The plant of the present invention comprises at least one wildtype or mutated CESA nucleic acid or over-expressed wild-type CESA nucleic acid, and has increased tolerance to a CESA-inhibiting herbicide as compared to a wild-type variety of the plant. It is possible for the plants of the present invention to have multiple wildtype or mutated CESA nucleic acids from different genomes since these plants can contain more than one genome. For example, a plant contains two genomes, usually referred to as the A and B genomes. Because CESA is a required metabolic enzyme, it is assumed that each genome has at least one gene coding for the CESA enzyme (i.e. at least one CESA gene). As used herein, the term “CESA gene locus” refers to the position of a CESA gene on a genome, and the terms “CESA gene” and “CESA nucleic acid” refer to a nucleic acid encoding the CESA enzyme. The CESA nucleic acid on each genome differs in its nucleotide sequence from a CESA nucleic acid on another genome. One of skill in the art can determine the genome of origin of each CESA nucleic acid through genetic crossing and/or either sequencing methods or exonuclease digestion methods known to those of skill in the art.

The present invention includes plants comprising one, two, three, or more wildtype or mutated CESA alleles, wherein the plant has increased tolerance to a CESA-inhibiting herbicide as compared to a wild-type variety of the plant. The wildtype or mutated CESA alleles can comprise a nucleotide sequence selected from the group consisting of a polynucleotide as defined in SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a variant or derivative thereof, a polynucleotide encoding a polypeptide as defined in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, or 64, or a variant or derivative, homologue, orthologue, paralogue thereof, a polynucleotide comprising at least 60 consecutive nucleotides of any of the aforementioned polynucleotides; and a polynucleotide complementary to any of the aforementioned polynucleotides.

“Alleles” or “allelic variants” are alternative forms of a given gene, located at the same chromosomal position. Allelic variants encompass Single Nucleotide Polymorphisms (SNPs), as well as Small Insertion/Deletion Polymorphisms (INDELs). The size of INDELs is usually less than 100 bp. SNPs and INDELs form the largest set of sequence variants in naturally occurring polymorphic strains of most organisms

The term “variety” refers to a group of plants within a species defined by the sharing of a common set of characteristics or traits accepted by those skilled in the art as sufficient to distinguish one cultivar or variety from another cultivar or variety. There is no implication in either term that all plants of any given cultivar or variety will be genetically identical at either the whole gene or molecular level or that any given plant will be homozygous at all loci. A cultivar or variety is considered “true breeding” for a particular trait if, when the true-breeding cultivar or variety is self-pollinated, all of the progeny contain the trait. The terms “breeding line” or “line” refer to a group of plants within a cultivar defined by the sharing of a common set of characteristics or traits accepted by those skilled in the art as sufficient to distinguish one breeding line or line from another breeding line or line. There is no implication in either term that all plants of any given breeding line or line will be genetically identical at either the whole gene or molecular level or that any given plant will be homozygous at all loci. A breeding line or line is considered “true breeding” for a particular trait if, when the true-breeding line or breeding line is self-pollinated, all of the progeny contain the trait. In the present invention, the trait arises from a mutation in a CESA gene of the plant or seed.

The herbicide-resistant plants of the invention that comprise polynucleotides encoding wildtype or mutated CESA polypeptides also find use in methods for increasing the herbicide-resistance of a plant through conventional plant breeding involving sexual reproduction. The methods comprise crossing a first plant that is a herbicide-resistant plant of the invention to a second plant that may or may not be resistant to the same herbicide or herbicides as the first plant or may be resistant to different herbicide or herbicides than the first plant. The second plant can be any plant that is capable of producing viable progeny plants (i.e., seeds) when crossed with the first plant. Typically, but not necessarily, the first and second plants are of the same species. The methods can optionally involve selecting for progeny plants that comprise the wildtype or mutated CESA polypeptides of the first plant and the herbicide resistance characteristics of the second plant. The progeny plants produced by this method of the present invention have increased resistance to a herbicide when compared to either the first or second plant or both. When the first and second plants are resistant to different herbicides, the progeny plants will have the combined herbicide tolerance characteristics of the first and second plants. The methods of the invention can further involve one or more generations of backcrossing the progeny plants of the first cross to a plant of the same line or genotype as either the first or second plant. Alternatively, the progeny of the first cross or any subsequent cross can be crossed to a third plant that is of a different line or genotype than either the first or second plant. The present invention also provides plants, plant organs, plant tissues, plant cells, seeds, and non-human host cells that are transformed with the at least one polynucleotide molecule, expression cassette, or transformation vector of the invention. Such transformed plants, plant organs, plant tissues, plant cells, seeds, and non-human host cells have enhanced tolerance or resistance to at least one herbicide, at levels of the herbicide that kill or inhibit the growth of an untransformed plant, plant tissue, plant cell, or non-human host cell, respectively. Preferably, the transformed plants, plant tissues, plant cells, and seeds of the invention are Arabidopsis thaliana and crop plants.

It is to be understood that the plant of the present invention can comprise a wild type CESA nucleic acid in addition to a mutated CESA nucleic acid. It is contemplated that the CESA-inhibiting herbicide tolerant lines may contain a mutation in only one of multiple CESA isoenzymes. Therefore, the present invention includes a plant comprising one or more mutated CESA nucleic acids in addition to one or more wild type CESA nucleic acids.

In another embodiment, the invention refers to a seed produced by a transgenic plant comprising a plant cell of the present invention, wherein the seed is true breeding for an increased resistance to a CESA-inhibiting herbicide as compared to a wild type variety of the seed.

In other aspects, CESA-inhibiting herbicides-tolerant plants of the present invention can be employed as CESA-inhibiting herbicides-tolerance trait donor lines for development, as by traditional plant breeding, to produce other varietal and/or hybrid crops containing such trait or traits. All such resulting variety or hybrids crops, containing the ancestral CESA-inhibiting herbicides-tolerance trait or traits can be referred to herein as progeny or descendant of the ancestral, CESA-inhibiting herbicides-tolerant line(s).

In other embodiments, the present invention provides a method for producing a CESA-inhibiting herbicides-tolerant plant. The method comprises: crossing a first CESA-inhibiting herbicides-tolerant plant with a second plant to produce a CESA-inhibiting herbicides-tolerant progeny plant, wherein the first plant and the progeny plant comprise in at least some of their cells a polynucleotide operably linked to a promoter operable in plant cells, the recombinant polynucleotide being effective in the cells of the first plant to express a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

In some embodiments, traditional plant breeding is employed whereby the CESA-inhibiting herbicides-tolerant trait is introduced in the progeny plant resulting therefrom. In one embodiment, the present invention provides a method for producing a CESA-inhibiting herbicides-tolerant progeny plant, the method comprising: crossing a parent plant with a CESA-inhibiting herbicides-tolerant plant to introduce the CESA-inhibiting herbicides-tolerance characteristics of the CESA-inhibiting herbicides-tolerant plant into the germplasm of the progeny plant, wherein the progeny plant has increased tolerance to the CESA-inhibiting herbicides relative to the parent plant. In other embodiments, the method further comprises the step of introgressing the CESA-inhibiting herbicides-tolerance characteristics through traditional plant breeding techniques to obtain a descendent plant having the CESA-inhibiting herbicides-tolerance characteristics.

In other aspects, plants of the invention include those plants which, in addition to being CESA-inhibiting herbicides-tolerant, have been subjected to further genetic modifications by breeding, mutagenesis or genetic engineering, e.g. have been rendered tolerant to applications of specific other classes of herbicides, such as AHAS inhibitors; auxinic herbicides; bleaching herbicides such as hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors or phytoene desaturase (PDS) inhibitors; EPSPS inhibitors such as glyphosate; glutamine synthetase (GS) inhibitors such as glufosinate; lipid biosynthesis inhibitors such as acetyl CoA carboxylase (ACCase) inhibitors; or oxynil {i.e. bromoxynil or ioxynil) herbicides as a result of conventional methods of breeding or genetic engineering, Thus, CESA-inhibiting herbicides-tolerant plants of the invention can be made resistant to multiple classes of herbicides through multiple genetic modifications, such as resistance to both glyphosate and glufosinate or to both glyphosate and a herbicide from another class such as HPPD inhibitors, AHAS inhibitors, or ACCase inhibitors. These herbicide resistance technologies are, for example, described in Pest Management Science (at volume, year, page): 61, 2005, 246; 61, 2005, 258; 61, 2005, 277; 61, 2005, 269; 61, 2005, 286; 64, 2008, 326; 64, 2008, 332; Weed Science 57, 2009, 108; Australian Journal of Agricultural Research 58, 2007, 708; Science 316, 2007, 1185; and references quoted therein. For example, CESA-inhibiting herbicides-tolerant plants of the invention, in some embodiments, may be tolerant to ACCase inhibitors, such as “dims” {e.g., cycloxydim, sethoxydim, clethodim, or tepraloxydim), “fops” {e.g. clodinafop, diclofop, fluazifop, haloxyfop, or quizalofop), and “dens” (such as pinoxaden); to auxinic herbicides, such as dicamba; to EPSPS inhibitors, such as glyphosate; to other CESA inhibitors; and to GS inhibitors, such as glufosinate.

In addition to these classes of inhibitors, CESA-inhibiting herbicides-tolerant plants of the invention may also be tolerant to herbicides having other modes of action, for example, chlorophyll/carotenoid pigment inhibitors, cell membrane disrupters, photosynthesis inhibitors, cell division inhibitors, root inhibitors, shoot inhibitors, and combinations thereof.

Such tolerance traits may be expressed, e.g.: as mutant or wildtype HPPD proteins, as mutant AHASL proteins, mutant ACCase proteins, mutant EPSPS proteins, or mutant glutamine synthetase proteins; or as mutant native, inbred, or transgenic aryloxyalkanoate dioxygenase (AAD or DHT), haloarylnitrilase (BXN), 2,2-dichloropropionic acid dehalogenase (DEH), glyphosate-N-acetyltransferase (GAT), glyphosate decarboxylase (GDC), glyphosate oxidoreductase (GOX), glutathione-S-transferase (GST), phosphinothricin acetyltransferase (PAT or bar), or CYP450s proteins having an herbicide-degrading activity. CESA-inhibiting herbicides-tolerant plants hereof can also be stacked with other traits including, but not limited to, pesticidal traits such as Bt Cry and other proteins having pesticidal activity toward coleopteran, lepidopteran, nematode, or other pests; nutrition or nutraceutical traits such as modified oil content or oil profile traits, high protein or high amino acid concentration traits, and other trait types known in the art.

Furthermore, in other embodiments, CESA-inhibiting herbicides-tolerant plants are also covered which are, by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such characteristics, rendered able to synthesize one or more insecticidal proteins, especially those known from the bacterial genus Bacillus, particularly from Bacillus thuringiensis, such as [delta]-endotoxins, e.g. CryIA(b), CryIA(c), CryIF, CryIF(a2), CryIIA(b), CryIIIA, CryIIIB(bI) or Cry9c; vegetative insecticidal proteins (VIP), e.g. VIP1, VIP2, VIP3 or VIP3A; insecticidal proteins of bacteria colonizing nematodes, e.g. Photorhabdus spp. or Xenorhabdus spp.; toxins produced by animals, such as scorpion toxins, arachnid toxins, wasp toxins, or other insect-specific neurotoxins; toxins produced by fungi, such streptomycete toxins; plant lectins, such as pea or barley lectins; agglutinins; proteinase inhibitors, such as trypsin inhibitors, serine protease inhibitors, patatin, cystatin or papain inhibitors; ribosome-inactivating proteins (RIP), such as ricin, maize-RIP, abrin, luffin, saporin or bryodin; steroid metabolism enzymes, such as 3-hydroxy-steroid oxidase, ecdysteroid-IDP-glycosyl-transferase, cholesterol oxidases, ecdysone inhibitors or HMG-CoA-reductase; ion channel blockers, such as blockers of sodium or calcium channels; juvenile hormone esterase; diuretic hormone receptors (helicokinin receptors); stilben synthase, bibenzyl synthase, chitinases or glucanases. In the context of the present invention these insecticidal proteins or toxins are to be understood expressly also as pre-toxins, hybrid proteins, truncated or otherwise modified proteins. Hybrid proteins are characterized by a new combination of protein domains, (see, e.g. WO 02/015701). Further examples of such toxins or genetically modified plants capable of synthesizing such toxins are disclosed, e.g., in EP-A 374 753, WO 93/007278, WO 95/34656, EP-A 427 529, EP-A 451 878, WO 03/18810 and WO 03/52073. The methods for producing such genetically modified plants are generally known to the person skilled in the art and are described, e.g. in the publications mentioned above. These insecticidal proteins contained in the genetically modified plants impart to the plants producing these proteins tolerance to harmful pests from all taxonomic groups of arthropods, especially to beetles (Coeloptera), two-winged insects (Diptera), and moths (Lepidoptera) and to nematodes (Nematoda).

In some embodiments, expression of one or more protein toxins (e.g., insecticidal proteins) in the CESA-inhibiting herbicides-tolerant plants is effective for controlling organisms that include, for example, members of the classes and orders: Coleoptera such as the American bean weevil Acanthoscelides obtectus; the leaf beetle Agelastica alni; click beetles (Agriotes lineatus, Agriotes obscurus, Agriotes bicolor); the grain beetle Ahasverus advena; the summer schafer Amphimallon solstitialis; the furniture beetle Anobium punctatum; Anthonomus spp. (weevils); the Pygmy mangold beetle Atomaria linearis; carpet beetles (Anthrenus spp., Attagenus spp.); the cowpea weevil Callosobruchus maculates; the fried fruit beetle Carpophilus hemipterus; the cabbage seedpod weevil Ceutorhynchus assimilis; the rape winter stem weevil Ceutorhynchus picitarsis; the wireworms Conoderus vespertinus and Conoderus falli; the banana weevil Cosmopolites sordidus; the New Zealand grass grub Costelytra zealandica; the June beetle Cotinis nitida; the sunflower stem weevil Cylindrocopturus adspersus; the larder beetle Dermestes lardarius; the corn rootworms Diabrotica virgifera, Diabrotica virgifera virgifera, and Diabrotica barberi; the Mexican bean beetle Epilachna varivestis; the old house borer Hylotropes bajulus; the lucerne weevil Hypera postica; the shiny spider beetle Gibbium psylloides; the cigarette beetle Lasioderma serricorne; the Colorado potato beetle Leptinotarsa decemlineata; Lyctus beetles {Lyctus spp., the pollen beetle Meligethes aeneus; the common cockshafer Melolontha melolontha; the American spider beetle Mezium americanum; the golden spider beetle Niptus hololeucs; the grain beetles Oryzaephilus surinamensis and Oryzaephilus Mercator; the black vine weevil Otiorhynchus sulcatus; the mustard beetle Phaedon cochleariae, the crucifer flea beetle Phyllotreta cruciferae; the striped flea beetle Phyllotreta striolata; the cabbage steam flea beetle Psylliodes chrysocephala; Ptinus spp. (spider beetles); the lesser grain borer Rhizopertha dominica; the pea and been weevil Sitona lineatus; the rice and granary beetles Sitophilus oryzae and Sitophilus granaries; the red sunflower seed weevil Smicronyx fulvus; the drugstore beetle Stegobium paniceum; the yellow mealworm beetle Tenebrio molitor, the flour beetles Tribolium castaneum and Tribolium confusum; warehouse and cabinet beetles {Trogoderma spp.); the sunflower beetle Zygogramma exclamationis; Dermaptera (earwigs) such as the European earwig Forficula auricularia and the striped earwig Labidura riparia; Dictyoptera such as the oriental cockroach Blatta orientalis; the greenhouse millipede Oxidus gracilis; the beet fly Pegomyia betae; the frit fly Oscinella frit; fruitflies (Dacus spp., Drosophila spp.); Isoptera (termites) including species from the families Hodotermitidae, Kalotermitidae, Mastotermitidae, Rhinotermitidae, Serritermitidae, Termitidae, Termopsidae; the tarnished plant bug Lygus lineolaris; the black bean aphid Aphis fabae; the cotton or melon aphid Aphis gossypii; the green apple aphid Aphis pomi; the citrus spiny whitefly Aleurocanthus spiniferus; the sweet potato whitefly Bemesia tabaci; the cabbage aphid Brevicoryne brassicae; the pear psylla Cacopsylla pyricola; the currant aphid Cryptomyzus ribis; the grape phylloxera Daktulosphaira vitifoliae; the citrus psylla Diaphorina citri; the potato leafhopper Empoasca fabae; the bean leafhopper Empoasca Solana; the vine leafhopper Empoasca vitis; the woolly aphid Eriosoma lanigerum; the European fruit scale Eulecanium corni; the mealy plum aphid Hyalopterus arundinis; the small brown planthopper Laodelphax striatellus; the potato aphid Macrosiphum euphorbiae; the green peach aphid Myzus persicae; the green rice leafhopper Nephotettix cinticeps; the brown planthopper Nilaparvata lugens; the hop aphid Phorodon humuli; the bird-cherry aphid Rhopalosiphum padi; the grain aphid Sitobion avenae; Lepidoptera such as Adoxophyes orana (summer fruit tortrix moth); Archips podana (fruit tree tortrix moth); Bucculatrix pyrivorella (pear leafminer); Bucculatrix thurberiella (cotton leaf perforator); Bupalus piniarius (pine looper); Carpocapsa pomonella (codling moth); Chilo suppressalis (striped rice borer); Choristoneura fumiferana (eastern spruce budworm); Cochylis hospes (banded sunflower moth); Diatraea grandiosella (southwestern corn borer); Eupoecilia ambiguella (European grape berry moth); Helicoverpa armigera (cotton bollworm); Helicoverpa zea (cotton bollworm); Heliothis vires cens (tobacco budworm), Homeosoma electellum (sunflower moth); Homona magnanima (oriental tea tree tortrix moth); Lithocolletis blancardella (spotted tentiform leafminer); Lymantria dispar (gypsy moth); Malacosoma neustria (tent caterpillar); Mamestra brassicae (cabbage armyworm); Mamestra configurata (Bertha armyworm); Operophtera brumata (winter moth); Ostrinia nubilalis (European corn borer), Panolis flammea (pine beauty moth), Phyllocnistis citrella (citrus leafminer); Pieris brassicae (cabbage white butterfly); Rachiplusia ni (soybean looper); Spodoptera exigua (beet armywonn); Spodoptera littoralis (cotton leafworm); Sylepta derogata (cotton leaf roller); Trichoplusia ni (cabbage looper); Orthoptera such as the common cricket Acheta domesticus, tree locusts (Anacridium spp.), the migratory locust Locusta migratoria, the twostriped grasshopper Melanoplus bivittatus, the differential grasshopper Melanoplus differ entialis, the redlegged grasshopper Melanoplus femurrubrum, the migratory grasshopper Melanoplus sanguinipes, the northern mole cricket Neocurtilla hexadectyla, the red locust Nomadacris septemfasciata, the shortwinged mole cricket Scapteriscus abbreviatus, the southern mole cricket Scapteriscus borellii, the tawny mole cricket Scapteriscus vicinus, and the desert locust Schistocerca gregaria; Symphyla such as the garden symphylan Scutigerella immaculata; Thysanoptera such as the tobacco thrips Frankliniella fusca, the flower thrips Frankliniella intonsa, the western flower thrips Frankliniella occidentalism the cotton bud thrips Frankliniella schultzei, the banded greenhouse thrips Hercinothrips femoralis, the soybean thrips Neohydatothrips variabilis, Kelly's citrus thrips Pezothrips kellyanus, the avocado thrips Scirtothrips perseae, the melon thrips Thrips palmi, and the onion thrips Thrips tabaci; and the like, and combinations comprising one or more of the foregoing organisms.

In some embodiments, expression of one or more protein toxins (e.g., insecticidal proteins) in the CESA-inhibiting herbicides-tolerant plants is effective for controlling flea beetles, i.e. members of the flea beetle tribe of family Chrysomelidae, preferably against Phyllotreta spp., such as Phyllotreta cruciferae and/or Phyllotreta triolata. In other embodiments, expression of one or more protein toxins {e.g., insecticidal proteins) in the CESA-inhibiting herbicides-tolerant plants is effective for controlling cabbage seedpod weevil, the Bertha armyworm, Lygus bugs, or the diamondback moth.

Furthermore, in one embodiment, CESA-inhibiting herbicides-tolerant plants are also covered which are, e.g. by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such traits, rendered able to synthesize one or more proteins to increase the resistance or tolerance of those plants to bacterial, viral or fungal pathogens. The methods for producing such genetically modified plants are generally known to the person skilled in the art.

Furthermore, in another embodiment, CESA-inhibiting herbicides-tolerant plants are also covered which are, e.g. by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such traits, rendered able to synthesize one or more proteins to increase the productivity (e.g. oil content), tolerance to drought, salinity or other growth-limiting environmental factors or tolerance to pests and fungal, bacterial or viral pathogens of those plants.

Furthermore, in other embodiments, CESA-inhibiting herbicides-tolerant plants are also covered which are, e.g. by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such traits, altered to contain a modified amount of one or more substances or new substances, for example, to improve human or animal nutrition, e.g. oil crops that produce health-promoting long-chain omega-3 fatty acids or unsaturated omega-9 fatty acids (e.g. Nexera® rape, Dow Agro Sciences, Canada).

Furthermore, in some embodiments, CESA-inhibiting herbicides-tolerant plants are also covered which are, e.g. by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such traits, altered to contain increased amounts of vitamins and/or minerals, and/or improved profiles of nutraceutical compounds.

In one embodiment, CESA-inhibiting herbicides-tolerant plants of the present invention, relative to a wild-type plant, comprise an increased amount of, or an improved profile of, a compound selected from the group consisting of: glucosinolates (e.g., glucoraphanin (4-methylsulfinylbutyl-glucosinolate), sulforaphane, 3-indolylmethyl-glucosinolate(glucobrassicin), 1-methoxy-3-indolylmethyl-glucosinolate (neoglucobrassicin)); phenolics (e.g., flavonoids (e.g., quercetin, kaempferol), hydroxycinnamoyl derivatives (e.g., 1,2,2′-trisinapoylgentiobiose, 1,2-diferuloylgentiobiose, 1,2′-disinapoyl-2-feruloylgentiobiose, 3-0-caffeoyl-quinic (neochlorogenic acid)); and vitamins and minerals (e.g., vitamin C, vitamin E, carotene, folic acid, niacin, riboflavin, thiamine, calcium, iron, magnesium, potassium, selenium, and zinc).

In another embodiment, CESA-inhibiting herbicides-tolerant plants of the present invention, relative to a wild-type plant, comprise an increased amount of, or an improved profile of, a compound selected from the group consisting of: progoitrin; isothiocyanates; indoles (products of glucosinolate hydrolysis); glutathione; carotenoids such as beta-carotene, lycopene, and the xanthophyll carotenoids such as lutein and zeaxanthin; phenolics comprising the flavonoids such as the flavonols (e.g. quercetin, rutin), the flavans/tannins (such as the procyanidins comprising coumarin, proanthocyanidins, catechins, and anthocyanins); flavones; phytoestrogens such as coumestans, lignans, resveratrol, isoflavones e.g. genistein, daidzein, and glycitein; resorcyclic acid lactones; organosulphur compounds; phytosterols; terpenoids such as carnosol, rosmarinic acid, glycyrrhizin and saponins; chlorophyll; chlorphyllin, sugars, anthocyanins, and vanilla.

In other embodiments, CESA-inhibiting herbicides-tolerant plants of the present invention, relative to a wild-type plant, comprise an increased amount of, or an improved profile of, a compound selected from the group consisting of: vincristine, vinblastine, taxanes (e.g., taxol (paclitaxel), baccatin III, 10-desacetylbaccatin III, 10-desacetyl taxol, xylosyl taxol, 7-epitaxol, 7-epibaccatin III, 10-desacetylcephalomannine, 7-epicephalomannine, taxotere, cephalomannine, xylosyl cephalomannine, taxagifine, 8-benxoyloxy taxagifine, 9-acetyloxy taxusin, 9-hydroxy taxusin, taiwanxam, taxane Ia, taxane Ib, taxane Ic, taxane Id, GMP paclitaxel, 9-dihydro 13-acetylbaccatin III, 10-desacetyl-7-epitaxol, tetrahydrocannabinol (THC), cannabidiol (CBD), genistein, diadzein, codeine, morphine, quinine, shikonin, ajmalacine, serpentine, and the like.

In other aspects, a method for treating a plant of the present invention is provided.

In some embodiments, the method comprises contacting the plant with an agronomically acceptable composition. In one embodiment, the agronomically acceptable composition comprises a CESA inhibiting herbicide A. I, such as an azine as described herein.

In another aspect, the present invention provides a method for preparing a descendent seed. The method comprises planting a seed of or capable of producing a plant of the present invention. In one embodiment, the method further comprises growing a descendent plant from the seed; and harvesting a descendant seed from the descendent plant. In other embodiments, the method further comprises applying a CESA-inhibiting herbicides herbicidal composition to the descendent plant.

In another embodiment, the invention refers to harvestable parts of the plant according to the present invention. Preferably, the harvestable parts comprise the CESA nucleic acid or CESA protein of the present invention. The harvestable parts may be seeds, roots, leaves and/or flowers comprising the CESA nucleic acid or CESA protein or parts thereof. Preferred parts of soy plants are soy beans comprising the CESA nucleic acid or CESA protein.

In another embodiment, the invention refers to products derived from a plant according to the present invention, parts thereof or harvestable parts thereof. A preferred plant product is fodder, seed meal, oil, or seed-treatment-coated seeds. Preferably, the meal and/or oil comprise the CESA nucleic acids or CESA proteins.

In another embodiment, the invention refers to a method for the production of a product, which method comprises

-   a) growing the plants of the invention or obtainable by the methods     of invention and -   b) producing said product from or by the plants of the invention     and/or parts, e.g. seeds, of these plants.

In a further embodiment the method comprises the steps

a) growing the plants of the invention,

b) removing the harvestable parts as defined above from the plants and

c) producing said product from or by the harvestable parts of the invention.

The product may be produced at the site where the plant has been grown, the plants and/or parts thereof may be removed from the site where the plants have been grown to produce the product. Typically, the plant is grown, the desired harvestable parts are removed from the plant, if feasible in repeated cycles, and the product made from the harvestable parts of the plant. The step of growing the plant may be performed only once each time the methods of the invention is performed, while allowing repeated times the steps of product production e.g. by repeated removal of harvestable parts of the plants of the invention and if necessary further processing of these parts to arrive at the product. It is also possible that the step of growing the plants of the invention is repeated and plants or harvestable parts are stored until the production of the product is then performed once for the accumulated plants or plant parts. Also, the steps of growing the plants and producing the product may be performed with an overlap in time, even simultaneously to a large extend or sequentially. Generally the plants are grown for some time before the product is produced.

In one embodiment the products produced by said methods of the invention are plant products such as, but not limited to, a foodstuff, feedstuff, a food supplement, feed supplement, fiber, cosmetic and/or pharmaceutical. Foodstuffs are regarded as compositions used for nutrition and/or for supplementing nutrition. Animal feedstuffs and animal feed supplements, in particular, are regarded as foodstuffs.

In another embodiment the inventive methods for the production are used to make agricultural products such as, but not limited to, plant extracts, proteins, amino acids, carbohydrates, fats, oils, polymers, vitamins, and the like.

It is possible that a plant product consists of one or more agricultural products to a large extent.

Herbicides

As described above, the present invention provides nucleic acids, polypeptides, conferring tolerance of plants to compounds/herbicides interfering or inhibiting cell wall (cellulose) biosynthesis by interfering with the activity of cellulose synthase (“CESA-inhibiting herbicides”), also known to the person skilled in the art as Cellulose Biosynthesis Inhibitors (CBI).

Examples of herbicides which can be used according to the present invention, i.e. to which the plants according to the present invention are tolerant/resistant to, are compounds known to the skilled artisan as azines. Examples of Azines are described in detail in the following patent applications depicted in the following Table 1, which are incorporated by reference in its entirety.

TABLE 1 Publication or Application number/Internal No.: Structural Formula reference 1

WO 2014/064094 PF74283 2

WO 2015/007711 PF75365 3

PCT/EP2015/056711 PF76068 4

EP 14163356.0 PF76069 5

EP 14163742.1 PF76635 6

EP 14163743.9 PF76636 7

EP 14165565.4 PF76857 8

EP 14165624.9 PF76888 9

EP 14164431.0 PF76890 10

EP 14164434.4 PF76930 11

EP 14164433.6 PF77027 12

Indaziflam 13

Triazofenamid

Examples of preferred CESA inhibiting herbicides from the group of so-called azines which can be used according to the present invention are compounds having the Formula (I), known to the skilled artisan as azines.

wherein

-   A is phenyl, which is substituted by two to five substituents     selected from the group consisting of halogen, CN, NO₂, C₁-C₆-alkyl,     C₁-C₆-haloalkyl, C₂-C₆-alkenyl, C₂-C₆-haloalkenyl, C₂-C₆-alkynyl,     C₁-C₆-haloalkynyl, OH, C₁-C₆-alkoxy, C₁-C₆-alkylthio,     (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl, amino,     (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)carbonyl,     (C₁-C₆-alkoxy)carbonyl; -   R¹ H, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy-C₁-C₆-alkyl,     C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl, (C₁-C₆-alkoxy)carbonyl,     (C₁-C₆-alkyl)sulfonyl or phenylsulfonyl,     -   wherein the phenyl is unsubstituted or substituted by one to         five substituents selected from the group consisting of halogen,         CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl and C₁-C₆-alkoxy; -   R² H, halogen, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₂-C₆-alkenyl,     C₃-C₆-alkynyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl, OH,     C₁-C₆-alkoxy or C₁-C₆-alkoxy-C₁-C₆-alkyl; -   R³ H, halogen, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl or C₁-C₆-alkoxy; -   R⁴ H, halogen, CN, C₁-C₆-alkyl or C₁-C₆-haloalkyl; or -   R³ and R⁴ together with the carbon atom to which they are attached     form a moiety selected from the group consisting of carbonyl,     C₂-C₆-alkenyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl and three- to     six-membered heterocyclyl,     -   wherein the C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl, or three- to         six-membered heterocyclyl is unsubstituted or substituted by one         to three substituents selected from halogen, CN, C₁-C₆-alkyl and         C₁-C₆-alkoxy; and -   R⁵ H, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy-C₁-C₆-alkyl,     C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl, (C₁-C₆-alkoxy)carbonyl,     (C₁-C₆-alkyl)sulfonyl or phenylsulfonyl,     -   wherein the phenyl is unsubstituted or substituted by one to         five substituents selected from the group consisting of halogen,         CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl and C₁-C₆-alkoxy;         including their agriculturally acceptable salts or N-oxides.

Preferably the present invention provides azines of formula (I), wherein

-   -   A is 2-fluoro-phenyl, which is substituted by one to four         substituents selected from the group consisting of halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)carbonyl and (C₁-C₆-alkoxy)-carbonyl;     -   R¹ H, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl,         C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl,         (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl or phenylsulfonyl,         -   wherein the phenyl is unsubstituted or substituted by one to             five substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl and             C₁-C₆-alkoxy;     -   R² H, halogen, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₂-C₆-alkenyl,         C₃-C₆-alkynyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl, OH,         C₁-C₆-alkoxy or C₁-C₆-alkoxy-C₁-C₆-alkyl;     -   R³ H, halogen, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl or C₁-C₆-alkoxy;     -   R⁴ H, halogen, CN, C₁-C₆-alkyl or C₁-C₆-haloalkyl; or     -   R³ and R⁴ together with the carbon atom to which they are         attached form a moiety selected from the group consisting of         carbonyl, C₂-C₆-alkenyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl         and three- to six-membered heterocyclyl,         -   wherein the C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl or and             three- to six-membered heterocyclyl is unsubstituted or             substituted by one to three substituents selected from             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy; and     -   R⁵ H, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl,         C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl,         (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl or phenylsulfonyl,         -   wherein the phenyl is unsubstituted or substituted by one to             five substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl and             C₁-C₆-alkoxy;     -   including their agriculturally acceptable salts or N-oxides.

Useful for the present invention are also agrochemical compositions comprising at least one azines of formula (I) and auxiliaries customary for formulating crop protection agents.

The present invention also provides the use of azines of formula (I) as herbicides, i.e. for controlling harmful plants.

If the azines of formula (I) as described herein are capable of forming geometrical isomers, for example E/Z isomers, it is possible to use both, the pure isomers and mixtures thereof, in the compositions according to the invention.

If the azines of formula (I) as described herein have one or more centres of chirality and, as a consequence, are present as enantiomers or diastereomers, it is possible to use both, the pure enantiomers and diastereomers and their mixtures, in the compositions according to the invention.

If the azines of formula (I) as described herein have ionizable functional groups, they can also be employed in the form of their agriculturally acceptable salts. Suitable are, in general, the salts of those cations and the acid addition salts of those acids whose cations and anions, respectively, have no adverse effect on the activity of the active compounds.

Preferred cations are the ions of the alkali metals, preferably of lithium, sodium and potassium, of the alkaline earth metals, preferably of calcium and magnesium, and of the transition metals, preferably of manganese, copper, zinc and iron, further ammonium and substituted ammonium in which one to four hydrogen atoms are replaced by C₁-C₄-alkyl, hydroxy-C₁-C₄-alkyl, C₁-C₄-alkoxy-C₁-C₄-alkyl, hydroxy-C₁-C₄-alkoxy-C₁-C₄-alkyl, phenyl or benzyl, preferably ammonium, methylammonium, isopropylammonium, dimethylammonium, diisopropylammonium, trimethylammonium, heptylammonium, dodecylammonium, tetradecylammonium, tetramethylammonium, tetraethylammonium, tetrabutylammonium, 2-hydroxyethylammonium (olamine salt), 2-(2-hydroxyeth-1-oxy)eth-1-ylammonium (diglycolamine salt), di(2-hydroxyeth-1-yl)ammonium (diolamine salt), tris(2-hydroxyethyl)ammonium (trolamine salt), tris(2-hydroxypropyl)ammonium, benzyltrimethylammonium, benzyltriethylammonium, N,N,N-trimethylethanolammonium (choline salt), furthermore phosphonium ions, sulfonium ions, preferably tri(C₁-C₄-alkyl)sulfonium, such as trimethylsulfonium, and sulfoxonium ions, preferably tri(C₁-C₄-alkyl)sulfoxonium, and finally the salts of polybasic amines such as N,N-bis-(3-aminopropyl)methylamine and diethylenetriamine.

Anions of useful acid addition salts are primarily chloride, bromide, fluoride, iodide, hydrogensulfate, methylsulfate, sulfate, dihydrogenphosphate, hydrogenphosphate, nitrate, bicarbonate, carbonate, hexafluorosilicate, hexafluorophosphate, benzoate and also the anions of C₁-C₄-alkanoic acids, preferably formate, acetate, propionate and butyrate.

The organic moieties mentioned in the definition of the variables, e.g. R¹ to R⁵, are—like the term halogen—collective terms for individual enumerations of the individual group members. The term halogen denotes in each case fluorine, chlorine, bromine or iodine. All hydrocarbon chains, i.e. all alkyl, haloalkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, (alkyl)amino, di(alkyl)amino chains can be straight-chain or branched, the prefix C_(n)-C_(m) denoting in each case the possible number of carbon atoms in the group.

Examples of such meanings are:

-   -   C₁-C₄-alkyl: for example CH₃, C₂H₅, n-propyl, CH(CH₃)₂, n-butyl,         CH(CH₃)—C₂H₅, CH₂—CH(CH₃)₂ and C(CH₃)₃;     -   C₁-C₆-alkyl and also the C₁-C₆-alkyl moieties of         (C₁-C₆-alkyl)carbonyl, C₁-C₆-alkyloxy-C₁-C₆-alkyl: C₁-C₄-alkyl         as mentioned above, and also, for example, n-pentyl,         1-methylbutyl, 2-methylbutyl, 3-methylbutyl, 2,2-dimethylpropyl,         1-ethylpropyl, n-hexyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl,         1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl,         1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-dimethylbutyl,         2,2-dimethylbutyl, 2,3-dimethylbutyl, 3,3-dimethylbutyl,         1-ethylbutyl, 2-ethylbutyl, 1,1,2-trimethylpropyl,         1,2,2-trimethylpropyl, 1-ethyl-1-methylpropyl or         1-ethyl-2-methylpropyl, preferably methyl, ethyl, n-propyl,         1-methylethyl, n-butyl, 1,1-dimethylethyl, n-pentyl or n-hexyl;     -   C₁-C₄-haloalkyl: a C₁-C₄-alkyl radical as mentioned above which         is partially or fully substituted by fluorine, chlorine, bromine         and/or iodine, for example, chloromethyl, dichloromethyl,         trichloromethyl, fluoromethyl, difluoromethyl, trifluoromethyl,         chlorofluoromethyl, dichlorofluoromethyl, chlorodifluoromethyl,         bromomethyl, iodomethyl, 2-fluoroethyl, 2-chloroethyl,         2-bromoethyl, 2-iodoethyl, 2,2-difluoroethyl,         2,2,2-trifluoroethyl, 2-chloro-2-fluoroethyl,         2-chloro-2,2-difluoroethyl, 2,2-dichloro-2-fluoroethyl,         2,2,2-trichloroethyl, pentafluoroethyl, 2-fluoropropyl,         3-fluoropropyl, 2,2-difluoropropyl, 2,3-difluoropropyl,         2-chloropropyl, 3-chloropropyl, 2,3-dichloropropyl,         2-bromopropyl, 3-bromopropyl, 3,3,3-trifluoropropyl,         3,3,3-trichloropropyl, 2,2,3,3,3-pentafluoropropyl,         heptafluoropropyl, 1-(fluoromethyl)-2-fluoroethyl,         1-(chloromethyl)-2-chloroethyl, 1-(bromomethyl)-2-bromoethyl,         4-fluorobutyl, 4-chlorobutyl, 4-bromobutyl, nonafluorobutyl,         1,1,2,2,-tetrafluoroethyl and         1-trifluoromethyl-1,2,2,2-tetrafluoroethyl;     -   C₁-C₆-haloalkyl: C₁-C₄-haloalkyl as mentioned above, and also,         for example, 5-fluoropentyl, 5-chloropentyl, 5-bromopentyl,         5-iodopentyl, undecafluoropentyl, 6-fluorohexyl, 6-chlorohexyl,         6-bromohexyl, 6-iodohexyl and dodecafluorohexyl;     -   C₃-C₆-cycloalkyl: monocyclic saturated hydrocarbons having 3 to         6 ring members, such as cyclopropyl, cyclobutyl, cyclopentyl and         cyclohexyl;     -   C₂-C₆-alkenyl: for example ethenyl, 1-propenyl, 2-propenyl,         1-methylethenyl, 1-butenyl, 2-butenyl, 3-butenyl,         1-methyl-1-propenyl, 2-methyl-1-propenyl, 1-methyl-2-propenyl,         2-methyl-2-propenyl, 1-pentenyl, 2-pentenyl, 3-pentenyl,         4-pentenyl, 1-methyl-1-butenyl, 2-methyl-1-butenyl,         3-methyl-1-butenyl, 1-methyl-2-butenyl, 2-methyl-2-butenyl,         3-methyl-2-butenyl, 1-methyl-3-butenyl, 2-methyl-3-butenyl,         3-methyl-3-butenyl, 1,1-dimethyl-2-propenyl,         1,2-dimethyl-1-propenyl, 1,2-dimethyl-2-propenyl,         1-ethyl-1-propenyl, 1-ethyl-2-propenyl, 1-hexenyl, 2-hexenyl,         3-hexenyl, 4-hexenyl, 5-hexenyl, 1-methyl-1-pentenyl,         2-methyl-1-pentenyl, 3-methyl-1-pentenyl, 4-methyl-1-pentenyl,         1-methyl-2-pentenyl, 2-methyl-2-pentenyl, 3-methyl-2-pentenyl,         4-methyl-2-pentenyl, 1-methyl-3-pentenyl, 2-methyl-3-pentenyl,         3-methyl-3-pentenyl, 4-methyl-3-pentenyl, 1-methyl-4-pentenyl,         2-methyl-4-pentenyl, 3-methyl-4-pentenyl, 4-methyl-4-pentenyl,         1,1-dimethyl-2-butenyl, 1,1-dimethyl-3-butenyl,         1,2-dimethyl-1-butenyl, 1,2-dimethyl-2-butenyl,         1,2-dimethyl-3-butenyl, 1,3-dimethyl-1-butenyl,         1,3-dimethyl-2-butenyl, 1,3-dimethyl-3-butenyl,         2,2-dimethyl-3-butenyl, 2,3-dimethyl-1-butenyl,         2,3-dimethyl-2-butenyl, 2,3-dimethyl-3-butenyl,         3,3-dimethyl-1-butenyl, 3,3-dimethyl-2-butenyl,         1-ethyl-1-butenyl, 1-ethyl-2-butenyl, 1-ethyl-3-butenyl,         2-ethyl-1-butenyl, 2-ethyl-2-butenyl, 2-ethyl-3-butenyl,         1,1,2-trimethyl-2-propenyl, 1-ethyl-1-methyl-2-propenyl,         1-ethyl-2-methyl-1-propenyl and 1-ethyl-2-methyl-2-propenyl;     -   C₃-C₆-cycloalkenyl: 1-cyclopropenyl, 2-cyclopropenyl,         1-cyclobutenyl, 2-cyclobutenyl, 1-cyclopentenyl,         2-cyclopentenyl, 1,3-cyclopentadienyl, 1,4-cyclopentadienyl,         2,4-cyclopentadienyl, 1-cyclohexenyl, 2-cyclohexenyl,         3-cyclohexenyl, 1,3-cyclohexadienyl, 1,4-cyclohexadienyl,         2,5-cyclohexadienyl;     -   C₃-C₆-alkynyl: for example 1-propynyl, 2-propynyl, 1-butynyl,         2-butynyl, 3-butynyl, 1-methyl-2-propynyl, 1-pentynyl,         2-pentynyl, 3-pentynyl, 4-pentynyl, 1-methyl-2-butynyl,         1-methyl-3-butynyl, 2-methyl-3-butynyl, 3-methyl-1-butynyl,         1,1-dimethyl-2-propynyl, 1-ethyl-2-propynyl, 1-hexynyl,         2-hexynyl, 3-hexynyl, 4-hexynyl, 5-hexynyl, 1-methyl-2-pentynyl,         1-methyl-3-pentynyl, 1-methyl-4-pentynyl, 2-methyl-3-pentynyl,         2-methyl-4-pentynyl, 3-methyl-1-pentynyl, 3-methyl-4-pentynyl,         4-methyl-1-pentynyl, 4-methyl-2-pentynyl,         1,1-dimethyl-2-butynyl, 1,1-dimethyl-3-butynyl,         1,2-dimethyl-3-butynyl, 2,2-dimethyl-3-butynyl,         3,3-dimethyl-1-butynyl, 1-ethyl-2-butynyl, 1-ethyl-3-butynyl,         2-ethyl-3-butynyl and 1-ethyl-1-methyl-2-propynyl;     -   C₁-C₄-alkoxy: for example methoxy, ethoxy, propoxy,         1-methylethoxy butoxy, 1-methylpropoxy, 2-methylpropoxy and         1,1-dimethylethoxy;     -   C₁-C₆-alkoxy and also the C₁-C₆-alkoxy moieties of         (C₁-C₆-alkoxy)carbonyl, C₁-C₆-alkoxy-C₁-C₆-alkyl: C₁-C₄-alkoxy         as mentioned above, and also, for example, pentoxy,         1-methylbutoxy, 2-methylbutoxy, 3-methoxylbutoxy,         1,1-dimethylpropoxy, 1,2-dimethylpropoxy, 2,2-dimethylpropoxy,         1-ethylpropoxy, hexoxy, 1-methylpentoxy, 2-methylpentoxy,         3-methylpentoxy, 4-methylpentoxy, 1,1-dimethylbutoxy,         1,2-dimethylbutoxy, 1,3-dimethylbutoxy, 2,2-dimethylbutoxy,         2,3-dimethylbutoxy, 3,3-dimethylbutoxy, 1-ethylbutoxy,         2-ethylbutoxy, 1,1,2-trimethylpropoxy, 1,2,2-trimethylpropoxy,         1-ethyl-1-methylpropoxy and 1-ethyl-2-methylpropoxy.     -   C₁-C₄-alkylthio: for example methylthio, ethylthio, propylthio,         1-methylethylthio, butylthio, 1-methylpropylthio,         2-methylpropylthio and 1,1-dimethylethylthio;     -   C₁-C₆-alkylthio: C₁-C₄-alkylthio as mentioned above, and also,         for example, pentylthio, 1-methylbutylthio, 2-methylbutylthio,         3-methylbutylthio, 2,2-dimethylpropylthio, 1-ethylpropylthio,         hexylthio, 1,1-dimethylpropylthio, 1,2-dimethylpropylthio,         1-methylpentylthio, 2-methylpentylthio, 3-methylpentylthio,         4-methylpentylthio, 1,1-dimethylbutylthio,         1,2-dimethylbutylthio, 1,3-dimethylbutylthio,         2,2-dimethylbutylthio, 2,3-dimethylbutylthio,         3,3-dimethylbutylthio, 1-ethylbutylthio, 2-ethylbutylthio,         1,1,2-trimethylpropylthio, 1,2,2-trimethylpropylthio,         1-ethyl-1-methylpropylthio and 1-ethyl-2-methylpropylthio;     -   C₁-C₆-alkylsulfinyl (C₁-C₆-alkyl-S(═O)—): z.B. methylsulfinyl,         ethylsulfinyl, propylsulfinyl, 1-methylethylsulfinyl,         butylsulfinyl, 1-methylpropylsulfinyl, 2-methylpropylsulfinyl,         1,1-di-methylethylsulfinyl, pentylsulfinyl,         1-methylbutylsulfinyl, 2-methylbutylsulfinyl,         3-methylbutylsulfinyl, 2,2-dimethylpropylsulfinyl,         1-ethylpropylsulfinyl, 1,1-dimethylpropylsulfinyl,         1,2-dimethylpropylsulfinyl, hexylsulfinyl,         1-methylpentylsulfinyl, 2-methylpentylsulfinyl, 3-methyl         pentylsulfinyl, 4-methylpentyl-sulfinyl,         1,1-dimethylbutylsulfinyl, 1,2-dimethylbutylsulfinyl,         1,3-dimethylbutyl-sulfinyl, 2,2-dimethylbutylsulfinyl,         2,3-dimethylbutylsulfinyl, 3,3-dimethylbutyl-sulfinyl,         1-ethylbutylsulfinyl, 2-ethylbutylsulfinyl,         1,1,2-trimethylpropylsulfinyl, 1,2,2-trimethylpropylsulfinyl,         1-ethyl-1-methylpropylsulfinyl and 1-ethyl-2-methyl         propylsulfinyl;     -   C₁-C₆-alkylsulfonyl (C₁-C₆-alkyl-S(O)₂—): for example         methylsulfonyl, ethylsulfonyl, propylsulfonyl,         1-methylethylsulfonyl, butylsulfonyl, 1-methylpropylsulfonyl,         2-methylpropylsulfonyl, 1,1-dimethylethylsulfonyl,         pentylsulfonyl, 1-methylbutylsulfonyl, 2-methylbutylsulfonyl,         3-methylbutylsulfonyl, 1,1-dimethylpropylsulfonyl,         1,2-dimethylpropylsulfonyl, 2,2-dimethylpropylsulfonyl,         1-ethylpropylsulfonyl, hexylsulfonyl, 1-methylpentylsulfonyl,         2-methylpentylsulfonyl, 3-methylpentylsulfonyl,         4-methylpentylsulfonyl, 1,1-dimethylbutylsulfonyl,         1,2-dimethylbutylsulfonyl, 1,3-dimethylbutylsulfonyl,         2,2-dimethylbutylsulfonyl, 2,3-dimethylbutylsulfonyl,         3,3-dimethylbutylsulfonyl, 1-ethylbutylsulfonyl,         2-ethylbutylsulfonyl, 1,1,2-trimethyl-propylsulfonyl,         1,2,2-trimethylpropylsulfonyl, 1-ethyl-1-methylpropylsulfonyl         and 1-ethyl-2-methylpropylsulfonyl;     -   (C₁-C₄-alkyl)amino: for example methylamino, ethylamino,         propylamino, 1-methylethyl-amino, butylamino,         1-methylpropylamino, 2-methylpropylamino or         1,1-dimethylethylamino;     -   (C₁-C₆-alkyl)amino: (C₁-C₄-alkylamino) as mentioned above, and         also, for example, pentylamino, 1-methylbutylamino,         2-methylbutylamino, 3-methylbutylamino, 2,2-dimethylpropylamino,         1-ethylpropylamino, hexylamino, 1,1-dimethylpropylamino,         1,2-dimethylpropylamino, 1-methylpentylamino,         2-methylpentylamino, 3-methylpentylamino, 4-methylpentylamino,         1,1-dimethylbutylamino, 1,2-dimethylbutylamino,         1,3-dimethylbutylamino, 2,2-dimethylbutylamino,         2,3-dimethylbutyl-amino 3,3-dimethylbutyl-amino,         1-ethylbutylamino, 2-ethylbutylamino,         1,1,2-trimethylpropylamino, 1,2,2-trimethyl-propylamino,         1-ethyl-1-methylpropylamino or 1-ethyl-2-methylpropylamino;     -   di(C₁-C₄-alkyl)amino: for example N,N-dimethylamino,         N,N-diethylamino, N,N-di(1-methylethyl)amino, N,N-dipropylamino,         N,N-dibutylamino, N,N-di(1-methylpropyl)amino,         N,N-di(2-methylpropyl)amino, N,N-di(1,1-dimethylethyl)amino,         N-ethyl-N-methylamino, N-methyl-N-propylamino,         N-methyl-N-(1-methylethyl)amino, N-butyl-N-methylamino,         N-methyl-N-(1-methylpropyl)amino,         N-methyl-N-(2-methylpropyl)amino,         N-(1,1-dimethylethyl)-N-methylamino, N-ethyl-N-propylamino,         N-ethyl-N-(1-methylethyl)amino, N-butyl-N-ethylamino,         N-ethyl-N-(1-methylpropyl)amino,         N-ethyl-N-(2-methylpropyl)amino,         N-ethyl-N-(1,1-dimethylethyl)amino,         N-(1-methylethyl)-N-propylamino, N-butyl-N-propylamino,         N-(1-methylpropyl)-N-propylamino,         N-(2-methylpropyl)-N-propylamino,         N-(1,1-dimethylethyl)-N-propylamino,         N-butyl-N-(1-methylethyl)amino,         N-(1-methylethyl)-N-(1-methylpropyl)amino,         N-(1-methylethyl)-N-(2-methylpropyl)amino,         N-(1,1-dimethylethyl)-N-(1-methylethyl)amino,         N-butyl-N-(1-methylpropyl)amino,         N-butyl-N-(2-methylpropyl)amino,         N-butyl-N-(1,1-dimethylethyl)amino,         N-(1-methylpropyl)-N-(2-methylpropyl)amino,         N-(1,1-dimethylethyl)-N-(1-methylpropyl)amino or         N-(1,1-dimethylethyl)-N-(2-methylpropyl)amino;     -   di(C₁-C₆-alkyl)amino: di(C₁-C₄-alkyl)amino as mentioned above,         and also, for example, N-methyl-N-pentylamino,         N-methyl-N-(1-methylbutyl)amino,         N-methyl-N-(2-methyl-butyl)amino,         N-methyl-N-(3-methylbutyl)amino,         N-methyl-N-(2,2-dimethylpropyl)amino,         N-methyl-N-(1-ethylpropyl)amino, N-methyl-N-hexylamino,         N-methyl-N-(1,1-dimethyl-propyl)amino,         N-methyl-N-(1,2-dimethylpropyl)amino,         N-methyl-N-(1-methylpentyl)amino,         N-methyl-N-(2-methylpentyl)amino,         N-methyl-N-(3-methylpentyl)amino,         N-methyl-N-(4-methylpentyl)amino,         N-methyl-N-(1,1-dimethylbutyl)amino,         N-methyl-N-(1,2-dimethylbutyl)amino,         N-methyl-N-(1,3-dimethylbutyl)amino,         N-methyl-N-(2,2-dimethyl-butyl)amino,         N-methyl-N-(2,3-dimethylbutyl)amino,         N-methyl-N-(3,3-dimethylbutyl)amino,         N-methyl-N-(1-ethylbutyl)amino, N-methyl-N-(2-ethylbutyl)amino,         N-methyl-N-(1,1,2-trimethylpropyl)amino,         N-methyl-N-(1,2,2-trimethylpropyl)amino,         N-methyl-N-(1-ethyl-1-methylpropyl)amino,         N-methyl-N-(1-ethyl-2-methylpropyl)amino, N-ethyl-N-pentylamino,         N-ethyl-N-(1-methylbutyl)amino, N-ethyl-N-(2-methylbutyl)amino,         N-ethyl-N-(3-methylbutyl)amino,         N-ethyl-N-(2,2-dimethylpropyl)amino,         N-ethyl-N-(1-ethylpropyl)amino, N-ethyl-N-hexylamino,         N-ethyl-N-(1,1-dimethylpropyl)amino,         N-ethyl-N-(1,2-dimethylpropyl)amino,         N-ethyl-N-(1-methylpentyl)amino,         N-ethyl-N-(2-methylpentyl)amino,         N-ethyl-N-(3-methylpentyl)amino,         N-ethyl-N-(4-methylpentyl)amino,         N-ethyl-N-(1,1-dimethylbutyl)amino,         N-ethyl-N-(1,2-dimethylbutyl)amino,         N-ethyl-N-(1,3-dimethylbutyl)amino,         N-ethyl-N-(2,2-dimethylbutyl)amino,         N-ethyl-N-(2,3-dimethylbutyl)amino,         N-ethyl-N-(3,3-dimethylbutyl)amino,         N-ethyl-N-(1-ethylbutyl)amino, N-ethyl-N-(2-ethylbutyl)amino,         N-ethyl-N-(1,1,2-trimethylpropyl)amino,         N-ethyl-N-(1,2,2-trimethylpropyl)amino,         N-ethyl-N-(1-ethyl-1-methylpropyl)amino,         N-ethyl-N-(1-ethyl-2-methylpropyl)amino, N-propyl-N-pentylamino,         N-butyl-N-pentylamino, N,N-dipentylamino, N-propyl-N-hexylamino,         N-butyl-N-hexylamino, N-pentyl-N-hexylamino or N,N-dihexylamino;     -   three- to six-membered heterocyclyl: monocyclic saturated or         partially unsaturated hydrocarbon having three to six ring         members as mentioned above which, in addition to carbon atoms,         contains one or two heteroatoms selected from O, S and N;         for example 2-oxiranyl, 2-oxetanyl, 3-oxetanyl, 2-aziridinyl,         3-thietanyl, 1-azetidinyl, 2-azetidinyl,         for example 2-tetrahydrofuranyl, 3-tetrahydrofuranyl,         2-tetrahydrothienyl, 3-tetrahydrothienyl, 2-pyrrolidinyl,         3-pyrrolidinyl, 3-isoxazolidinyl, 4-isoxazolidinyl,         5-isoxazolidinyl, 3-isothiazolidinyl, 4-isothiazolidinyl,         5-isothiazolidinyl, 3-pyrazolidinyl, 4-pyrazolidinyl,         5-pyrazolidinyl, 2-oxazolidinyl, 4-oxazolidinyl, 5-oxazolidinyl,         2-thiazolidinyl, 4-thiazolidinyl, 5-thiazolidinyl,         2-imidazolidinyl, 4-imidazolidinyl;         for example 2,3-dihydrofur-2-yl, 2,3-dihydrofur-3-yl,         2,4-dihydrofur-2-yl, 2,4-dihydrofur-3-yl, 2,3-dihydrothien-2-yl,         2,3-dihydrothien-3-yl, 2,4-dihydrothien-2-yl,         2,4-dihydrothien-3-yl, 4,5-dihydropyrrol-2-yl,         4,5-dihydropyrrol-3-yl, 2,5-dihydropyrrol-2-yl,         2,5-dihydropyrrol-3-yl, 4,5-dihydroisoxazol-3-yl,         2,5-dihydroisoxazol-3-yl, 2,3-dihydroisoxazol-3-yl,         4,5-dihydroisoxazol-4-yl, 2,5-dihydroisoxazol-4-yl,         2,3-dihydroisoxazol-4-yl, 4,5-dihydroisoxazol-5-yl,         2,5-dihydroisoxazol-5-yl, 2,3-dihydroisoxazol-5-yl,         4,5-dihydroisothiazol-3-yl, 2,5-dihydroisothiazol-3-yl,         2,3-dihydroisothiazol-3-yl, 4,5-dihydroisothiazol-4-yl,         2,5-dihydroisothiazol-4-yl, 2,3-dihydroisothiazol-4-yl,         4,5-dihydroisothiazol-5-yl, 2,5-dihydroisothiazol-5-yl,         2,3-dihydroisothiazol-5-yl, 2,3-dihydropyrazol-2-yl,         2,3-dihydropyrazol-3-yl, 2,3-dihydropyrazol-4-yl,         2,3-dihydropyrazol-5-yl, 3,4-dihydropyrazol-3-yl,         3,4-dihydropyrazol-4-yl, 3,4-dihydropyrazol-5-yl,         4,5-dihydropyrazol-3-yl, 4,5-dihydropyrazol-4-yl,         4,5-dihydropyrazol-5-yl, 2,3-dihydroimidazol-2-yl,         2,3-dihydroimidazol-3-yl, 2,3-dihydroimidazol-4-yl,         2,3-dihydroimidazol-5-yl, 4,5-dihydroimidazol-2-yl,         4,5-di-hydroimidazol-4-yl, 4,5-dihydroimidazol-5-yl,         2,5-dihydroimidazol-2-yl, 2,5-dihydroimidazol-4-yl,         2,5-dihydroimidazol-5-yl, 2,3-dihydrooxazol-3-yl,         2,3-dihydrooxazol-4-yl, 2,3-dihydrooxazol-5-yl,         3,4-dihydrooxazol-3-yl, 3,4-dihydrooxazol-4-yl,         3,4-dihydrooxazol-5-yl, 2,3-dihydrothiazol-3-yl,         2,3-dihydrothiazol-4-yl, 2,3-dihydrothiazol-5-yl,         3,4-dihydrothiazol-3-yl, 3,4-dihydrothiazol-4-yl,         3,4-dihydrothiazol-5-yl, 3,4-dihydrothiazol-2-yl,         3,4-dihydrothiazol-3-yl, 3,4-dihydrothiazol-4-yl;         for example 2-piperidinyl, 3-piperidinyl, 4-piperidinyl,         1,3-dioxan-2-yl, 1,3-dioxan-4-yl, 1,3-dioxan-5-yl,         1,4-dioxan-2-yl, 1,3-dithian-2-yl, 1,3-dithian-4-yl,         1,4-dithian-2-yl, 1,3-dithian-5-yl, 2-tetrahydropyranyl,         3-tetrahydropyranyl, 4-tetrahydropyranyl,         2-tetrahydrothiopyranyl, 3-tetrahydrothiopyranyl,         4-tetrahydro-thiopyranyl, 3-hexahydropyridazinyl,         4-hexahydropyridazinyl, 2-hexahydropyrimidinyl,         4-hexahydropyrimidinyl, 5-hexahydropyrimidinyl, 2-piperazinyl,         tetrahydro-1,3-oxazin-2-yl, tetrahydro-1,3-oxazin-6-yl,         2-morpholinyl, 3-morpholinyl;         for example 2H-pyran-2-yl, 2H-pyran-3-yl, 2H-pyran-4-yl,         2H-pyran-5-yl, 2H-pyran-6-yl, 3,6-dihydro-2H-pyran-2-yl,         3,6-dihydro-2H-pyran-3-yl, 3,6-dihydro-2H-pyran-4-yl,         3,6-dihydro-2H-pyran-5-yl, 3,6-dihydro-2H-pyran-6-yl,         3,4-dihydro-2H-pyran-3-yl, 3,4-dihydro-2H-pyran-4-yl,         3,4-dihydro-2H-pyran-6-yl, 2H-thiopyran-2-yl, 2H-thiopyran-3-yl,         2H-thiopyran-4-yl, 2H-thiopyran-5-yl, 2H-thiopyran-6-yl,         5,6-dihydro-4H-1,3-oxazin-2-yl;

The preferred embodiments of the invention mentioned herein below have to be understood as being preferred either independently from each other or in combination with one another.

According to a preferred embodiment of the invention preference is also given to those azines of formula (I), wherein the variables, either independently of one another or in combination with one another, have the following meanings:

Preferred are the azines of formula (I), wherein

-   A is phenyl, which is substituted by two to five substituents     selected from the group consisting of halogen, CN, NO₂, C₁-C₆-alkyl,     C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy, C₁-C₆-alkylthio,     (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl, amino,     (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)carbonyl,     (C₁-C₆-alkoxy)carbonyl;     -   particularly preferred phenyl, which is substituted by two to         five substituents selected from the group consisting of halogen,         CN, C₁-C₆-alkyl and C₁-C₆-alkoxy; particularly preferred         selected from halogen and CN;         -   also particularly preferred selected from the group             consisting of F, Cl, CN and CH₃;         -   especially preferred selected from the group consisting of             F, Cl and CN;     -   especially preferred phenyl, which is substituted by two to four         substituents selected from the group consisting of halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   more preferred phenyl, which is substituted by two substituents         -   selected from the group consisting of halogen, CN, NO₂,             C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,             C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,             (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,             di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,             (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   also more preferred phenyl, which is substituted by three         substituents selected from the group consisting of halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   also more preferred phenyl, which is substituted by four         substituents         -   selected from the group consisting of halogen, CN, NO₂,             C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,             C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,             (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,             di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,             (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN.

Also preferred are the azines of formula (I), wherein

A is

-   -   wherein     -   R^(a) and R^(e) independently of one another are halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl; and     -   R^(b), R^(c) and R^(d) independently of one another are         hydrogen, halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH,         C₁-C₆-alkoxy, C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,         (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,         di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,         (C₁-C₆-alkoxy)carbonyl;     -   particularly preferred R^(a) and R^(e) independently of one         another are halogen, CN, C₁-C₆-alkyl or C₁-C₆-alkoxy; and         -   R^(b), R^(c) and R^(d) independently of one another are             hydrogen, halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl or             C₁-C₆-alkoxy;     -   especially preferred R^(a) and R^(e) independently of one         another are halogen or CN; and         -   R^(b), R^(c) and R^(d) independently of one another are             hydrogen, halogen, CN, C₁-C₆-alkyl or C₁-C₆-alkoxy;     -   more preferred R^(a) and R^(e) are halogen; and         -   R^(b), R^(c) and R^(d) independently of one another are             hydrogen, halogen or CN;     -   most preferred R^(a) and R^(e) are halogen; and         -   R^(b), R^(c) and R^(d) are hydrogen;     -   also most preferred R^(a), R^(b), R^(d) and R^(e) are halogen;         and         -   R^(c) hydrogen;     -   also most preferred R^(a), R^(b), R^(c), R^(d) and R^(e) are         halogen.

Also preferred are the azines of formula (I), wherein

A is

-   -   wherein R^(a) is halogen or CN;         -   R^(b) and R^(d) are H, halogen or CN;         -   R^(c) is H or halogen;         -   R^(e) is halogen, CN or C₁-C₆-alkyl;     -   particularly preferred R^(a) is halogen;         -   R^(b), R^(c) and R^(d) are H or halogen; and         -   R^(e) is halogen or CN;     -   especially preferred R^(a), R^(b), R^(d) and R^(e) are halogen;         and         -   R^(c) is H or halogen;     -   more preferred R^(a), R^(b), R^(d) and R^(e) are F; and         -   R^(c) is H or F.

Especially preferred are the azines of formula (I), wherein A is selected from the group consisting of (A.1.1), (A.1.2) and (A.1.3);

more preferred selected from the group consisting of (A.1.2) and (A.1.3);

-   -   wherein     -   R^(a) and R^(e) independently of one another are halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl; and     -   R^(b) and R^(d) independently of one another are halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl;     -   particularly preferred R^(a) and R^(e) independently of one         another are halogen, CN, C₁-C₆-alkyl or C₁-C₆-alkoxy; and         -   R^(b) and R^(d) independently of one another are halogen,             CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl or C₁-C₆-alkoxy;     -   especially preferred R^(a) and R^(e) independently of one         another halogen or CN; and         -   R^(b) and R^(d) independently of one another are halogen,             CN, C₁-C₆-alkyl or C₁-C₆-alkoxy;     -   more preferred R^(a) and R^(e) are halogen; and         -   R^(b) and R^(d) independently of one another are halogen or             CN;     -   most preferred R^(a), R^(b), R^(d) and R^(e) are halogen.

Also especially preferred are the azines of formula (I), wherein

A is

-   -   wherein R^(a), R^(b), R^(d) and R^(e) have the meanings, in         particular the preferred meanings, as defined above.

Also especially preferred are the azines of formula (I), wherein

A is

-   -   wherein R^(a), R^(b) and R^(e) have the meanings, in particular         the preferred meanings, as defined above.

Also especially preferred are the azines of formula (I), wherein

A is

-   -   wherein R^(a) and R^(e) have the meanings, in particular the         preferred meanings, as defined above.

Also preferred are the azines of formula (I), wherein

-   A is 2-fluoro-phenyl, which is substituted by one to four     substituents selected from the group consisting of halogen, CN, NO₂,     C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy, C₁-C₆-alkylthio,     (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl, amino,     (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)carbonyl and     (C₁-C₆-alkoxy)carbonyl;     -   particularly preferred 2-fluoro-phenyl, which is substituted by         one to four substituents selected from the group consisting of         halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy; particularly         preferred selected from halogen and CN;         -   also particularly preferred selected from the group             consisting of F, Cl, CN and CH₃;         -   especially preferred selected from the group consisting of             F, Cl and CN;     -   especially preferred 2-fluoro-phenyl, which is substituted by         one to three substituents selected from the group consisting of         halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH,         C₁-C₆-alkoxy, C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,         (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,         di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl and         (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   more preferred 2-fluoro-phenyl, which is substituted by one         substituent selected from the group consisting of halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl and (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   also more preferred 2-fluoro-phenyl, which is substituted by two         substituents selected from the group consisting of halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl and (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   also more preferred 2-fluoro-phenyl, which is substituted by         three substituents selected from the group consisting of         halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH,         C₁-C₆-alkoxy, C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,         (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,         di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl and         (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN.

Also preferred are the azines of formula (I), wherein

A is

-   -   wherein     -   R^(a) is halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH,         C₁-C₆-alkoxy, C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,         (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,         di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)carbonyl,         (C₁-C₆-alkoxy)carbonyl; and     -   R^(b), R^(c) and R^(d) independently of one another are         hydrogen, halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH,         C₁-C₆-alkoxy, C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,         (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,         di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,         (C₁-C₆-alkoxy)carbonyl;     -   particularly preferred R^(a) is halogen, CN, C₁-C₆-alkyl or         C₁-C₆-alkoxy; and         -   R^(b), R^(c) and R^(d) independently of one another are             hydrogen, halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl or             C₁-C₆-alkoxy;     -   especially preferred R^(a) is halogen or CN; and         -   R^(b), R^(c) and R^(d) independently of one another are             hydrogen, halogen, CN, C₁-C₆-alkyl or C₁-C₆-alkoxy;     -   more preferred R^(a) is halogen; and         -   R^(b), R^(c) and R^(d) independently of one another are             hydrogen, halogen or CN;     -   most preferred R^(a) is halogen; and         -   R^(b), R^(c) and R^(d) are hydrogen;     -   also most preferred R^(a), R^(b) and R^(d) are halogen; and         -   R^(c) is hydrogen;     -   also most preferred R^(a), R^(b), R^(c) and R^(d) are halogen.

Also preferred are the azines of formula (I), wherein

A is

-   -   wherein R^(a) is halogen, CN or C₁-C₆-alkyl;         -   R^(b) and R^(d) are H, halogen or CN; and         -   R^(c) is H or halogen;     -   particularly preferred R^(a) is halogen or CN; and         -   R^(b), R^(c) and R^(d) are H or halogen;     -   especially preferred R^(a), R^(b) and R^(d) are halogen; and         -   R^(c) is H or halogen;     -   Also especially preferred R^(a), Rb and R^(d) are halogen; and         -   R^(c) is H, F, Br or I;     -   more preferred R^(a), Rb and R^(d) are F; and         -   R^(c) is F, Br or I;     -   also more preferred R^(a), Rb and R^(d) are F; and         -   R^(c) is H or F.

Especially preferred are the azines of formula (I), wherein A is selected from the group consisting of (A.1a.1), (A.1a.2) and (A.1a.3);

more preferred selected from the group consisting of (A.1.2) and (A.1.3);

-   -   wherein     -   R^(a) is halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH,         C₁-C₆-alkoxy, C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,         (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,         di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)carbonyl,         (C₁-C₆-alkoxy)carbonyl; and     -   R^(b) and R^(d) independently of one another are halogen, CN,         NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         amino, (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl;     -   particularly preferred R^(a) is halogen, CN, C₁-C₆-alkyl or         C₁-C₆-alkoxy; and         -   R^(b) and R^(d) independently of one another are halogen,             CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl or C₁-C₆-alkoxy;     -   especially preferred R^(a) is halogen or CN; and         -   R^(b) and R^(d) independently of one another are halogen,             CN, C₁-C₆-alkyl or C₁-C₆-alkoxy;     -   more preferred R^(a) is halogen; and         -   R^(b) and R^(d) independently of one another are halogen or             CN;     -   most preferred R^(a), R^(b) and R^(d) are halogen.

Also especially preferred are the azines of formula (I), wherein

A is

-   -   wherein R^(a), R^(b) and R^(d) have the meanings, in particular         the preferred meanings, as defined above.

Also especially preferred are the azines of formula (I), wherein

A is

-   -   wherein R^(a) and R^(b) have the meanings, in particular the         preferred meanings, as defined above.

Also especially preferred are the azines of formula (I), wherein

A is

-   -   wherein R^(a) has the meanings, in particular the preferred         meanings, as defined above.

Also preferred are the azines of formula (I), wherein

-   R¹ is H, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy-C₁-C₆-alkyl,     C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl or (C₁-C₆-alkyl)sulfonyl;     -   particularly preferred H, CN, C₁-C₆-alkyl,         C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl or         (C₁-C₆-alkyl)sulfonyl;     -   especially preferred H, CN, CH₃, CH₂OCH₃, OCH₃, COCH₃ or SO₂CH₃;     -   more preferred hydrogen.

Also preferred are the azines of formula (I), wherein

-   R² is H, halogen, C₁-C₆-alkyl or C₁-C₆-haloalkyl;     -   particularly preferred halogen, C₁-C₆-alkyl or C₁-C₆-haloalkyl;     -   also particularly preferred H, F, Cl, CH₃ or CF₃.

Also preferred are the azines of formula (I), wherein

-   R³ and R⁴ are     -   independently of one another H, halogen, C₁-C₆-alkyl or         C₁-C₆-haloalkyl; or together with the carbon atom to which they         are attached form a moiety selected from the group consisting of         C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl and three- to six-membered         heterocyclyl,         -   wherein the C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl or the             three- to six-membered heterocyclyl is unsubstituted or             substituted by one to three substituents selected from             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;     -   independently of one another particularly preferred H, halogen,         C₁-C₆-alkyl or C₁-C₆-haloalkyl; or     -   together with the carbon atom to which they are attached form a         moiety selected from the group consisting of C₃-C₆-cycloalkyl         and C₃-C₆-cycloalkenyl,         -   wherein the C₃-C₆-cycloalkyl or C₃-C₆-cycloalkenyl is             unsubstituted or substituted by one to three substituents             selected from halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;     -   independently of one another especially preferred H, halogen,         C₁-C₆-alkyl or C₁-C₆-haloalkyl;     -   independently of one another more preferred H, halogen or         C₁-C₆-alkyl.

Also preferred are the azines of formula (I), wherein

-   -   R² is H, halogen, C₁-C₆-alkyl; and     -   R³ and R⁴ are independently of one another H, halogen,         C₁-C₆-alkyl, or together with the carbon atom to which they are         attached form a C₃-C₆-cycloalkyl;     -   particularly preferred R² is H, halogen or C₁-C₆-alkyl;         -   R³ is C₁-C₆-alkyl;         -   R⁴ is H, halogen or C₁-C₆-alkyl;         -   R³ and R⁴ together with the carbon atom to which they are             attached form a C₃-C₆-cycloalkyl;     -   especially preferred R² is halogen or C₁-C₆-alkyl;         -   R³ is C₁-C₆-alkyl;         -   R⁴ is H or C₁-C₆-alkyl;     -   more preferred R² is halogen; and         -   R³ and R⁴ are C₁-C₆-alkyl.

Also preferred are the azines of formula (I), wherein

-   R⁵ is H, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy-C₁-C₆-alkyl,     C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl or (C₁-C₆-alkyl)sulfonyl;     -   particularly preferred H, CN, C₁-C₆-alkyl,         C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl or         (C₁-C₆-alkyl)sulfonyl;     -   especially preferred H, CN, CH₃, CH₂OCH₃, OCH₃, COCH₃ or SO₂CH₃;     -   more preferred hydrogen.

Also preferred are the azines of formula (I), wherein

-   -   A is phenyl, which is substituted by two to five substituents         -   selected from the group consisting of halogen, CN,             C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   particularly preferred selected from halogen and CN;         -   also particularly preferred selected from the group             consisting of F, Cl, CN and CH₃;         -   especially preferred selected from the group consisting of             F, Cl and CN;     -   particularly preferred phenyl, which is substituted by two to         four substituents         -   selected from the group consisting of halogen, CN, NO₂,             C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,             C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,             (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,             di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,             (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   especially preferred phenyl, which is substituted by two         substituents         -   selected from the group consisting of halogen, CN, NO₂,             C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,             C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,             (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,             di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,             (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   also especially preferred phenyl, which is substituted by three         substituents         -   selected from the group consisting of halogen, CN, NO₂,             C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,             C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,             (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,             di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,             (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   also specially preferred phenyl, which is substituted by four         substituents         -   selected from the group consisting of halogen, CN, NO₂,             C₁-C₆-alkyl, C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy,             C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl,             (C₁-C₆-alkyl)sulfonyl, amino, (C₁-C₆-alkyl)amino,             di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,             (C₁-C₆-alkoxy)carbonyl;         -   particularly preferred selected from the group consisting of             halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   especially preferred selected from halogen and CN;         -   also especially preferred selected from the group consisting             of F, Cl, CN and CH₃;         -   more preferred selected from the group consisting of F, Cl             and CN;     -   R¹ is H, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl,         C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl or         (C₁-C₆-alkyl)sulfonyl;         -   particularly preferred H, CN, C₁-C₆-alkyl,             C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy,             (C₁-C₆-alkyl)carbonyl or (C₁-C₆-alkyl)sulfonyl;         -   especially preferred H, CN, CH₃, CH₂OCH₃, OCH₃, COCH₃ or             SO₂CH₃;         -   more preferred hydrogen.     -   R² is H, halogen, C₁-C₆-alkyl or C₁-C₆-haloalkyl;         -   particularly preferred halogen, C₁-C₆-alkyl or             C₁-C₆-haloalkyl;         -   also particularly preferred H, F, CH₃ or CF₃;     -   R³ and R⁴ are independently of one another H, halogen,         C₁-C₆-alkyl or C₁-C₆-haloalkyl; or         -   together with the carbon atom to which they are attached             form a moiety selected from the group consisting of             C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl and three- to             six-membered heterocyclyl,             -   wherein the C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl or the                 three- to six-membered heterocyclyl is unsubstituted or                 substituted by one to three substituents selected from                 halogen, CN, C₁-C₆-alkyl and C₁-C₆-alkoxy;         -   independently of one another particularly preferred H,             halogen, C₁-C₆-alkyl or C₁-C₆-haloalkyl; or         -   together with the carbon atom to which they are attached             form a moiety selected from the group consisting of             C₃-C₆-cycloalkyl and C₃-C₆-cycloalkenyl,             -   wherein the C₃-C₆-cycloalkyl or C₃-C₆-cycloalkenyl is                 unsubstituted or substituted by one to three                 substituents selected from halogen, CN, C₁-C₆-alkyl and                 C₁-C₆-alkoxy;         -   independently of one another especially preferred H,             halogen, C₁-C₆-alkyl or C₁-C₆-haloalkyl;         -   independently of one another more preferred H, halogen or             C₁-C₆-alkyl; and     -   R⁵ is H, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl,         C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy, (C₁-C₆-alkyl)carbonyl or         (C₁-C₆-alkyl)sulfonyl;         -   particularly preferred H, CN, C₁-C₆-alkyl,             C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy,             (C₁-C₆-alkyl)carbonyl or (C₁-C₆-alkyl)sulfonyl;         -   especially preferred H, CN, CH₃, CH₂OCH₃, OCH₃, COCH₃ or             SO₂CH₃;         -   more preferred hydrogen.

Particular preference is given to azines of formula (I.a), which correspond to azines of formula (I) wherein A is (A.1) and R¹ and R⁵ are H:

-   -   wherein the variables R^(a), R^(b), R^(c), R^(d), R^(e), R², R³         and R⁴ have the meanings, in particular the preferred meanings,         as defined above;         special preference is given to the azines of the formulae         (I.a.1) to (I.a.1406) of Table A, where the definitions of the         variables R^(a), R^(b), R^(c), R^(d), R^(e), R², R³ and R⁴ are         of particular importance for the compounds according to the         invention not only in combination with one another but in each         case also on their own:

TABLE A No. R^(a) R^(b) R^(c) R^(d) R^(e) R² R³ R⁴ I.a.1 F H H H F CH₃ H H I.a.2 Cl H H H F CH₃ H H I.a.3 Br H H H F CH₃ H H I.a.4 CN H H H F CH₃ H H I.a.5 CH₃ H H H F CH₃ H H I.a.6 F H H F F CH₃ H H I.a.7 Cl H H F F CH₃ H H I.a.8 F H H Cl F CH₃ H H I.a.9 Cl H H F F CH₃ H H I.a.10 CN H H F F CH₃ H H I.a.11 F H H CN F CH₃ H H I.a.12 CN H H F F CH₃ H H I.a.13 F H F H F CH₃ H H I.a.14 Cl H F H F CH₃ H H I.a.15 CN H F H F CH₃ H H I.a.16 F F F H F CH₃ H H I.a.17 Cl F F H F CH₃ H H I.a.18 F Cl F H F CH₃ H H I.a.19 Cl F F H F CH₃ H H I.a.20 CN F F H F CH₃ H H I.a.21 F CN F H F CH₃ H H I.a.22 CN F F H F CH₃ H H I.a.23 F F H F F CH₃ H H I.a.24 Cl F H F F CH₃ H H I.a.25 F Cl H F F CH₃ H H I.a.26 CN F H F F CH₃ H H I.a.27 F CN H F F CH₃ H H I.a.28 F F F F F CH₃ H H I.a.29 Cl F F F F CH₃ H H I.a.30 F Cl F F F CH₃ H H I.a.31 CN F F F F CH₃ H H I.a.32 F CN F F F CH₃ H H I.a.33 H F F F F CH₃ H H I.a.34 F F Br F F CH₃ H H I.a.35 F F C≡CH F F CH₃ H H I.a.36 CF₃ Cl H H F CH₃ H H I.a.37 F F I F F CH₃ H H I.a.38 F H H H F CH₃ CH₃ H I.a.39 Cl H H H F CH₃ CH₃ H I.a.40 Br H H H F CH₃ CH₃ H I.a.41 CN H H H F CH₃ CH₃ H I.a.42 CH₃ H H H F CH₃ CH₃ H I.a.43 F H H F F CH₃ CH₃ H I.a.44 Cl H H F F CH₃ CH₃ H I.a.45 F H H Cl F CH₃ CH₃ H I.a.46 Cl H H F F CH₃ CH₃ H I.a.47 CN H H F F CH₃ CH₃ H I.a.48 F H H CN F CH₃ CH₃ H I.a.49 CN H H F F CH₃ CH₃ H I.a.50 F H F H F CH₃ CH₃ H I.a.51 Cl H F H F CH₃ CH₃ H I.a.52 CN H F H F CH₃ CH₃ H I.a.53 F F F H F CH₃ CH₃ H I.a.54 Cl F F H F CH₃ CH₃ H I.a.55 F Cl F H F CH₃ CH₃ H I.a.56 Cl F F H F CH₃ CH₃ H I.a.57 CN F F H F CH₃ CH₃ H I.a.58 F CN F H F CH₃ CH₃ H I.a.59 CN F F H F CH₃ CH₃ H I.a.60 F F H F F CH₃ CH₃ H I.a.61 Cl F H F F CH₃ CH₃ H I.a.62 F Cl H F F CH₃ CH₃ H I.a.63 CN F H F F CH₃ CH₃ H I.a.64 F CN H F F CH₃ CH₃ H I.a.65 F F F F F CH₃ CH₃ H I.a.66 Cl F F F F CH₃ CH₃ H I.a.67 F Cl F F F CH₃ CH₃ H I.a.68 CN F F F F CH₃ CH₃ H I.a.69 F CN F F F CH₃ CH₃ H I.a.70 H F F F F CH₃ CH₃ H I.a.71 F F Br F F CH₃ CH₃ H I.a.72 F F C≡CH F F CH₃ CH₃ H I.a.73 CF₃ Cl H H F CH₃ CH₃ H I.a.74 F F I F F CH₃ CH₃ H I.a.75 F H H H F CH₃ CH₃ CH₃ I.a.76 Cl H H H F CH₃ CH₃ CH₃ I.a.77 Br H H H F CH₃ CH₃ CH₃ I.a.78 CN H H H F CH₃ CH₃ CH₃ I.a.79 CH₃ H H H F CH₃ CH₃ CH₃ I.a.80 F H H F F CH₃ CH₃ CH₃ I.a.81 Cl H H F F CH₃ CH₃ CH₃ I.a.82 F H H Cl F CH₃ CH₃ CH₃ I.a.83 Cl H H F F CH₃ CH₃ CH₃ I.a.84 CN H H F F CH₃ CH₃ CH₃ I.a.85 F H H CN F CH₃ CH₃ CH₃ I.a.86 CN H H F F CH₃ CH₃ CH₃ I.a.87 F H F H F CH₃ CH₃ CH₃ I.a.88 Cl H F H F CH₃ CH₃ CH₃ I.a.89 CN H F H F CH₃ CH₃ CH₃ I.a.90 F F F H F CH₃ CH₃ CH₃ I.a.91 Cl F F H F CH₃ CH₃ CH₃ I.a.92 F Cl F H F CH₃ CH₃ CH₃ I.a.93 Cl F F H F CH₃ CH₃ CH₃ I.a.94 CN F F H F CH₃ CH₃ CH₃ I.a.95 F CN F H F CH₃ CH₃ CH₃ I.a.96 CN F F H F CH₃ CH₃ CH₃ I.a.97 F F H F F CH₃ CH₃ CH₃ I.a.98 Cl F H F F CH₃ CH₃ CH₃ I.a.99 F Cl H F F CH₃ CH₃ CH₃ I.a.100 CN F H F F CH₃ CH₃ CH₃ I.a.101 F CN H F F CH₃ CH₃ CH₃ I.a.102 F F F F F CH₃ CH₃ CH₃ I.a.103 Cl F F F F CH₃ CH₃ CH₃ I.a.104 F Cl F F F CH₃ CH₃ CH₃ I.a.105 CN F F F F CH₃ CH₃ CH₃ I.a.106 F CN F F F CH₃ CH₃ CH₃ I.a.107 H F F F F CH₃ CH₃ CH₃ I.a.108 F F Br F F CH₃ CH₃ CH₃ I.a.109 F F C≡CH F F CH₃ CH₃ CH₃ I.a.110 CF₃ Cl H H F CH₃ CH₃ CH₃ I.a.111 F F I F F CH₃ CH₃ CH₃ I.a.112 F H H H F F F F I.a.113 Cl H H H F F F F I.a.114 Br H H H F F F F I.a.115 CN H H H F F F F I.a.116 CH₃ H H H F F F F I.a.117 F H H F F F F F I.a.118 Cl H H F F F F F I.a.119 F H H Cl F F F F I.a.120 Cl H H F F F F F I.a.121 CN H H F F F F F I.a.122 F H H CN F F F F I.a.123 CN H H F F F F F I.a.124 F H F H F F F F I.a.125 Cl H F H F F F F I.a.126 CN H F H F F F F I.a.127 F F F H F F F F I.a.128 Cl F F H F F F F I.a.129 F Cl F H F F F F I.a.130 Cl F F H F F F F I.a.131 CN F F H F F F F I.a.132 F CN F H F F F F I.a.133 CN F F H F F F F I.a.134 F F H F F F F F I.a.135 Cl F H F F F F F I.a.136 F Cl H F F F F F I.a.137 CN F H F F F F F I.a.138 F CN H F F F F F I.a.139 F F F F F F F F I.a.140 Cl F F F F F F F I.a.141 F Cl F F F F F F I.a.142 CN F F F F F F F I.a.143 F CN F F F F F F I.a.144 H F F F F F F F I.a.145 F F Br F F F F F I.a.146 F F C≡CH F F F F F I.a.147 CF₃ Cl H H F F F F I.a.148 F F I F F F F F I.a.149 F H H H F F CF₃ F I.a.150 Cl H H H F F CF₃ F I.a.151 Br H H H F F CF₃ F I.a.152 CN H H H F F CF₃ F I.a.153 CH₃ H H H F F CF₃ F I.a.154 F H H F F F CF₃ F I.a.155 Cl H H F F F CF₃ F I.a.156 F H H Cl F F CF₃ F I.a.157 Cl H H F F F CF₃ F I.a.158 CN H H F F F CF₃ F I.a.159 F H H CN F F CF₃ F I.a.160 CN H H F F F CF₃ F I.a.161 F H F H F F CF₃ F I.a.162 Cl H F H F F CF₃ F I.a.163 CN H F H F F CF₃ F I.a.164 F F F H F F CF₃ F I.a.165 Cl F F H F F CF₃ F I.a.166 F Cl F H F F CF₃ F I.a.167 Cl F F H F F CF₃ F I.a.168 CN F F H F F CF₃ F I.a.169 F CN F H F F CF₃ F I.a.170 CN F F H F F CF₃ F I.a.171 F F H F F F CF₃ F I.a.172 Cl F H F F F CF₃ F I.a.173 F Cl H F F F CF₃ F I.a.174 CN F H F F F CF₃ F I.a.175 F CN H F F F CF₃ F I.a.176 F F F F F F CF₃ F I.a.177 Cl F F F F F CF₃ F I.a.178 F Cl F F F F CF₃ F I.a.179 CN F F F F F CF₃ F I.a.180 F CN F F F F CF₃ F I.a.181 H F F F F F CF₃ F I.a.182 F F Br F F F CF₃ F I.a.183 F F C≡CH F F F CF₃ F I.a.184 CF₃ Cl H H F F CF₃ F I.a.185 F F I F F F CF₃ F I.a.186 F H H H F F CH₃ F I.a.187 Cl H H H F F CH₃ F I.a.188 Br H H H F F CH₃ F I.a.189 CN H H H F F CH₃ F I.a.190 CH₃ H H H F F CH₃ F I.a.191 F H H F F F CH₃ F I.a.192 Cl H H F F F CH₃ F I.a.193 F H H Cl F F CH₃ F I.a.194 Cl H H F F F CH₃ F I.a.195 CN H H F F F CH₃ F I.a.196 F H H CN F F CH₃ F I.a.197 CN H H F F F CH₃ F I.a.198 F H F H F F CH₃ F I.a.199 Cl H F H F F CH₃ F I.a.200 CN H F H F F CH₃ F I.a.201 F F F H F F CH₃ F I.a.202 Cl F F H F F CH₃ F I.a.203 F Cl F H F F CH₃ F I.a.204 Cl F F H F F CH₃ F I.a.205 CN F F H F F CH₃ F I.a.206 F CN F H F F CH₃ F I.a.207 CN F F H F F CH₃ F I.a.208 F F H F F F CH₃ F I.a.209 Cl F H F F F CH₃ F I.a.210 F Cl H F F F CH₃ F I.a.211 CN F H F F F CH₃ F I.a.212 F CN H F F F CH₃ F I.a.213 F F F F F F CH₃ F I.a.214 Cl F F F F F CH₃ F I.a.215 F Cl F F F F CH₃ F I.a.216 CN F F F F F CH₃ F I.a.217 F CN F F F F CH₃ F I.a.218 H F F F F F CH₃ F I.a.219 F F Br F F F CH₃ F I.a.220 F F C≡CH F F F CH₃ F I.a.221 CF₃ Cl H H F F CH₃ F I.a.222 F F I F F F CH₃ F I.a.223 F H H H F F CH₃ H I.a.224 Cl H H H F F CH₃ H I.a.225 Br H H H F F CH₃ H I.a.226 CN H H H F F CH₃ H I.a.227 CH₃ H H H F F CH₃ H I.a.228 F H H F F F CH₃ H I.a.229 Cl H H F F F CH₃ H I.a.230 F H H Cl F F CH₃ H I.a.231 Cl H H F F F CH₃ H I.a.232 CN H H F F F CH₃ H I.a.233 F H H CN F F CH₃ H I.a.234 CN H H F F F CH₃ H I.a.235 F H F H F F CH₃ H I.a.236 Cl H F H F F CH₃ H I.a.237 CN H F H F F CH₃ H I.a.238 F F F H F F CH₃ H I.a.239 Cl F F H F F CH₃ H I.a.240 F Cl F H F F CH₃ H I.a.241 Cl F F H F F CH₃ H I.a.242 CN F F H F F CH₃ H I.a.243 F CN F H F F CH₃ H I.a.244 CN F F H F F CH₃ H I.a.245 F F H F F F CH₃ H I.a.246 Cl F H F F F CH₃ H I.a.247 F Cl H F F F CH₃ H I.a.248 CN F H F F F CH₃ H I.a.249 F CN H F F F CH₃ H I.a.250 F F F F F F CH₃ H I.a.251 Cl F F F F F CH₃ H I.a.252 F Cl F F F F CH₃ H I.a.253 CN F F F F F CH₃ H I.a.254 F CN F F F F CH₃ H I.a.255 H F F F F F CH₃ H I.a.256 F F Br F F F CH₃ H I.a.257 F F C≡CH F F F CH₃ H I.a.258 CF₃ Cl H H F F CH₃ H I.a.259 F F I F F F CH₃ H I.a.260 F H H H F F CH₃ CH₃ I.a.261 Cl H H H F F CH₃ CH₃ I.a.262 Br H H H F F CH₃ CH₃ I.a.263 CN H H H F F CH₃ CH₃ I.a.264 CH₃ H H H F F CH₃ CH₃ I.a.265 F H H F F F CH₃ CH₃ I.a.266 Cl H H F F F CH₃ CH₃ I.a.267 F H H Cl F F CH₃ CH₃ I.a.268 Cl H H F F F CH₃ CH₃ I.a.269 CN H H F F F CH₃ CH₃ I.a.270 F H H CN F F CH₃ CH₃ I.a.271 CN H H F F F CH₃ CH₃ I.a.272 F H F H F F CH₃ CH₃ I.a.273 Cl H F H F F CH₃ CH₃ I.a.274 CN H F H F F CH₃ CH₃ I.a.275 F F F H F F CH₃ CH₃ I.a.276 Cl F F H F F CH₃ CH₃ I.a.277 F Cl F H F F CH₃ CH₃ I.a.278 Cl F F H F F CH₃ CH₃ I.a.279 CN F F H F F CH₃ CH₃ I.a.280 F CN F H F F CH₃ CH₃ I.a.281 CN F F H F F CH₃ CH₃ I.a.282 F F H F F F CH₃ CH₃ I.a.283 Cl F H F F F CH₃ CH₃ I.a.284 F Cl H F F F CH₃ CH₃ I.a.285 CN F H F F F CH₃ CH₃ I.a.286 F CN H F F F CH₃ CH₃ I.a.287 F F F F F F CH₃ CH₃ I.a.288 Cl F F F F F CH₃ CH₃ I.a.289 F Cl F F F F CH₃ CH₃ I.a.290 CN F F F F F CH₃ CH₃ I.a.291 F CN F F F F CH₃ CH₃ I.a.292 H F F F F F CH₃ CH₃ I.a.293 F F Br F F F CH₃ CH₃ I.a.294 F F C≡CH F F F CH₃ CH₃ I.a.295 CF₃ Cl H H F F CH₃ CH₃ I.a.296 F F I F F F CH₃ CH₃ I.a.297 F H H H F Cl CH₃ CH₃ I.a.298 Cl H H H F Cl CH₃ CH₃ I.a.299 Br H H H F Cl CH₃ CH₃ I.a.300 CN H H H F Cl CH₃ CH₃ I.a.301 CH₃ H H H F Cl CH₃ CH₃ I.a.302 F H H F F Cl CH₃ CH₃ I.a.303 Cl H H F F Cl CH₃ CH₃ I.a.304 F H H Cl F Cl CH₃ CH₃ I.a.305 Cl H H F F Cl CH₃ CH₃ I.a.306 CN H H F F Cl CH₃ CH₃ I.a.307 F H H CN F Cl CH₃ CH₃ I.a.308 CN H H F F Cl CH₃ CH₃ I.a.309 F H F H F Cl CH₃ CH₃ I.a.310 Cl H F H F Cl CH₃ CH₃ I.a.311 CN H F H F Cl CH₃ CH₃ I.a.312 F F F H F Cl CH₃ CH₃ I.a.313 Cl F F H F Cl CH₃ CH₃ I.a.314 F Cl F H F Cl CH₃ CH₃ I.a.315 Cl F F H F Cl CH₃ CH₃ I.a.316 CN F F H F Cl CH₃ CH₃ I.a.317 F CN F H F Cl CH₃ CH₃ I.a.318 CN F F H F Cl CH₃ CH₃ I.a.319 F F H F F Cl CH₃ CH₃ I.a.320 Cl F H F F Cl CH₃ CH₃ I.a.321 F Cl H F F Cl CH₃ CH₃ I.a.322 CN F H F F Cl CH₃ CH₃ I.a.323 F CN H F F Cl CH₃ CH₃ I.a.324 F F F F F Cl CH₃ CH₃ I.a.325 Cl F F F F Cl CH₃ CH₃ I.a.326 F Cl F F F Cl CH₃ CH₃ I.a.327 CN F F F F Cl CH₃ CH₃ I.a.328 F CN F F F Cl CH₃ CH₃ I.a.329 H F F F F Cl CH₃ CH₃ I.a.330 F F Br F F Cl CH₃ CH₃ I.a.331 F F C≡CH F F Cl CH₃ CH₃ I.a.332 CF₃ Cl H H F Cl CH₃ CH₃ I.a.333 F F I F F Cl CH₃ CH₃ I.a.334 F H H H F F C₂H₅ CH₃ I.a.335 Cl H H H F F C₂H₅ CH₃ I.a.336 Br H H H F F C₂H₅ CH₃ I.a.337 CN H H H F F C₂H₅ CH₃ I.a.338 CH₃ H H H F F C₂H₅ CH₃ I.a.339 F H H F F F C₂H₅ CH₃ I.a.340 Cl H H F F F C₂H₅ CH₃ I.a.341 F H H Cl F F C₂H₅ CH₃ I.a.342 Cl H H F F F C₂H₅ CH₃ I.a.343 CN H H F F F C₂H₅ CH₃ I.a.344 F H H CN F F C₂H₅ CH₃ I.a.345 CN H H F F F C₂H₅ CH₃ I.a.346 F H F H F F C₂H₅ CH₃ I.a.347 Cl H F H F F C₂H₅ CH₃ I.a.348 CN H F H F F C₂H₅ CH₃ I.a.349 F F F H F F C₂H₅ CH₃ I.a.350 Cl F F H F F C₂H₅ CH₃ I.a.351 F Cl F H F F C₂H₅ CH₃ I.a.352 Cl F F H F F C₂H₅ CH₃ I.a.353 CN F F H F F C₂H₅ CH₃ I.a.354 F CN F H F F C₂H₅ CH₃ I.a.355 CN F F H F F C₂H₅ CH₃ I.a.356 F F H F F F C₂H₅ CH₃ I.a.357 Cl F H F F F C₂H₅ CH₃ I.a.358 F Cl H F F F C₂H₅ CH₃ I.a.359 CN F H F F F C₂H₅ CH₃ I.a.360 F CN H F F F C₂H₅ CH₃ I.a.361 F F F F F F C₂H₅ CH₃ I.a.362 Cl F F F F F C₂H₅ CH₃ I.a.363 F Cl F F F F C₂H₅ CH₃ I.a.364 CN F F F F F C₂H₅ CH₃ I.a.365 F CN F F F F C₂H₅ CH₃ I.a.366 H F F F F F C₂H₅ CH₃ I.a.367 F F Br F F F C₂H₅ CH₃ I.a.368 F F C≡CH F F F C₂H₅ CH₃ I.a.369 CF₃ Cl H H F F C₂H₅ CH₃ I.a.370 F F I F F F C₂H₅ CH₃ I.a.371 F H H H F F C₂H₅ C₂H₅ I.a.372 Cl H H H F F C₂H₅ C₂H₅ I.a.373 Br H H H F F C₂H₅ C₂H₅ I.a.374 CN H H H F F C₂H₅ C₂H₅ I.a.375 CH₃ H H H F F C₂H₅ C₂H₅ I.a.376 F H H F F F C₂H₅ C₂H₅ I.a.377 Cl H H F F F C₂H₅ C₂H₅ I.a.378 F H H Cl F F C₂H₅ C₂H₅ I.a.379 Cl H H F F F C₂H₅ C₂H₅ I.a.380 CN H H F F F C₂H₅ C₂H₅ I.a.381 F H H CN F F C₂H₅ C₂H₅ I.a.382 CN H H F F F C₂H₅ C₂H₅ I.a.383 F H F H F F C₂H₅ C₂H₅ I.a.384 Cl H F H F F C₂H₅ C₂H₅ I.a.385 CN H F H F F C₂H₅ C₂H₅ I.a.386 F F F H F F C₂H₅ C₂H₅ I.a.387 Cl F F H F F C₂H₅ C₂H₅ I.a.388 F Cl F H F F C₂H₅ C₂H₅ I.a.389 Cl F F H F F C₂H₅ C₂H₅ I.a.390 CN F F H F F C₂H₅ C₂H₅ I.a.391 F CN F H F F C₂H₅ C₂H₅ I.a.392 CN F F H F F C₂H₅ C₂H₅ I.a.393 F F H F F F C₂H₅ C₂H₅ I.a.394 Cl F H F F F C₂H₅ C₂H₅ I.a.395 F Cl H F F F C₂H₅ C₂H₅ I.a.396 CN F H F F F C₂H₅ C₂H₅ I.a.397 F CN H F F F C₂H₅ C₂H₅ I.a.398 F F F F F F C₂H₅ C₂H₅ I.a.399 Cl F F F F F C₂H₅ C₂H₅ I.a.400 F Cl F F F F C₂H₅ C₂H₅ I.a.401 CN F F F F F C₂H₅ C₂H₅ I.a.402 F CN F F F F C₂H₅ C₂H₅ I.a.403 H F F F F F C₂H₅ C₂H₅ I.a.404 F F Br F F F C₂H₅ C₂H₅ I.a.405 F F C≡CH F F F C₂H₅ C₂H₅ I.a.406 CF₃ Cl H H F F C₂H₅ C₂H₅ I.a.407 F F I F F F C₂H₅ C₂H₅ I.a.408 F H H H F H —(CH₂)₂— I.a.409 Cl H H H F H —(CH₂)₂— I.a.410 Br H H H F H —(CH₂)₂— I.a.411 CN H H H F H —(CH₂)₂— I.a.412 CH₃ H H H F H —(CH₂)₂— I.a.413 F H H F F H —(CH₂)₂— I.a.414 Cl H H F F H —(CH₂)₂— I.a.415 F H H Cl F H —(CH₂)₂— I.a.416 Cl H H F F H —(CH₂)₂— I.a.417 CN H H F F H —(CH₂)₂— I.a.418 F H H CN F H —(CH₂)₂— I.a.419 CN H H F F H —(CH₂)₂— I.a.420 F H F H F H —(CH₂)₂— I.a.421 Cl H F H F H —(CH₂)₂— I.a.422 CN H F H F H —(CH₂)₂— I.a.423 F F F H F H —(CH₂)₂— I.a.424 Cl F F H F H —(CH₂)₂— I.a.425 F Cl F H F H —(CH₂)₂— I.a.426 Cl F F H F H —(CH₂)₂— I.a.427 CN F F H F H —(CH₂)₂— I.a.428 F CN F H F H —(CH₂)₂— I.a.429 CN F F H F H —(CH₂)₂— I.a.430 F F H F F H —(CH₂)₂— I.a.431 Cl F H F F H —(CH₂)₂— I.a.432 F Cl H F F H —(CH₂)₂— I.a.433 CN F H F F H —(CH₂)₂— I.a.434 F CN H F F H —(CH₂)₂— I.a.435 F F F F F H —(CH₂)₂— I.a.436 Cl F F F F H —(CH₂)₂— I.a.437 F Cl F F F H —(CH₂)₂— I.a.438 CN F F F F H —(CH₂)₂— I.a.439 F CN F F F H —(CH₂)₂— I.a.440 H F F F F H —(CH₂)₂— I.a.441 F F Br F F H —(CH₂)₂— I.a.442 F F C≡CH F F H —(CH₂)₂— I.a.443 CF₃ Cl H H F H —(CH₂)₂— I.a.444 F F I F F H —(CH₂)₂— I.a.445 F H H H F H —(CH₂)₃— I.a.446 Cl H H H F H —(CH₂)₃— I.a.447 Br H H H F H —(CH₂)₃— I.a.448 CN H H H F H —(CH₂)₃— I.a.449 CH₃ H H H F H —(CH₂)₃— I.a.450 F H H F F H —(CH₂)₃— I.a.451 Cl H H F F H —(CH₂)₃— I.a.452 F H H Cl F H —(CH₂)₃— I.a.453 Cl H H F F H —(CH₂)₃— I.a.454 CN H H F F H —(CH₂)₃— I.a.455 F H H CN F H —(CH₂)₃— I.a.456 CN H H F F H —(CH₂)₃— I.a.457 F H F H F H —(CH₂)₃— I.a.458 Cl H F H F H —(CH₂)₃— I.a.459 CN H F H F H —(CH₂)₃— I.a.460 F F F H F H —(CH₂)₃— I.a.461 Cl F F H F H —(CH₂)₃— I.a.462 F Cl F H F H —(CH₂)₃— I.a.463 Cl F F H F H —(CH₂)₃— I.a.464 CN F F H F H —(CH₂)₃— I.a.465 F CN F H F H —(CH₂)₃— I.a.466 CN F F H F H —(CH₂)₃— I.a.467 F F H F F H —(CH₂)₃— I.a.468 Cl F H F F H —(CH₂)₃— I.a.469 F Cl H F F H —(CH₂)₃— I.a.470 CN F H F F H —(CH₂)₃— I.a.471 F CN H F F H —(CH₂)₃— I.a.472 F F F F F H —(CH₂)₃— I.a.473 Cl F F F F H —(CH₂)₃— I.a.474 F Cl F F F H —(CH₂)₃— I.a.475 CN F F F F H —(CH₂)₃— I.a.476 F CN F F F H —(CH₂)₃— I.a.477 H F F F F H —(CH₂)₃— I.a.478 F F Br F F H —(CH₂)₃— I.a.479 F F C≡CH F F H —(CH₂)₃— I.a.480 CF₃ Cl H H F H —(CH₂)₃— I.a.481 F F I F F H —(CH₂)₃— I.a.482 F H H H F H —(CH₂)₄— I.a.483 Cl H H H F H —(CH₂)₄— I.a.484 Br H H H F H —(CH₂)₄— I.a.485 CN H H H F H —(CH₂)₄— I.a.486 CH₃ H H H F H —(CH₂)₄— I.a.487 F H H F F H —(CH₂)₄— I.a.488 Cl H H F F H —(CH₂)₄— I.a.489 F H H Cl F H —(CH₂)₄— I.a.490 Cl H H F F H —(CH₂)₄— I.a.491 CN H H F F H —(CH₂)₄— I.a.492 F H H CN F H —(CH₂)₄— I.a.493 CN H H F F H —(CH₂)₄— I.a.494 F H F H F H —(CH₂)₄— I.a.495 Cl H F H F H —(CH₂)₄— I.a.496 CN H F H F H —(CH₂)₄— I.a.497 F F F H F H —(CH₂)₄— I.a.498 Cl F F H F H —(CH₂)₄— I.a.499 F Cl F H F H —(CH₂)₄— I.a.500 Cl F F H F H —(CH₂)₄— I.a.501 CN F F H F H —(CH₂)₄— I.a.502 F CN F H F H —(CH₂)₄— I.a.503 CN F F H F H —(CH₂)₄— I.a.504 F F H F F H —(CH₂)₄— I.a.505 Cl F H F F H —(CH₂)₄— I.a.506 F Cl H F F H —(CH₂)₄— I.a.507 CN F H F F H —(CH₂)₄— I.a.508 F CN H F F H —(CH₂)₄— I.a.509 F F F F F H —(CH₂)₄— I.a.510 Cl F F F F H —(CH₂)₄— I.a.511 F Cl F F F H —(CH₂)₄— I.a.512 CN F F F F H —(CH₂)₄— I.a.513 F CN F F F H —(CH₂)₄— I.a.514 H F F F F H —(CH₂)₄— I.a.515 F F Br F F H —(CH₂)₄— I.a.516 F F C≡CH F F H —(CH₂)₄— I.a.517 CF₃ Cl H H F H —(CH₂)₄— I.a.518 F F I F F H —(CH₂)₄— I.a.519 F H H H F H —(CH₂)₅— I.a.520 Cl H H H F H —(CH₂)₅— I.a.521 Br H H H F H —(CH₂)₅— I.a.522 CN H H H F H —(CH₂)₅— I.a.523 CH₃ H H H F H —(CH₂)₅— I.a.524 F H H F F H —(CH₂)₅— I.a.525 Cl H H F F H —(CH₂)₅— I.a.526 F H H Cl F H —(CH₂)₅— I.a.527 Cl H H F F H —(CH₂)₅— I.a.528 CN H H F F H —(CH₂)₅— I.a.529 F H H CN F H —(CH₂)₅— I.a.530 CN H H F F H —(CH₂)₅— I.a.531 F H F H F H —(CH₂)₅— I.a.532 Cl H F H F H —(CH₂)₅— I.a.533 CN H F H F H —(CH₂)₅— I.a.534 F F F H F H —(CH₂)₅— I.a.535 Cl F F H F H —(CH₂)₅— I.a.536 F Cl F H F H —(CH₂)₅— I.a.537 Cl F F H F H —(CH₂)₅— I.a.538 CN F F H F H —(CH₂)₅— I.a.539 F CN F H F H —(CH₂)₅— I.a.540 CN F F H F H —(CH₂)₅— I.a.541 F F H F F H —(CH₂)₅— I.a.542 Cl F H F F H —(CH₂)₅— I.a.543 F Cl H F F H —(CH₂)₅— I.a.544 CN F H F F H —(CH₂)₅— I.a.545 F CN H F F H —(CH₂)₅— I.a.546 F F F F F H —(CH₂)₅— I.a.547 Cl F F F F H —(CH₂)₅— I.a.548 F Cl F F F H —(CH₂)₅— I.a.549 CN F F F F H —(CH₂)₅— I.a.550 F CN F F F H —(CH₂)₅— I.a.551 H F F F F H —(CH₂)₅— I.a.552 F F Br F F H —(CH₂)₅— I.a.553 F F C≡CH F F H —(CH₂)₅— I.a.554 CF₃ Cl H H F H —(CH₂)₅— I.a.555 F F I F F H —(CH₂)₅— I.a.556 F H H H F CH₃ —(CH₂)₂— I.a.557 Cl H H H F CH₃ —(CH₂)₂— I.a.558 Br H H H F CH₃ —(CH₂)₂— I.a.559 CN H H H F CH₃ —(CH₂)₂— I.a.560 CH₃ H H H F CH₃ —(CH₂)₂— I.a.561 F H H F F CH₃ —(CH₂)₂— I.a.562 Cl H H F F CH₃ —(CH₂)₂— I.a.563 F H H Cl F CH₃ —(CH₂)₂— I.a.564 Cl H H F F CH₃ —(CH₂)₂— I.a.565 CN H H F F CH₃ —(CH₂)₂— I.a.566 F H H CN F CH₃ —(CH₂)₂— I.a.567 CN H H F F CH₃ —(CH₂)₂— I.a.568 F H F H F CH₃ —(CH₂)₂— I.a.569 Cl H F H F CH₃ —(CH₂)₂— I.a.570 CN H F H F CH₃ —(CH₂)₂— I.a.571 F F F H F CH₃ —(CH₂)₂— I.a.572 Cl F F H F CH₃ —(CH₂)₂— I.a.573 F Cl F H F CH₃ —(CH₂)₂— I.a.574 Cl F F H F CH₃ —(CH₂)₂— I.a.575 CN F F H F CH₃ —(CH₂)₂— I.a.576 F CN F H F CH₃ —(CH₂)₂— I.a.577 CN F F H F CH₃ —(CH₂)₂— I.a.578 F F H F F CH₃ —(CH₂)₂— I.a.579 Cl F H F F CH₃ —(CH₂)₂— I.a.580 F Cl H F F CH₃ —(CH₂)₂— I.a.581 CN F H F F CH₃ —(CH₂)₂— I.a.582 F CN H F F CH₃ —(CH₂)₂— I.a.583 F F F F F CH₃ —(CH₂)₂— I.a.584 Cl F F F F CH₃ —(CH₂)₂— I.a.585 F Cl F F F CH₃ —(CH₂)₂— I.a.586 CN F F F F CH₃ —(CH₂)₂— I.a.587 F CN F F F CH₃ —(CH₂)₂— I.a.588 H F F F F CH₃ —(CH₂)₂— I.a.589 F F Br F F CH₃ —(CH₂)₂— I.a.590 F F C≡CH F F CH₃ —(CH₂)₂— I.a.591 CF₃ Cl H H F CH₃ —(CH₂)₂— I.a.592 F F I F F CH₃ —(CH₂)₂— I.a.593 F H H H F CH₃ —(CH₂)₃— I.a.594 Cl H H H F CH₃ —(CH₂)₃— I.a.595 Br H H H F CH₃ —(CH₂)₃— I.a.596 CN H H H F CH₃ —(CH₂)₃— I.a.597 CH₃ H H H F CH₃ —(CH₂)₃— I.a.598 F H H F F CH₃ —(CH₂)₃— I.a.599 Cl H H F F CH₃ —(CH₂)₃— I.a.600 F H H Cl F CH₃ —(CH₂)₃— I.a.601 Cl H H F F CH₃ —(CH₂)₃— I.a.602 CN H H F F CH₃ —(CH₂)₃— I.a.603 F H H CN F CH₃ —(CH₂)₃— I.a.604 CN H H F F CH₃ —(CH₂)₃— I.a.605 F H F H F CH₃ —(CH₂)₃— I.a.606 Cl H F H F CH₃ —(CH₂)₃— I.a.607 CN H F H F CH₃ —(CH₂)₃— I.a.608 F F F H F CH₃ —(CH₂)₃— I.a.609 Cl F F H F CH₃ —(CH₂)₃— I.a.610 F Cl F H F CH₃ —(CH₂)₃— I.a.611 Cl F F H F CH₃ —(CH₂)₃— I.a.612 CN F F H F CH₃ —(CH₂)₃— I.a.613 F CN F H F CH₃ —(CH₂)₃— I.a.614 CN F F H F CH₃ —(CH₂)₃— I.a.615 F F H F F CH₃ —(CH₂)₃— I.a.616 Cl F H F F CH₃ —(CH₂)₃— I.a.617 F Cl H F F CH₃ —(CH₂)₃— I.a.618 CN F H F F CH₃ —(CH₂)₃— I.a.619 F CN H F F CH₃ —(CH₂)₃— I.a.620 F F F F F CH₃ —(CH₂)₃— I.a.621 Cl F F F F CH₃ —(CH₂)₃— I.a.622 F Cl F F F CH₃ —(CH₂)₃— I.a.623 CN F F F F CH₃ —(CH₂)₃— I.a.624 F CN F F F CH₃ —(CH₂)₃— I.a.625 H F F F F CH₃ —(CH₂)₃— I.a.626 F F Br F F CH₃ —(CH₂)₃— I.a.627 F F C≡CH F F CH₃ —(CH₂)₃— I.a.628 CF₃ Cl H H F CH₃ —(CH₂)₃— I.a.629 F F I F F CH₃ —(CH₂)₃— I.a.630 F H H H F CH₃ —(CH₂)₄— I.a.631 Cl H H H F CH₃ —(CH₂)₄— I.a.632 Br H H H F CH₃ —(CH₂)₄— I.a.633 CN H H H F CH₃ —(CH₂)₄— I.a.634 CH₃ H H H F CH₃ —(CH₂)₄— I.a.635 F H H F F CH₃ —(CH₂)₄— I.a.636 Cl H H F F CH₃ —(CH₂)₄— I.a.637 F H H Cl F CH₃ —(CH₂)₄— I.a.638 Cl H H F F CH₃ —(CH₂)₄— I.a.639 CN H H F F CH₃ —(CH₂)₄— I.a.640 F H H CN F CH₃ —(CH₂)₄— I.a.641 CN H H F F CH₃ —(CH₂)₄— I.a.642 F H F H F CH₃ —(CH₂)₄— I.a.643 Cl H F H F CH₃ —(CH₂)₄— I.a.644 CN H F H F CH₃ —(CH₂)₄— I.a.645 F F F H F CH₃ —(CH₂)₄— I.a.646 Cl F F H F CH₃ —(CH₂)₄— I.a.647 F Cl F H F CH₃ —(CH₂)₄— I.a.648 Cl F F H F CH₃ —(CH₂)₄— I.a.649 CN F F H F CH₃ —(CH₂)₄— I.a.650 F CN F H F CH₃ —(CH₂)₄— I.a.651 CN F F H F CH₃ —(CH₂)₄— I.a.652 F F H F F CH₃ —(CH₂)₄— I.a.653 Cl F H F F CH₃ —(CH₂)₄— I.a.654 F Cl H F F CH₃ —(CH₂)₄— I.a.655 CN F H F F CH₃ —(CH₂)₄— I.a.656 F CN H F F CH₃ —(CH₂)₄— I.a.657 F F F F F CH₃ —(CH₂)₄— I.a.658 Cl F F F F CH₃ —(CH₂)₄— I.a.659 F Cl F F F CH₃ —(CH₂)₄— I.a.660 CN F F F F CH₃ —(CH₂)₄— I.a.661 F CN F F F CH₃ —(CH₂)₄— I.a.662 H F F F F CH₃ —(CH₂)₄— I.a.663 F F Br F F CH₃ —(CH₂)₄— I.a.664 F F C≡CH F F CH₃ —(CH₂)₄— I.a.665 CF₃ Cl H H F CH₃ —(CH₂)₄— I.a.666 F F I F F CH₃ —(CH₂)₄— I.a.667 F H H H F CH₃ —(CH₂)₅— I.a.668 Cl H H H F CH₃ —(CH₂)₅— I.a.669 Br H H H F CH₃ —(CH₂)₅— I.a.670 CN H H H F CH₃ —(CH₂)₅— I.a.671 CH₃ H H H F CH₃ —(CH₂)₅— I.a.672 F H H F F CH₃ —(CH₂)₅— I.a.673 Cl H H F F CH₃ —(CH₂)₅— I.a.674 F H H Cl F CH₃ —(CH₂)₅— I.a.675 Cl H H F F CH₃ —(CH₂)₅— I.a.676 CN H H F F CH₃ —(CH₂)₅— I.a.677 F H H CN F CH₃ —(CH₂)₅— I.a.678 CN H H F F CH₃ —(CH₂)₅— I.a.679 F H F H F CH₃ —(CH₂)₅— I.a.680 Cl H F H F CH₃ —(CH₂)₅— I.a.681 CN H F H F CH₃ —(CH₂)₅— I.a.682 F F F H F CH₃ —(CH₂)₅— I.a.683 Cl F F H F CH₃ —(CH₂)₅— I.a.684 F Cl F H F CH₃ —(CH₂)₅— I.a.685 Cl F F H F CH₃ —(CH₂)₅— I.a.686 CN F F H F CH₃ —(CH₂)₅— I.a.687 F CN F H F CH₃ —(CH₂)₅— I.a.688 CN F F H F CH₃ —(CH₂)₅— I.a.689 F F H F F CH₃ —(CH₂)₅— I.a.690 Cl F H F F CH₃ —(CH₂)₅— I.a.691 F Cl H F F CH₃ —(CH₂)₅— I.a.692 CN F H F F CH₃ —(CH₂)₅— I.a.693 F CN H F F CH₃ —(CH₂)₅— I.a.694 F F F F F CH₃ —(CH₂)₅— I.a.695 Cl F F F F CH₃ —(CH₂)₅— I.a.696 F Cl F F F CH₃ —(CH₂)₅— I.a.697 CN F F F F CH₃ —(CH₂)₅— I.a.698 F CN F F F CH₃ —(CH₂)₅— I.a.699 H F F F F CH₃ —(CH₂)₅— I.a.700 F F Br F F CH₃ —(CH₂)₅— I.a.701 F F C≡CH F F CH₃ —(CH₂)₅— I.a.702 CF₃ Cl H H F CH₃ —(CH₂)₅— I.a.703 F F I F F CH₃ —(CH₂)₅— I.a.704 F H H H F F —(CH₂)₂— I.a.705 Cl H H H F F —(CH₂)₂— I.a.706 Br H H H F F —(CH₂)₂— I.a.707 CN H H H F F —(CH₂)₂— I.a.708 CH₃ H H H F F —(CH₂)₂— I.a.709 F H H F F F —(CH₂)₂— I.a.710 Cl H H F F F —(CH₂)₂— I.a.711 F H H Cl F F —(CH₂)₂— I.a.712 Cl H H F F F —(CH₂)₂— I.a.713 CN H H F F F —(CH₂)₂— I.a.714 F H H CN F F —(CH₂)₂— I.a.715 CN H H F F F —(CH₂)₂— I.a.716 F H F H F F —(CH₂)₂— I.a.717 Cl H F H F F —(CH₂)₂— I.a.718 CN H F H F F —(CH₂)₂— I.a.719 F F F H F F —(CH₂)₂— I.a.720 Cl F F H F F —(CH₂)₂— I.a.721 F Cl F H F F —(CH₂)₂— I.a.722 Cl F F H F F —(CH₂)₂— I.a.723 CN F F H F F —(CH₂)₂— I.a.724 F CN F H F F —(CH₂)₂— I.a.725 CN F F H F F —(CH₂)₂— I.a.726 F F H F F F —(CH₂)₂— I.a.727 Cl F H F F F —(CH₂)₂— I.a.728 F Cl H F F F —(CH₂)₂— I.a.729 CN F H F F F —(CH₂)₂— I.a.730 F CN H F F F —(CH₂)₂— I.a.731 F F F F F F —(CH₂)₂— I.a.732 Cl F F F F F —(CH₂)₂— I.a.733 F Cl F F F F —(CH₂)₂— I.a.734 CN F F F F F —(CH₂)₂— I.a.735 F CN F F F F —(CH₂)₂— I.a.736 H F F F F F —(CH₂)₂— I.a.737 F F Br F F F —(CH₂)₂— I.a.738 F F C≡CH F F F —(CH₂)₂— I.a.739 CF₃ Cl H H F F —(CH₂)₂— I.a.740 F F I F F F —(CH₂)₂— I.a.741 F H H H F F —(CH₂)₃— I.a.742 Cl H H H F F —(CH₂)₃— I.a.743 Br H H H F F —(CH₂)₃— I.a.744 CN H H H F F —(CH₂)₃— I.a.745 CH₃ H H H F F —(CH₂)₃— I.a.746 F H H F F F —(CH₂)₃— I.a.747 Cl H H F F F —(CH₂)₃— I.a.748 F H H Cl F F —(CH₂)₃— I.a.749 Cl H H F F F —(CH₂)₃— I.a.750 CN H H F F F —(CH₂)₃— I.a.751 F H H CN F F —(CH₂)₃— I.a.752 CN H H F F F —(CH₂)₃— I.a.753 F H F H F F —(CH₂)₃— I.a.754 Cl H F H F F —(CH₂)₃— I.a.755 CN H F H F F —(CH₂)₃— I.a.756 F F F H F F —(CH₂)₃— I.a.757 Cl F F H F F —(CH₂)₃— I.a.758 F Cl F H F F —(CH₂)₃— I.a.759 Cl F F H F F —(CH₂)₃— I.a.760 CN F F H F F —(CH₂)₃— I.a.761 F CN F H F F —(CH₂)₃— I.a.762 CN F F H F F —(CH₂)₃— I.a.763 F F H F F F —(CH₂)₃— I.a.764 Cl F H F F F —(CH₂)₃— I.a.765 F Cl H F F F —(CH₂)₃— I.a.766 CN F H F F F —(CH₂)₃— I.a.767 F CN H F F F —(CH₂)₃— I.a.768 F F F F F F —(CH₂)₃— I.a.769 Cl F F F F F —(CH₂)₃— I.a.770 F Cl F F F F —(CH₂)₃— I.a.771 CN F F F F F —(CH₂)₃— I.a.772 F CN F F F F —(CH₂)₃— I.a.773 H F F F F F —(CH₂)₃— I.a.774 F F Br F F F —(CH₂)₃— I.a.775 F F C≡CH F F F —(CH₂)₃— I.a.776 CF₃ Cl H H F F —(CH₂)₃— I.a.777 F F I F F F —(CH₂)₃— I.a.778 F H H H F F —(CH₂)₄— I.a.779 Cl H H H F F —(CH₂)₄— I.a.780 Br H H H F F —(CH₂)₄— I.a.781 CN H H H F F —(CH₂)₄— I.a.782 CH₃ H H H F F —(CH₂)₄— I.a.783 F H H F F F —(CH₂)₄— I.a.784 Cl H H F F F —(CH₂)₄— I.a.785 F H H Cl F F —(CH₂)₄— I.a.786 Cl H H F F F —(CH₂)₄— I.a.787 CN H H F F F —(CH₂)₄— I.a.788 F H H CN F F —(CH₂)₄— I.a.789 CN H H F F F —(CH₂)₄— I.a.790 F H F H F F —(CH₂)₄— I.a.791 Cl H F H F F —(CH₂)₄— I.a.792 CN H F H F F —(CH₂)₄— I.a.793 F F F H F F —(CH₂)₄— I.a.794 Cl F F H F F —(CH₂)₄— I.a.795 F Cl F H F F —(CH₂)₄— I.a.796 Cl F F H F F —(CH₂)₄— I.a.797 CN F F H F F —(CH₂)₄— I.a.798 F CN F H F F —(CH₂)₄— I.a.799 CN F F H F F —(CH₂)₄— I.a.800 F F H F F F —(CH₂)₄— I.a.801 Cl F H F F F —(CH₂)₄— I.a.802 F Cl H F F F —(CH₂)₄— I.a.803 CN F H F F F —(CH₂)₄— I.a.804 F CN H F F F —(CH₂)₄— I.a.805 F F F F F F —(CH₂)₄— I.a.806 Cl F F F F F —(CH₂)₄— I.a.807 F Cl F F F F —(CH₂)₄— I.a.808 CN F F F F F —(CH₂)₄— I.a.809 F CN F F F F —(CH₂)₄— I.a.810 H F F F F F —(CH₂)₄— I.a.811 F F Br F F F —(CH₂)₄— I.a.812 F F C≡CH F F F —(CH₂)₄— I.a.813 CF₃ Cl H H F F —(CH₂)₄— I.a.814 F F I F F F —(CH₂)₄— I.a.815 F H H H F F —(CH₂)₅— I.a.816 Cl H H H F F —(CH₂)₅— I.a.817 Br H H H F F —(CH₂)₅— I.a.818 CN H H H F F —(CH₂)₅— I.a.819 CH₃ H H H F F —(CH₂)₅— I.a.820 F H H F F F —(CH₂)₅— I.a.821 Cl H H F F F —(CH₂)₅— I.a.822 F H H Cl F F —(CH₂)₅— I.a.823 Cl H H F F F —(CH₂)₅— I.a.824 CN H H F F F —(CH₂)₅— I.a.825 F H H CN F F —(CH₂)₅— I.a.826 CN H H F F F —(CH₂)₅— I.a.827 F H F H F F —(CH₂)₅— I.a.828 Cl H F H F F —(CH₂)₅— I.a.829 CN H F H F F —(CH₂)₅— I.a.830 F F F H F F —(CH₂)₅— I.a.831 Cl F F H F F —(CH₂)₅— I.a.832 F Cl F H F F —(CH₂)₅— I.a.833 Cl F F H F F —(CH₂)₅— I.a.834 CN F F H F F —(CH₂)₅— I.a.835 F CN F H F F —(CH₂)₅— I.a.836 CN F F H F F —(CH₂)₅— I.a.837 F F H F F F —(CH₂)₅— I.a.838 Cl F H F F F —(CH₂)₅— I.a.839 F Cl H F F F —(CH₂)₅— I.a.840 CN F H F F F —(CH₂)₅— I.a.841 F CN H F F F —(CH₂)₅— I.a.842 F F F F F F —(CH₂)₅— I.a.843 Cl F F F F F —(CH₂)₅— I.a.844 F Cl F F F F —(CH₂)₅— I.a.845 CN F F F F F —(CH₂)₅— I.a.846 F CN F F F F —(CH₂)₅— I.a.847 H F F F F F —(CH₂)₅— I.a.848 F F Br F F F —(CH₂)₅— I.a.849 F F C≡CH F F F —(CH₂)₅— I.a.850 CF₃ Cl H H F F —(CH₂)₅— I.a.851 F F I F F F —(CH₂)₅— I.a.852 F H H H F Cl —(CH₂)₂— I.a.853 Cl H H H F Cl —(CH₂)₂— I.a.854 Br H H H F Cl —(CH₂)₂— I.a.855 CN H H H F Cl —(CH₂)₂— I.a.856 CH₃ H H H F Cl —(CH₂)₂— I.a.857 F H H F F Cl —(CH₂)₂— I.a.858 Cl H H F F Cl —(CH₂)₂— I.a.859 F H H Cl F Cl —(CH₂)₂— I.a.860 Cl H H F F Cl —(CH₂)₂— I.a.861 CN H H F F Cl —(CH₂)₂— I.a.862 F H H CN F Cl —(CH₂)₂— I.a.863 CN H H F F Cl —(CH₂)₂— I.a.864 F H F H F Cl —(CH₂)₂— I.a.865 Cl H F H F Cl —(CH₂)₂— I.a.866 CN H F H F Cl —(CH₂)₂— I.a.867 F F F H F Cl —(CH₂)₂— I.a.868 Cl F F H F Cl —(CH₂)₂— I.a.869 F Cl F H F Cl —(CH₂)₂— I.a.870 Cl F F H F Cl —(CH₂)₂— I.a.871 CN F F H F Cl —(CH₂)₂— I.a.872 F CN F H F Cl —(CH₂)₂— I.a.873 CN F F H F Cl —(CH₂)₂— I.a.874 F F H F F Cl —(CH₂)₂— I.a.875 Cl F H F F Cl —(CH₂)₂— I.a.876 F Cl H F F Cl —(CH₂)₂— I.a.877 CN F H F F Cl —(CH₂)₂— I.a.878 F CN H F F Cl —(CH₂)₂— I.a.879 F F F F F Cl —(CH₂)₂— I.a.880 Cl F F F F Cl —(CH₂)₂— I.a.881 F Cl F F F Cl —(CH₂)₂— I.a.882 CN F F F F Cl —(CH₂)₂— I.a.883 F CN F F F Cl —(CH₂)₂— I.a.884 H F F F F Cl —(CH₂)₂— I.a.885 F F Br F F Cl —(CH₂)₂— I.a.886 F F C≡CH F F Cl —(CH₂)₂— I.a.887 CF₃ Cl H H F Cl —(CH₂)₂— I.a.888 F F I F F Cl —(CH₂)₂— I.a.889 F H H H F Cl —(CH₂)₃— I.a.890 Cl H H H F Cl —(CH₂)₃— I.a.891 Br H H H F Cl —(CH₂)₃— I.a.892 CN H H H F Cl —(CH₂)₃— I.a.893 CH₃ H H H F Cl —(CH₂)₃— I.a.894 F H H F F Cl —(CH₂)₃— I.a.895 Cl H H F F Cl —(CH₂)₃— I.a.896 F H H Cl F Cl —(CH₂)₃— I.a.897 Cl H H F F Cl —(CH₂)₃— I.a.898 CN H H F F Cl —(CH₂)₃— I.a.899 F H H CN F Cl —(CH₂)₃— I.a.900 CN H H F F Cl —(CH₂)₃— I.a.901 F H F H F Cl —(CH₂)₃— I.a.902 Cl H F H F Cl —(CH₂)₃— I.a.903 CN H F H F Cl —(CH₂)₃— I.a.904 F F F H F Cl —(CH₂)₃— I.a.905 Cl F F H F Cl —(CH₂)₃— I.a.906 F Cl F H F Cl —(CH₂)₃— I.a.907 Cl F F H F Cl —(CH₂)₃— I.a.908 CN F F H F Cl —(CH₂)₃— I.a.909 F CN F H F Cl —(CH₂)₃— I.a.910 CN F F H F Cl —(CH₂)₃— I.a.911 F F H F F Cl —(CH₂)₃— I.a.912 Cl F H F F Cl —(CH₂)₃— I.a.913 F Cl H F F Cl —(CH₂)₃— I.a.914 CN F H F F Cl —(CH₂)₃— I.a.915 F CN H F F Cl —(CH₂)₃— I.a.916 F F F F F Cl —(CH₂)₃— I.a.917 Cl F F F F Cl —(CH₂)₃— I.a.918 F Cl F F F Cl —(CH₂)₃— I.a.919 CN F F F F Cl —(CH₂)₃— I.a.920 F CN F F F Cl —(CH₂)₃— I.a.921 H F F F F Cl —(CH₂)₃— I.a.922 F F Br F F Cl —(CH₂)₃— I.a.923 F F C≡CH F F Cl —(CH₂)₃— I.a.924 CF₃ Cl H H F Cl —(CH₂)₃— I.a.925 F F I F F Cl —(CH₂)₃— I.a.926 F H H H F Cl —(CH₂)₄— I.a.927 Cl H H H F Cl —(CH₂)₄— I.a.928 Br H H H F Cl —(CH₂)₄— I.a.929 CN H H H F Cl —(CH₂)₄— I.a.930 CH₃ H H H F Cl —(CH₂)₄— I.a.931 F H H F F Cl —(CH₂)₄— I.a.932 Cl H H F F Cl —(CH₂)₄— I.a.933 F H H Cl F Cl —(CH₂)₄— I.a.934 Cl H H F F Cl —(CH₂)₄— I.a.935 CN H H F F Cl —(CH₂)₄— I.a.936 F H H CN F Cl —(CH₂)₄— I.a.937 CN H H F F Cl —(CH₂)₄— I.a.938 F H F H F Cl —(CH₂)₄— I.a.939 Cl H F H F Cl —(CH₂)₄— I.a.940 CN H F H F Cl —(CH₂)₄— I.a.941 F F F H F Cl —(CH₂)₄— I.a.942 Cl F F H F Cl —(CH₂)₄— I.a.943 F Cl F H F Cl —(CH₂)₄— I.a.944 Cl F F H F Cl —(CH₂)₄— I.a.945 CN F F H F Cl —(CH₂)₄— I.a.946 F CN F H F Cl —(CH₂)₄— I.a.947 CN F F H F Cl —(CH₂)₄— I.a.948 F F H F F Cl —(CH₂)₄— I.a.949 Cl F H F F Cl —(CH₂)₄— I.a.950 F Cl H F F Cl —(CH₂)₄— I.a.951 CN F H F F Cl —(CH₂)₄— I.a.952 F CN H F F Cl —(CH₂)₄— I.a.953 F F F F F Cl —(CH₂)₄— I.a.954 Cl F F F F Cl —(CH₂)₄— I.a.955 F Cl F F F Cl —(CH₂)₄— I.a.956 CN F F F F Cl —(CH₂)₄— I.a.957 F CN F F F Cl —(CH₂)₄— I.a.958 H F F F F Cl —(CH₂)₄— I.a.959 F F Br F F Cl —(CH₂)₄— I.a.960 F F C≡CH F F Cl —(CH₂)₄— I.a.961 CF₃ Cl H H F Cl —(CH₂)₄— I.a.962 F F I F F Cl —(CH₂)₄— I.a.963 F H H H F Cl —(CH₂)₅— I.a.964 Cl H H H F Cl —(CH₂)₅— I.a.965 Br H H H F Cl —(CH₂)₅— I.a.966 CN H H H F Cl —(CH₂)₅— I.a.967 CH₃ H H H F Cl —(CH₂)₅— I.a.968 F H H F F Cl —(CH₂)₅— I.a.969 Cl H H F F Cl —(CH₂)₅— I.a.970 F H H Cl F Cl —(CH₂)₅— I.a.971 Cl H H F F Cl —(CH₂)₅— I.a.972 CN H H F F Cl —(CH₂)₅— I.a.973 F H H CN F Cl —(CH₂)₅— I.a.974 CN H H F F Cl —(CH₂)₅— I.a.975 F H F H F Cl —(CH₂)₅— I.a.976 Cl H F H F Cl —(CH₂)₅— I.a.977 CN H F H F Cl —(CH₂)₅— I.a.978 F F F H F Cl —(CH₂)₅— I.a.979 Cl F F H F Cl —(CH₂)₅— I.a.980 F Cl F H F Cl —(CH₂)₅— I.a.981 Cl F F H F Cl —(CH₂)₅— I.a.982 CN F F H F Cl —(CH₂)₅— I.a.983 F CN F H F Cl —(CH₂)₅— I.a.984 CN F F H F Cl —(CH₂)₅— I.a.985 F F H F F Cl —(CH₂)₅— I.a.986 Cl F H F F Cl —(CH₂)₅— I.a.987 F Cl H F F Cl —(CH₂)₅— I.a.988 CN F H F F Cl —(CH₂)₅— I.a.989 F CN H F F Cl —(CH₂)₅— I.a.990 F F F F F Cl —(CH₂)₅— I.a.991 Cl F F F F Cl —(CH₂)₅— I.a.992 F Cl F F F Cl —(CH₂)₅— I.a.993 CN F F F F Cl —(CH₂)₅— I.a.994 F CN F F F Cl —(CH₂)₅— I.a.995 H F F F F Cl —(CH₂)₅— I.a.996 F F Br F F Cl —(CH₂)₅— I.a.997 F F C≡CH F F Cl —(CH₂)₅— I.a.998 CF₃ Cl H H F Cl —(CH₂)₅— I.a.999 F F I F F Cl —(CH₂)₅— I.a.1000 F H H H F C₂H₅ CH₃ H I.a.1001 Cl H H H F C₂H₅ CH₃ H I.a.1002 Br H H H F C₂H₅ CH₃ H I.a.1003 CN H H H F C₂H₅ CH₃ H I.a.1004 CH₃ H H H F C₂H₅ CH₃ H I.a.1005 F H H F F C₂H₅ CH₃ H I.a.1006 Cl H H F F C₂H₅ CH₃ H I.a.1007 F H H Cl F C₂H₅ CH₃ H I.a.1008 Cl H H F F C₂H₅ CH₃ H I.a.1009 CN H H F F C₂H₅ CH₃ H I.a.1010 F H H CN F C₂H₅ CH₃ H I.a.1011 CN H H F F C₂H₅ CH₃ H I.a.1012 F H F H F C₂H₅ CH₃ H I.a.1013 Cl H F H F C₂H₅ CH₃ H I.a.1014 CN H F H F C₂H₅ CH₃ H I.a.1015 F F F H F C₂H₅ CH₃ H I.a.1016 Cl F F H F C₂H₅ CH₃ H I.a.1017 F Cl F H F C₂H₅ CH₃ H I.a.1018 Cl F F H F C₂H₅ CH₃ H I.a.1019 CN F F H F C₂H₅ CH₃ H I.a.1020 F CN F H F C₂H₅ CH₃ H I.a.1021 CN F F H F C₂H₅ CH₃ H I.a.1022 F F H F F C₂H₅ CH₃ H I.a.1023 Cl F H F F C₂H₅ CH₃ H I.a.1024 F Cl H F F C₂H₅ CH₃ H I.a.1025 CN F H F F C₂H₅ CH₃ H I.a.1026 F CN H F F C₂H₅ CH₃ H I.a.1027 F F F F F C₂H₅ CH₃ H I.a.1028 Cl F F F F C₂H₅ CH₃ H I.a.1029 F Cl F F F C₂H₅ CH₃ H I.a.1030 CN F F F F C₂H₅ CH₃ H I.a.1031 F CN F F F C₂H₅ CH₃ H I.a.1032 H F F F F C₂H₅ CH₃ H I.a.1033 F F Br F F C₂H₅ CH₃ H I.a.1034 F F C≡CH F F C₂H₅ CH₃ H I.a.1035 CF₃ Cl H H F C₂H₅ CH₃ H I.a.1036 F F I F F C₂H₅ CH₃ H I.a.1037 F H H H F C₂H₅ C₂H₅ H I.a.1038 Cl H H H F C₂H₅ C₂H₅ H I.a.1039 Br H H H F C₂H₅ C₂H₅ H I.a.1040 CN H H H F C₂H₅ C₂H₅ H I.a.1041 CH₃ H H H F C₂H₅ C₂H₅ H I.a.1042 F H H F F C₂H₅ C₂H₅ H I.a.1043 Cl H H F F C₂H₅ C₂H₅ H I.a.1044 F H H Cl F C₂H₅ C₂H₅ H I.a.1045 Cl H H F F C₂H₅ C₂H₅ H I.a.1046 CN H H F F C₂H₅ C₂H₅ H I.a.1047 F H H CN F C₂H₅ C₂H₅ H I.a.1048 CN H H F F C₂H₅ C₂H₅ H I.a.1049 F H F H F C₂H₅ C₂H₅ H I.a.1050 Cl H F H F C₂H₅ C₂H₅ H I.a.1051 CN H F H F C₂H₅ C₂H₅ H I.a.1052 F F F H F C₂H₅ C₂H₅ H I.a.1053 Cl F F H F C₂H₅ C₂H₅ H I.a.1054 F Cl F H F C₂H₅ C₂H₅ H I.a.1055 Cl F F H F C₂H₅ C₂H₅ H I.a.1056 CN F F H F C₂H₅ C₂H₅ H I.a.1057 F CN F H F C₂H₅ C₂H₅ H I.a.1058 CN F F H F C₂H₅ C₂H₅ H I.a.1059 F F H F F C₂H₅ C₂H₅ H I.a.1060 Cl F H F F C₂H₅ C₂H₅ H I.a.1061 F Cl H F F C₂H₅ C₂H₅ H I.a.1062 CN F H F F C₂H₅ C₂H₅ H I.a.1063 F CN H F F C₂H₅ C₂H₅ H I.a.1064 F F F F F C₂H₅ C₂H₅ H I.a.1065 Cl F F F F C₂H₅ C₂H₅ H I.a.1066 F Cl F F F C₂H₅ C₂H₅ H I.a.1067 CN F F F F C₂H₅ C₂H₅ H I.a.1068 F CN F F F C₂H₅ C₂H₅ H I.a.1069 H F F F F C₂H₅ C₂H₅ H I.a.1070 F F Br F F C₂H₅ C₂H₅ H I.a.1071 F F C≡CH F F C₂H₅ C₂H₅ H I.a.1072 CF₃ Cl H H F C₂H₅ C₂H₅ H I.a.1073 F F I F F C₂H₅ C₂H₅ H I.a.1074 F H H H F C₂H₅ C₂H₅ CH₃ I.a.1075 Cl H H H F C₂H₅ C₂H₅ CH₃ I.a.1076 Br H H H F C₂H₅ C₂H₅ CH₃ I.a.1077 CN H H H F C₂H₅ C₂H₅ CH₃ I.a.1078 CH₃ H H H F C₂H₅ C₂H₅ CH₃ I.a.1079 F H H F F C₂H₅ C₂H₅ CH₃ I.a.1080 Cl H H F F C₂H₅ C₂H₅ CH₃ I.a.1081 F H H Cl F C₂H₅ C₂H₅ CH₃ I.a.1082 Cl H H F F C₂H₅ C₂H₅ CH₃ I.a.1083 CN H H F F C₂H₅ C₂H₅ CH₃ I.a.1084 F H H CN F C₂H₅ C₂H₅ CH₃ I.a.1085 CN H H F F C₂H₅ C₂H₅ CH₃ I.a.1086 F H F H F C₂H₅ C₂H₅ CH₃ I.a.1087 Cl H F H F C₂H₅ C₂H₅ CH₃ I.a.1088 CN H F H F C₂H₅ C₂H₅ CH₃ I.a.1089 F F F H F C₂H₅ C₂H₅ CH₃ I.a.1090 Cl F F H F C₂H₅ C₂H₅ CH₃ I.a.1091 F Cl F H F C₂H₅ C₂H₅ CH₃ I.a.1092 Cl F F H F C₂H₅ C₂H₅ CH₃ I.a.1093 CN F F H F C₂H₅ C₂H₅ CH₃ I.a.1094 F CN F H F C₂H₅ C₂H₅ CH₃ I.a.1095 CN F F H F C₂H₅ C₂H₅ CH₃ I.a.1096 F F H F F C₂H₅ C₂H₅ CH₃ I.a.1097 Cl F H F F C₂H₅ C₂H₅ CH₃ I.a.1098 F Cl H F F C₂H₅ C₂H₅ CH₃ I.a.1099 CN F H F F C₂H₅ C₂H₅ CH₃ I.a.1100 F CN H F F C₂H₅ C₂H₅ CH₃ I.a.1101 F F F F F C₂H₅ C₂H₅ CH₃ I.a.1102 Cl F F F F C₂H₅ C₂H₅ CH₃ I.a.1103 F Cl F F F C₂H₅ C₂H₅ CH₃ I.a.1104 CN F F F F C₂H₅ C₂H₅ CH₃ I.a.1105 F CN F F F C₂H₅ C₂H₅ CH₃ I.a.1106 H F F F F C₂H₅ C₂H₅ CH₃ I.a.1107 F F Br F F C₂H₅ C₂H₅ CH₃ I.a.1108 F F C≡CH F F C₂H₅ C₂H₅ CH₃ I.a.1109 CF₃ Cl H H F C₂H₅ C₂H₅ CH₃ I.a.1110 F F I F F C₂H₅ C₂H₅ CH₃ I.a.1111 F H H H F C₂H₅ CH₃ CH₃ I.a.1112 Cl H H H F C₂H₅ CH₃ CH₃ I.a.1113 Br H H H F C₂H₅ CH₃ CH₃ I.a.1114 CN H H H F C₂H₅ CH₃ CH₃ I.a.1115 CH₃ H H H F C₂H₅ CH₃ CH₃ I.a.1116 F H H F F C₂H₅ CH₃ CH₃ I.a.1117 Cl H H F F C₂H₅ CH₃ CH₃ I.a.1118 F H H Cl F C₂H₅ CH₃ CH₃ I.a.1119 Cl H H F F C₂H₅ CH₃ CH₃ I.a.1120 CN H H F F C₂H₅ CH₃ CH₃ I.a.1121 F H H CN F C₂H₅ CH₃ CH₃ I.a.1122 CN H H F F C₂H₅ CH₃ CH₃ I.a.1123 F H F H F C₂H₅ CH₃ CH₃ I.a.1124 Cl H F H F C₂H₅ CH₃ CH₃ I.a.1125 CN H F H F C₂H₅ CH₃ CH₃ I.a.1126 F F F H F C₂H₅ CH₃ CH₃ I.a.1127 Cl F F H F C₂H₅ CH₃ CH₃ I.a.1128 F Cl F H F C₂H₅ CH₃ CH₃ I.a.1129 Cl F F H F C₂H₅ CH₃ CH₃ I.a.1130 CN F F H F C₂H₅ CH₃ CH₃ I.a.1131 F CN F H F C₂H₅ CH₃ CH₃ I.a.1132 CN F F H F C₂H₅ CH₃ CH₃ I.a.1133 F F H F F C₂H₅ CH₃ CH₃ I.a.1134 Cl F H F F C₂H₅ CH₃ CH₃ I.a.1135 F Cl H F F C₂H₅ CH₃ CH₃ I.a.1136 CN F H F F C₂H₅ CH₃ CH₃ I.a.1137 F CN H F F C₂H₅ CH₃ CH₃ I.a.1138 F F F F F C₂H₅ CH₃ CH₃ I.a.1139 Cl F F F F C₂H₅ CH₃ CH₃ I.a.1140 F Cl F F F C₂H₅ CH₃ CH₃ I.a.1141 CN F F F F C₂H₅ CH₃ CH₃ I.a.1142 F CN F F F C₂H₅ CH₃ CH₃ I.a.1143 H F F F F C₂H₅ CH₃ CH₃ I.a.1144 F F Br F F C₂H₅ CH₃ CH₃ I.a.1145 F F C≡CH F F C₂H₅ CH₃ CH₃ I.a.1146 CF₃ Cl H H F C₂H₅ CH₃ CH₃ I.a.1147 F F I F F C₂H₅ CH₃ CH₃ I.a.1148 F H H H F Cl CH₃ H I.a.1149 Cl H H H F Cl CH₃ H I.a.1150 Br H H H F Cl CH₃ H I.a.1151 CN H H H F Cl CH₃ H I.a.1152 CH₃ H H H F Cl CH₃ H I.a.1153 F H H F F Cl CH₃ H I.a.1154 Cl H H F F Cl CH₃ H I.a.1155 F H H Cl F Cl CH₃ H I.a.1156 Cl H H F F Cl CH₃ H I.a.1157 CN H H F F Cl CH₃ H I.a.1158 F H H CN F Cl CH₃ H I.a.1159 CN H H F F Cl CH₃ H I.a.1160 F H F H F Cl CH₃ H I.a.1161 Cl H F H F Cl CH₃ H I.a.1162 CN H F H F Cl CH₃ H I.a.1163 F F F H F Cl CH₃ H I.a.1164 Cl F F H F Cl CH₃ H I.a.1165 F Cl F H F Cl CH₃ H I.a.1166 Cl F F H F Cl CH₃ H I.a.1167 CN F F H F Cl CH₃ H I.a.1168 F CN F H F Cl CH₃ H I.a.1169 CN F F H F Cl CH₃ H I.a.1170 F F H F F Cl CH₃ H I.a.1171 Cl F H F F Cl CH₃ H I.a.1172 F Cl H F F Cl CH₃ H I.a.1173 CN F H F F Cl CH₃ H I.a.1174 F CN H F F Cl CH₃ H I.a.1175 F F F F F Cl CH₃ H I.a.1176 Cl F F F F Cl CH₃ H I.a.1177 F Cl F F F Cl CH₃ H I.a.1178 CN F F F F Cl CH₃ H I.a.1179 F CN F F F Cl CH₃ H I.a.1180 H F F F F Cl CH₃ H I.a.1181 F F Br F F Cl CH₃ H I.a.1182 F F C≡CH F F Cl CH₃ H I.a.1183 CF₃ Cl H H F Cl CH₃ H I.a.1184 F F I F F Cl CH₃ H I.a.1185 F H H H F CH₂Cl Cl CH₃ I.a.1186 Cl H H H F CH₂Cl Cl CH₃ I.a.1187 Br H H H F CH₂Cl Cl CH₃ I.a.1188 CN H H H F CH₂Cl Cl CH₃ I.a.1189 CH₃ H H H F CH₂Cl Cl CH₃ I.a.1190 F H H F F CH₂Cl Cl CH₃ I.a.1191 Cl H H F F CH₂Cl Cl CH₃ I.a.1192 F H H Cl F CH₂Cl Cl CH₃ I.a.1193 Cl H H F F CH₂Cl Cl CH₃ I.a.1194 CN H H F F CH₂Cl Cl CH₃ I.a.1195 F H H CN F CH₂Cl Cl CH₃ I.a.1196 CN H H F F CH₂Cl Cl CH₃ I.a.1197 F H F H F CH₂Cl Cl CH₃ I.a.1198 Cl H F H F CH₂Cl Cl CH₃ I.a.1199 CN H F H F CH₂Cl Cl CH₃ I.a.1200 F F F H F CH₂Cl Cl CH₃ I.a.1201 Cl F F H F CH₂Cl Cl CH₃ I.a.1202 F Cl F H F CH₂Cl Cl CH₃ I.a.1203 Cl F F H F CH₂Cl Cl CH₃ I.a.1204 CN F F H F CH₂Cl Cl CH₃ I.a.1205 F CN F H F CH₂Cl Cl CH₃ I.a.1206 CN F F H F CH₂Cl Cl CH₃ I.a.1207 F F H F F CH₂Cl Cl CH₃ I.a.1208 Cl F H F F CH₂Cl Cl CH₃ I.a.1209 F Cl H F F CH₂Cl Cl CH₃ I.a.1210 CN F H F F CH₂Cl Cl CH₃ I.a.1211 F CN H F F CH₂Cl Cl CH₃ I.a.1212 F F F F F CH₂Cl Cl CH₃ I.a.1213 Cl F F F F CH₂Cl Cl CH₃ I.a.1214 F Cl F F F CH₂Cl Cl CH₃ I.a.1215 CN F F F F CH₂Cl Cl CH₃ I.a.1216 F CN F F F CH₂Cl Cl CH₃ I.a.1217 H F F F F CH₂Cl Cl CH₃ I.a.1218 F F Br F F CH₂Cl Cl CH₃ I.a.1219 F F C≡CH F F CH₂Cl Cl CH₃ I.a.1220 CF₃ Cl H H F CH₂Cl Cl CH₃ I.a.1221 F F I F F CH₂Cl Cl CH₃ I.a.1222 F H H H F CN CH₃ CH₃ I.a.1223 Cl H H H F CN CH₃ CH₃ I.a.1224 Br H H H F CN CH₃ CH₃ I.a.1225 CN H H H F CN CH₃ CH₃ I.a.1226 CH₃ H H H F CN CH₃ CH₃ I.a.1227 F H H F F CN CH₃ CH₃ I.a.1228 Cl H H F F CN CH₃ CH₃ I.a.1229 F H H Cl F CN CH₃ CH₃ I.a.1230 Cl H H F F CN CH₃ CH₃ I.a.1231 CN H H F F CN CH₃ CH₃ I.a.1232 F H H CN F CN CH₃ CH₃ I.a.1233 CN H H F F CN CH₃ CH₃ I.a.1234 F H F H F CN CH₃ CH₃ I.a.1235 Cl H F H F CN CH₃ CH₃ I.a.1236 CN H F H F CN CH₃ CH₃ I.a.1237 F F F H F CN CH₃ CH₃ I.a.1238 Cl F F H F CN CH₃ CH₃ I.a.1239 F Cl F H F CN CH₃ CH₃ I.a.1240 Cl F F H F CN CH₃ CH₃ I.a.1241 CN F F H F CN CH₃ CH₃ I.a.1242 F CN F H F CN CH₃ CH₃ I.a.1243 CN F F H F CN CH₃ CH₃ I.a.1244 F F H F F CN CH₃ CH₃ I.a.1245 Cl F H F F CN CH₃ CH₃ I.a.1246 F Cl H F F CN CH₃ CH₃ I.a.1247 CN F H F F CN CH₃ CH₃ I.a.1248 F CN H F F CN CH₃ CH₃ I.a.1249 F F F F F CN CH₃ CH₃ I.a.1250 Cl F F F F CN CH₃ CH₃ I.a.1251 F Cl F F F CN CH₃ CH₃ I.a.1252 CN F F F F CN CH₃ CH₃ I.a.1253 F CN F F F CN CH₃ CH₃ I.a.1254 H F F F F CN CH₃ CH₃ I.a.1255 F F Br F F CN CH₃ CH₃ I.a.1256 F F C≡CH F F CN CH₃ CH₃ I.a.1257 CF₃ Cl H H F CN CH₃ CH₃ I.a.1258 F F I F F CN CH₃ CH₃ I.a.1259 F H H H F OCH₃ H H I.a.1260 Cl H H H F OCH₃ H H I.a.1261 Br H H H F OCH₃ H H I.a.1262 CN H H H F OCH₃ H H I.a.1263 CH₃ H H H F OCH₃ H H I.a.1264 F H H F F OCH₃ H H I.a.1265 Cl H H F F OCH₃ H H I.a.1266 F H H Cl F OCH₃ H H I.a.1267 Cl H H F F OCH₃ H H I.a.1268 CN H H F F OCH₃ H H I.a.1269 F H H CN F OCH₃ H H I.a.1270 CN H H F F OCH₃ H H I.a.1271 F H F H F OCH₃ H H I.a.1272 Cl H F H F OCH₃ H H I.a.1273 CN H F H F OCH₃ H H I.a.1274 F F F H F OCH₃ H H I.a.1275 Cl F F H F OCH₃ H H I.a.1276 F Cl F H F OCH₃ H H I.a.1277 Cl F F H F OCH₃ H H I.a.1278 CN F F H F OCH₃ H H I.a.1279 F CN F H F OCH₃ H H I.a.1280 CN F F H F OCH₃ H H I.a.1281 F F H F F OCH₃ H H I.a.1282 Cl F H F F OCH₃ H H I.a.1283 F Cl H F F OCH₃ H H I.a.1284 CN F H F F OCH₃ H H I.a.1285 F CN H F F OCH₃ H H I.a.1286 F F F F F OCH₃ H H I.a.1287 Cl F F F F OCH₃ H H I.a.1288 F Cl F F F OCH₃ H H I.a.1289 CN F F F F OCH₃ H H I.a.1290 F CN F F F OCH₃ H H I.a.1291 H F F F F OCH₃ H H I.a.1292 F F Br F F OCH₃ H H I.a.1293 F F C≡CH F F OCH₃ H H I.a.1294 CF₃ Cl H H F OCH₃ H H I.a.1295 F F I F F OCH₃ H H I.a.1296 F H H H F OCH₃ CH₃ H I.a.1297 Cl H H H F OCH₃ CH₃ H I.a.1298 Br H H H F OCH₃ CH₃ H I.a.1299 CN H H H F OCH₃ CH₃ H I.a.1300 CH₃ H H H F OCH₃ CH₃ H I.a.1301 F H H F F OCH₃ CH₃ H I.a.1302 Cl H H F F OCH₃ CH₃ H I.a.1303 F H H Cl F OCH₃ CH₃ H I.a.1304 Cl H H F F OCH₃ CH₃ H I.a.1305 CN H H F F OCH₃ CH₃ H I.a.1306 F H H CN F OCH₃ CH₃ H I.a.1307 CN H H F F OCH₃ CH₃ H I.a.1308 F H F H F OCH₃ CH₃ H I.a.1309 Cl H F H F OCH₃ CH₃ H I.a.1310 CN H F H F OCH₃ CH₃ H I.a.1311 F F F H F OCH₃ CH₃ H I.a.1312 Cl F F H F OCH₃ CH₃ H I.a.1313 F Cl F H F OCH₃ CH₃ H I.a.1314 Cl F F H F OCH₃ CH₃ H I.a.1315 CN F F H F OCH₃ CH₃ H I.a.1316 F CN F H F OCH₃ CH₃ H I.a.1317 CN F F H F OCH₃ CH₃ H I.a.1318 F F H F F OCH₃ CH₃ H I.a.1319 Cl F H F F OCH₃ CH₃ H I.a.1320 F Cl H F F OCH₃ CH₃ H I.a.1321 CN F H F F OCH₃ CH₃ H I.a.1322 F CN H F F OCH₃ CH₃ H I.a.1323 F F F F F OCH₃ CH₃ H I.a.1324 Cl F F F F OCH₃ CH₃ H I.a.1325 F Cl F F F OCH₃ CH₃ H I.a.1326 CN F F F F OCH₃ CH₃ H I.a.1327 F CN F F F OCH₃ CH₃ H I.a.1328 H F F F F OCH₃ CH₃ H I.a.1329 F F Br F F OCH₃ CH₃ H I.a.1330 F F C≡CH F F OCH₃ CH₃ H I.a.1331 CF₃ Cl H H F OCH₃ CH₃ H I.a.1332 F F I F F OCH₃ CH₃ H I.a.1333 F H H H F OCH₃ CH₃ CH₃ I.a.1334 Cl H H H F OCH₃ CH₃ CH₃ I.a.1335 Br H H H F OCH₃ CH₃ CH₃ I.a.1336 CN H H H F OCH₃ CH₃ CH₃ I.a.1337 CH₃ H H H F OCH₃ CH₃ CH₃ I.a.1338 F H H F F OCH₃ CH₃ CH₃ I.a.1339 Cl H H F F OCH₃ CH₃ CH₃ I.a.1340 F H H Cl F OCH₃ CH₃ CH₃ I.a.1341 Cl H H F F OCH₃ CH₃ CH₃ I.a.1342 CN H H F F OCH₃ CH₃ CH₃ I.a.1343 F H H CN F OCH₃ CH₃ CH₃ I.a.1344 CN H H F F OCH₃ CH₃ CH₃ I.a.1345 F H F H F OCH₃ CH₃ CH₃ I.a.1346 Cl H F H F OCH₃ CH₃ CH₃ I.a.1347 CN H F H F OCH₃ CH₃ CH₃ I.a.1348 F F F H F OCH₃ CH₃ CH₃ I.a.1349 Cl F F H F OCH₃ CH₃ CH₃ I.a.1350 F Cl F H F OCH₃ CH₃ CH₃ I.a.1351 Cl F F H F OCH₃ CH₃ CH₃ I.a.1352 CN F F H F OCH₃ CH₃ CH₃ I.a.1353 F CN F H F OCH₃ CH₃ CH₃ I.a.1354 CN F F H F OCH₃ CH₃ CH₃ I.a.1355 F F H F F OCH₃ CH₃ CH₃ I.a.1356 Cl F H F F OCH₃ CH₃ CH₃ I.a.1357 F Cl H F F OCH₃ CH₃ CH₃ I.a.1358 CN F H F F OCH₃ CH₃ CH₃ I.a.1359 F CN H F F OCH₃ CH₃ CH₃ I.a.1360 F F F F F OCH₃ CH₃ CH₃ I.a.1361 Cl F F F F OCH₃ CH₃ CH₃ I.a.1362 F Cl F F F OCH₃ CH₃ CH₃ I.a.1363 CN F F F F OCH₃ CH₃ CH₃ I.a.1364 F CN F F F OCH₃ CH₃ CH₃ I.a.1365 H F F F F OCH₃ CH₃ CH₃ I.a.1366 F F Br F F OCH₃ CH₃ CH₃ I.a.1367 F F C≡CH F F OCH₃ CH₃ CH₃ I.a.1368 CF₃ Cl H H F OCH₃ CH₃ CH₃ I.a.1369 F F I F F OCH₃ CH₃ CH₃ I.a.1370 F H H H F H —O(CH₂)₃— I.a.1371 Cl H H H F H —O(CH₂)₃— I.a.1372 Br H H H F H —O(CH₂)₃— I.a.1373 CN H H H F H —O(CH₂)₃— I.a.1374 CH₃ H H H F H —O(CH₂)₃— I.a.1375 F H H F F H —O(CH₂)₃— I.a.1376 Cl H H F F H —O(CH₂)₃— I.a.1377 F H H Cl F H —O(CH₂)₃— I.a.1378 Cl H H F F H —O(CH₂)₃— I.a.1379 CN H H F F H —O(CH₂)₃— I.a.1380 F H H CN F H —O(CH₂)₃— I.a.1381 CN H H F F H —O(CH₂)₃— I.a.1382 F H F H F H —O(CH₂)₃— I.a.1383 Cl H F H F H —O(CH₂)₃— I.a.1384 CN H F H F H —O(CH₂)₃— I.a.1385 F F F H F H —O(CH₂)₃— I.a.1386 Cl F F H F H —O(CH₂)₃— I.a.1387 F Cl F H F H —O(CH₂)₃— I.a.1388 Cl F F H F H —O(CH₂)₃— I.a.1389 CN F F H F H —O(CH₂)₃— I.a.1390 F CN F H F H —O(CH₂)₃— I.a.1391 CN F F H F H —O(CH₂)₃— I.a.1392 F F H F F H —O(CH₂)₃— I.a.1393 Cl F H F F H —O(CH₂)₃— I.a.1394 F Cl H F F H —O(CH₂)₃— I.a.1395 CN F H F F H —O(CH₂)₃— I.a.1396 F CN H F F H —O(CH₂)₃— I.a.1397 F F F F F H —O(CH₂)₃— I.a.1398 Cl F F F F H —O(CH₂)₃— I.a.1399 F Cl F F F H —O(CH₂)₃— I.a.1400 CN F F F F H —O(CH₂)₃— I.a.1401 F CN F F F H —O(CH₂)₃— I.a.1402 H F F F F H —O(CH₂)₃— I.a.1403 F F Br F F H —O(CH₂)₃— I.a.1404 F F C≡CH F F H —O(CH₂)₃— I.a.1405 CF₃ Cl H H F H —O(CH₂)₃— I.a.1406 F F I F F H —O(CH₂)₃—

In another preferred embodiment, the azines useful for the present invention are azines of formula (I)

wherein

-   A is heteroaryl, which is substituted by 1, 2, 3, 4, 5 or 6     substituents R^(A) selected from the group consisting of halogen,     OH, CN, amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,     C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,     (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,     C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,     (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,     (C₁-C₆-alkoxy)-carbonyl, (C₁-C₆-alkyl)-carbonyloxy,     C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy, (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and     cycloaliphatic parts of the 22 aforementioned radicals are     unsubstituted, partly or completely halogenated and where the     cycloaliphatic parts of the last 4 mentioned radicals may carry 1,     2, 3, 4, 5 or 6 methyl groups; -   R¹ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,     C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,     (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,     (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,     (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and     (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic parts     of the 14 aforementioned radicals are unsubstituted, partly or     completely halogenated, phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl,     phenylaminosulfonyl, phenylcarbonyl and phenoxycarbonyl,     -   wherein phenyl in the last 6 mentioned radicals are         unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or         different substituents selected from the group consisting of         halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and         C₁-C₆-haloalkoxy; -   R² is H, halogen, OH, CN, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy,     C₂-C₆-alkynyloxy, C₃-C₆-cycloalkoxy or     (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and     cycloaliphatic parts of the 9 aforementioned radicals are     unsubstituted, partly or completely halogenated,     -   phenyl, phenyl-C₁-C₆-alkyl,     -   wherein phenyl in the last 2 mentioned radicals are         unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or         different substituents selected from the group consisting of         halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and         C₁-C₆-haloalkoxy; -   R³ is selected from the group consisting of H, halogen, CN,     C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy; -   R⁴ is selected from the group consisting of H, halogen, CN,     C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl, C₃-C₆-cycloalkyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₃-C₆-cycloalkenyl and     C₁-C₆-alkoxy-C₁-C₆-alkyl, where the aliphatic and cycloaliphatic     parts of the 7 aforementioned radicals are unsubstituted, partly or     completely halogenated; or -   R³ and R⁴ together with the carbon atom to which they are attached     form a moiety selected from the group consisting of carbonyl,     C₂-C₆-alkenyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl and three- to     six-membered heterocyclyl,     -   wherein the C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl, or three- to         six-membered heterocyclyl is unsubstituted or substituted by one         to three substituents selected from halogen, CN, C₁-C₆-alkyl and         C₁-C₆-alkoxy; and -   R⁵ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,     C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,     (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,     (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,     (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and     (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic parts     of the 14 aforementioned radicals are unsubstituted, partly or     completely halogenated, phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl,     phenylaminosulfonyl, phenylcarbonyl and phenoxycarbonyl,     -   wherein phenyl in the last 6 mentioned radicals are         unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or         different substituents selected from the group consisting of         halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and         C₁-C₆-haloalkoxy;         and wherein the variables A, R¹, R², R³, R⁴ and R⁵ are in         particular: -   A is heteroaryl, which is substituted by 1, 2, 3, 4, 5 or 6     substituents R^(A) selected from the group consisting of halogen,     OH, CN, amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,     C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,     (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,     C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,     (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,     (C₁-C₆-alkoxy)-carbonyl, (C₁-C₆-alkyl)-carbonyloxy,     C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy, (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and     cycloaliphatic parts of the 22 aforementioned radicals are     unsubstituted, partly or completely halogenated and where the     cycloaliphatic parts of the last 4 mentioned radicals may carry 1,     2, 3, 4, 5 or 6 methyl groups; -   R¹ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,     C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,     (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,     (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,     (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and     (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic parts     of the 14 aforementioned radicals are unsubstituted, partly or     completely halogenated,     -   phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl, phenylaminosulfonyl,         phenylcarbonyl and phenoxycarbonyl,     -   wherein phenyl in the last 6 mentioned radicals are         unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or         different substituents selected from the group consisting of         halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and         C₁-C₆-haloalkoxy; -   R² is H, halogen, OH, CN, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy,     C₂-C₆-alkynyloxy, C₃-C₆-cycloalkoxy or     (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and     cycloaliphatic parts of the 9 aforementioned radicals are     unsubstituted, partly or completely halogenated or phenyl, wherein     phenyl is unsubstituted or substituted by 1, 2, 3, 4 or 5 identical     or different substituents selected from the group consisting of     halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and     C₁-C₆-haloalkoxy;     -   R³ is selected from the group consisting of H, halogen, CN,         C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy;     -   R⁴ is selected from the group consisting of H, halogen, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl, C₃-C₆-cycloalkyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₃-C₆-cycloalkenyl and         C₁-C₆-alkoxy-C₁-C₆-alkyl, where the aliphatic and cycloaliphatic         parts of the 7 aforementioned radicals are unsubstituted, partly         or completely halogenated; or     -   R³ and R⁴ together with the carbon atom to which they are         attached form a moiety selected from the group consisting of         carbonyl, C₂-C₆-alkenyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl         and three- to six-membered heterocyclyl,     -   wherein the C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl, or three- to         six-membered heterocyclyl is unsubstituted or substituted by one         to three substituents selected from halogen, CN, C₁-C₆-alkyl and         C₁-C₆-alkoxy; and     -   R⁵ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,         (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,         (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and         (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic         parts of the 14 aforementioned radicals are unsubstituted,         partly or completely halogenated,     -   phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl, phenylaminosulfonyl,         phenylcarbonyl and phenoxycarbonyl,     -   wherein phenyl in the last 6 mentioned radicals are         unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or         different substituents selected from the group consisting of         halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and         C₁-C₆-haloalkoxy;         and wherein the variables A, R¹, R², R³, R⁴ and R⁵ are in more         particular: -   A is heteroaryl, which is substituted by 1, 2, 3, 4, 5 or 6     substituents R^(A) selected from the group consisting of halogen,     OH, CN, amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,     C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,     (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,     C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,     (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)-carbonyl,     (C₁-C₆-alkoxy)-carbonyl, (C₁-C₆-alkyl)-carbonyloxy,     C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy, (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and     cycloaliphatic parts of the 22 aforementioned radicals are     unsubstituted, partly or completely halogenated and where the     cycloaliphatic parts of the last 4 mentioned radicals may carry 1,     2, 3, 4, 5 or 6 methyl groups; -   R¹ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,     C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,     (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,     (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,     (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and     (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic parts     of the 14 aforementioned radicals are unsubstituted, partly or     completely halogenated, -   phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl, phenylaminosulfonyl,     phenylcarbonyl and phenoxycarbonyl, -   wherein phenyl in the last 6 mentioned radicals are unsubstituted or     substituted by 1, 2, 3, 4 or 5 identical or different substituents     selected from the group consisting of halogen, CN, NO₂, C₁-C₆-alkyl,     C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy; -   R² is H, halogen, OH, CN, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy,     C₂-C₆-alkynyloxy, C₃-C₆-cycloalkoxy or     (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and     cycloaliphatic parts of the 9 aforementioned radicals are     unsubstituted, partly or completely halogenated, -   R³ is selected from the group consisting of H, halogen, CN,     C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy; -   R⁴ is selected from the group consisting of H, halogen, CN,     C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl, C₃-C₆-cycloalkyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₃-C₆-cycloalkenyl and     C₁-C₆-alkoxy-C₁-C₆-alkyl, where the aliphatic and cycloaliphatic     parts of the 7 aforementioned radicals are unsubstituted, partly or     completely halogenated; or -   R³ and R⁴ together with the carbon atom to which they are attached     form a moiety selected from the group consisting of carbonyl,     C₂-C₆-alkenyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl and three- to     six-membered heterocyclyl, -   wherein the C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl, or three- to     six-membered heterocyclyl is unsubstituted or substituted by one to     three substituents selected from halogen, CN, C₁-C₆-alkyl and     C₁-C₆-alkoxy; and -   R⁵ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,     C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,     (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,     (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,     (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and     (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic parts     of the 14 aforementioned radicals are unsubstituted, partly or     completely halogenated, -   phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl, phenylaminosulfonyl,     phenylcarbonyl and phenoxycarbonyl, -   wherein phenyl in the last 6 mentioned radicals are unsubstituted or     substituted by 1, 2, 3, 4 or 5 identical or different substituents     selected from the group consisting of halogen, CN, NO₂, C₁-C₆-alkyl,     C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy;     and wherein the variables A, R¹, R², R³, R⁴ and R⁵ are preferred: -   A is heteroaryl, which is substituted by one to six substituents     selected from the group consisting of halogen, CN, NO₂, C₁-C₆-alkyl,     C₁-C₆-haloalkyl, OH, C₁-C₆-alkoxy, C₁-C₆-alkylthio,     (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl, amino,     (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino, (C₁-C₆-alkyl)carbonyl,     (C₁-C₆-alkoxy)carbonyl; -   R¹ is H, CN, C₁-C₆-alkyl, C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy,     (C₁-C₆-alkyl)carbonyl, (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl     or phenylsulfonyl, -   wherein the phenyl is unsubstituted or substituted by one to five     substituents selected from the group consisting of halogen, CN, NO₂,     C₁-C₆-alkyl, C₁-C₆-haloalkyl and C₁-C₆-alkoxy; -   R² is H, halogen, OH, CN, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl,     (C₁-C₆-alkoxy)-C₁-C₆-alkyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl,     (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy,     C₂-C₆-alkynyloxy, C₃-C₆-cycloalkoxy or     (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and     cycloaliphatic parts of the 9 aforementioned radicals are     unsubstituted, partly or completely halogenated -   R³ is H, halogen, CN, C₁-C₆-alkyl, C₁-C₆-haloalkyl or C₁-C₆-alkoxy; -   R⁴ is H, halogen, CN, C₁-C₆-alkyl or C₁-C₆-haloalkyl; or -   R³ and R⁴ together with the carbon atom to which they are attached     form a moiety selected from the group consisting of carbonyl,     C₂-C₆-alkenyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl and three- to     six-membered heterocyclyl, -   wherein the C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl, or three- to     six-membered heterocyclyl is unsubstituted or substituted by one to     three substituents selected from halogen, CN, C₁-C₆-alkyl and     C₁-C₆-alkoxy; and -   R⁵ is H, CN, C₁-C₆-alkyl, C₁-C₆-alkoxy-C₁-C₆-alkyl, C₁-C₆-alkoxy,     (C₁-C₆-alkyl)carbonyl, (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl     or phenylsulfonyl, -   wherein the phenyl is unsubstituted or substituted by one to five     substituents selected from the group consisting of halogen, CN, NO₂,     C₁-C₆-alkyl, C₁-C₆-haloalkyl and C₁-C₆-alkoxy;     including their agriculturally acceptable salts or N-oxides.

In another preferred embodiment, the azines useful for the present invention comprise a diaminotriazine compound of formula (I)

-   -   wherein     -   p is 1 or 2;     -   q is 0, 1, 2 or 3 provided that p+q is 1, 2, 3 or 4;     -   Q is a chemical bond, O, S(O)_(m), CR^(q1)R^(q2), NR^(q3), C(O),         C(O)O,         -   CR^(q1)R^(q2)—O, S(O)_(m)NR^(q3) or C(O)NR^(q3),         -   wherein         -   m is 0, 1 or 2;         -   R^(q1), R^(q2) are hydrogen, halogen or C₁-C₄-alkyl;         -   R^(q3) is H, CN, C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkyl,             (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl,             (C₁-C₆-alkyl)sulfonyl, where the aliphatic parts of the 6             aforementioned radicals are unsubstituted, partly or             completely halogenated;     -   Ar is phenyl, which is unsubstituted or carries 1, 2, 3, 4 or 5         radicals R^(A) which are selected from the group consisting of         halogen, OH, CN, amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl,         C₂-C₆-alkynyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)-carbonyl,         (C₁-C₆-alkyl)-carbonyloxy, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy,         where the aliphatic and cycloaliphatic parts of the 22         aforementioned radicals are unsubstituted, partly or completely         halogenated and where the cycloaliphatic parts of the last 4         mentioned radicals may carry 1, 2, 3, 4, 5 or 6 methyl groups,         -   phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl,             phenylaminosulfonyl, phenylaminocarbonyl,             phenyl(C₁-C₆-alkyl)aminocarbonyl, phenylcarbonyl and             phenoxycarbonyl,         -   wherein phenyl in the last 8 mentioned radicals are             unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or             different substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy             and C₁-C₆-haloalkoxy,         -   it being possible that R^(A) are identical or different;     -   R^(a) is selected from the group consisting of hydrogen,         halogen, OH, CN, amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl,         C₂-C₆-alkynyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)-carbonyl,         (C₁-C₆-alkyl)-carbonyloxy, where the aliphatic and         cycloaliphatic parts of the 14 aforementioned radicals are         unsubstituted, partly or completely halogenated;     -   R^(b) is selected from the group consisting of halogen, OH, CN,         amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)-carbonyl,         (C₁-C₆-alkyl)-carbonyloxy, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy,         where the aliphatic and cycloaliphatic parts of the 22         aforementioned radicals are unsubstituted, partly or completely         halogenated and where the cycloaliphatic parts of the last 4         mentioned radicals may carry 1, 2, 3, 4, 5 or 6 methyl groups,         -   for q=2 or 3 it being possible that R^(b) are identical or             different;     -   R¹ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₃-C₆-cycloalkyl)-carbonyl,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl,         (C₁-C₆-alkyl)sulfonyl, (C₁-C₆-alkylamino)carbonyl,         di(C₁-C₆-alkyl)aminocarbonyl, (C₁-C₆-alkylamino)sulfonyl,         di(C₁-C₆-alkyl)aminosulfonyl and (C₁-C₆-alkoxy)sulfonyl, where         the aliphatic and cycloaliphatic parts of the 15 aforementioned         radicals are unsubstituted, partly or completely halogenated,         phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl, phenylaminosulfonyl,         phenylaminocarbonyl, phenyl(C₁-C₆-alkyl)aminocarbonyl,         phenylcarbonyl and phenoxycarbonyl,         -   wherein phenyl in the last 8 mentioned radicals are             unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or             different substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy             and C₁-C₆-haloalkoxy;     -   R² is selected from the group consisting of H, OH, S(O)₂NH₂, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₃-C₆-cycloalkyl)-carbonyl         C₁-C₆-alkoxy, (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)carbonyl,         (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,         (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and         (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic         parts of the 15 aforementioned radicals are unsubstituted,         partly or completely halogenated, phenyl, phenylsulfonyl,         phenylaminosulfonyl, phenylaminocarbonyl,         phenyl(C₁-C₆-alkyl)aminocarbonyl, phenyl-C₁-C₆ alkyl, phenoxy,         phenylcarbonyl and phenoxycarbonyl,         -   wherein phenyl in the last 9 mentioned radicals is             unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or             different substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy             and C₁-C₆-haloalkoxy;     -   X is a radical selected from the group consisting of         -   CR³R⁴R⁵,         -   phenyl, which is unsubstituted or carries 1, 2, 3, 4 or 5             radicals R^(Ar) which are identical or different;         -   NR^(3a)R^(3b),         -   OR^(3c) and         -   S(O)_(k)R^(3d) with k being 0, 1 or 2     -   wherein     -   R³ is selected from the group consisting of H, halogen, OH, CN,         C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkyl, C₃-C₆-cycloalkyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy,         C₂-C₆-alkynyloxy, C₃-C₆-cycloalkoxy,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and         cycloaliphatic parts of the 9 aforementioned radicals are         unsubstituted, partly or completely halogenated;     -   R⁴ is selected from the group consisting of H, halogen, CN,         C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy;     -   R⁵ is selected from the group consisting of halogen, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl, C₃-C₆-cycloalkyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₃-C₆-cycloalkenyl and         C₁-C₆-alkoxy-C₁-C₆-alkyl, where the aliphatic and cycloaliphatic         parts of the 7 aforementioned radicals are unsubstituted, partly         or completely halogenated;     -   R⁴ and R⁵ together with the carbon atom to which they are         attached form a moiety selected from the group consisting of         carbonyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl, three- to         six-membered saturated or partially unsaturated heterocyclyl,         and the moiety >C═CR^(x)R^(y), where R^(x) and R^(y) are         hydrogen, C₁-C₄-alkyl or C₁-C₄-haloalkyl;     -   R^(Ar) selected from the group consisting of halogen, OH, CN,         amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)-carbonyl,         (C₁-C₆-alkyl)-carbonyloxy, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy,         where the aliphatic and cycloaliphatic parts of the 22         aforementioned radicals are unsubstituted, partly or completely         halogenated and where the cycloaliphatic parts of the last 4         mentioned radicals may carry 1, 2, 3, 4, 5 or 6 methyl groups,     -   R^(3a), R^(3b), R^(3c) or R^(3d) are independently of one         another are selected from the group consisting of H, CN,         S(O)₂NH₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         C₃-C₆-cycloalkyl, (C₃-C₆-cycloalkyl)-C₁-C₆-alkyl,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,         (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,         (C₃-C₆-cycloalkyl)-carbonyl, (C₁-C₆-alkylamino)sulfonyl,         di(C₁-C₆-alkyl)aminosulfonyl and (C₁-C₆-alkoxy)sulfonyl, where         the aliphatic and cycloaliphatic parts of the 15 aforementioned         radicals are unsubstituted, partly or completely halogenated,         phenyl, phenylsulfonyl, phenyl-C₁-C₆ alkyl, phenylaminosulfonyl,         phenylcarbonyl and phenoxycarbonyl, wherein phenyl in the last 6         mentioned radicals is unsubstituted or substituted by 1, 2, 3, 4         or 5 identical or different substituents selected from the group         consisting of halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl,         C₁-C₆-alkoxy and C₁-C₆-haloalkoxy, or     -   R^(3a), R^(3b) together with the nitrogen atom, to which they         are bound, form an N-bound, mono- or bicyclic heterocyclic         radical, which may have 1, 2, 3 or 4 further heteroatoms which         are selected from N, O and S, which is unsubstituted or         substituted by one or more identical or different substituents         selected from the group consisting of halogen, CN, NO₂,         C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy,         -   one of R^(3a), R^(3b) may also be OH, C₁-C₆-alkoxy,             C₃-C₆-cycloalkoxy, (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy             C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,             (C₁-C₆-alkoxy)-C₁-C₆-alkoxy, where the aliphatic and             cycloaliphatic parts of the 7 aforementioned radicals are             unsubstituted, partly or completely halogenated,         -   or phenoxy, which is unsubstituted or substituted by 1, 2,             3, 4 or 5 identical or different substituents selected from             the group consisting of halogen, CN, NO₂, C₁-C₆-alkyl,             C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy;             including their agriculturally acceptable salts

In another preferred embodiment, the azines useful for the present invention comprises a diaminotriazine compound of the formula:

-   -   wherein     -   A is a fused saturated or unsaturated, 5- or 6-membered         carbocycle or a fused saturated or unsaturated, 5- or 6-membered         heterocycle having 1, 2 or 3 heteroatoms or heteroatom moieties,         selected from O, S, S(O)_(p), N or NR^(c) as ring members,         -   where the carbocycle and the heterocycle are unsubstituted             or carry 1, 2, 3 or 4 radicals R^(A);     -   p is 0, 1 or 2     -   q is 0, 1, 2 or 3;     -   R^(A) is selected from the group consisting of halogen, OH, CN,         amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)-carbonyl,         (C₁-C₆-alkyl)-carbonyloxy, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy,         where the aliphatic and cycloaliphatic parts of the 22         aforementioned radicals are unsubstituted, partly or completely         halogenated and where the cycloaliphatic parts of the last 4         mentioned radicals may carry 1, 2, 3, 4, 5 or 6 methyl groups,         -   it being possible that R^(A) are identical or different, it             being possible that two radicals R^(A) which are bound at             the same carbon atom may together be ═O or ═NR^(d);     -   R^(b) is selected from the group consisting of halogen, OH, CN,         amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)-carbonyl,         (C₁-C₆-alkyl)-carbonyloxy, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy,         where the aliphatic and cycloaliphatic parts of the 22         aforementioned radicals are unsubstituted, partly or completely         halogenated and where the cycloaliphatic parts of the last 4         mentioned radicals may carry 1, 2, 3, 4, 5 or 6 methyl groups,         -   for q=2 or 3 it being possible that R^(b) are identical or             different;     -   R^(c) is selected from the group consisting of H, OH, S(O)₂NH₂,         CN, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl, C₃-C₆-cycloalkyl,         C₃-C₆-cycloalkoxy, (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,         (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,         (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and         (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic         parts of the 16 aforementioned radicals are unsubstituted,         partly or completely halogenated,     -   R^(d) is selected from the group consisting of H, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy,         C₂-C₆-alkynyloxy, (C₁-C₆-alkoxy)-C₁-C₆-alkyl, where the         aliphatic and cycloaliphatic parts of the 8 aforementioned         radicals are unsubstituted, partly or completely halogenated;     -   R¹ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,         C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy, C₁-C₆-alkyl, C₂-C₆-alkenyl,         C₂-C₆-alkynyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,         (C₃-C₆-cycloalkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl,         (C₁-C₆-alkyl)sulfonyl, (C₁-C₆-alkylamino)carbonyl,         di(C₁-C₆-alkyl)aminocarbonyl, (C₁-C₆-alkylamino)sulfonyl,         di(C₁-C₆-alkyl)aminosulfonyl and (C₁-C₆-alkoxy)sulfonyl, where         the aliphatic and cycloaliphatic parts of the 17 aforementioned         radicals are unsubstituted, partly or completely halogenated,         -   phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl,             phenylaminosulfonyl, phenylaminocarbonyl,             phenyl(C₁-C₆-alkyl)aminocarbonyl, phenylcarbonyl and             phenoxycarbonyl,         -   wherein phenyl in the last 8 mentioned radicals are             unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or             different substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy             and C₁-C₆-haloalkoxy;     -   R² is selected from the group consisting of H, OH, S(O)₂NH₂, CN,         C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy, C₁-C₆-alkyl, C₂-C₆-alkenyl,         C₂-C₆-alkynyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)carbonyl,         (C₃-C₆-cycloalkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl,         (C₁-C₆-alkyl)sulfonyl, (C₁-C₆-alkylamino)carbonyl,         di(C₁-C₆-alkyl)aminocarbonyl, (C₁-C₆-alkylamino)sulfonyl,         di(C₁-C₆-alkyl)aminosulfonyl and (C₁-C₆-alkoxy)sulfonyl, where         the aliphatic and cycloaliphatic parts of the 17 aforementioned         radicals are unsubstituted, partly or completely halogenated,         -   phenyl, phenylsulfonyl, phenylaminosulfonyl, phenyl-C₁-C₆             alkyl, phenoxy, phenylaminocarbonyl,             phenyl(C₁-C₆-alkyl)aminocarbonyl, phenylcarbonyl and             phenoxycarbonyl,         -   wherein phenyl in the last 8 mentioned radicals is             unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or             different substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy             and C₁-C₆-haloalkoxy;     -   X is a radical selected from the group consisting of         -   CR³R⁴R⁵         -   phenyl, which is unsubstituted or carries 1, 2, 3, 4 or 5             radicals R^(Ar) which are identical or different;         -   NR^(3A)R^(3b),         -   OR³c and         -   S(O)_(k)R^(3d) with k being 0, 1 or 2,     -   wherein     -   R³ is selected from the group consisting of H, halogen, OH, CN,         C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkyl, C₃-C₆-cycloalkyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy, C₂-C₆-alkenyloxy,         C₂-C₆-alkynyloxy, C₃-C₆-cycloalkoxy,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy, where the aliphatic and         cycloaliphatic parts of the 9 aforementioned radicals are         unsubstituted, partly or completely halogenated;     -   R⁴ is selected from the group consisting of H, halogen, CN,         C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy;     -   R⁵ is selected from the group consisting of halogen, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₃-C₆-alkynyl, C₃-C₆-cycloalkyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₃-C₆-cycloalkenyl and         C₁-C₆-alkoxy-C₁-C₆-alkyl, where the aliphatic and cycloaliphatic         parts of the 7 aforementioned radicals are unsubstituted, partly         or completely halogenated;     -   R⁴ and R⁵ together with the carbon atom to which they are         attached form a moiety selected from the group consisting of         carbonyl, thiocarbonyl, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkenyl,         three- to six-membered saturated or partially unsaturated         heterocyclyl, and the moiety >C═CR^(x)R^(y), where R^(x) and         R^(y) are hydrogen, C₁-C₄-alkyl or C₁-C₄-haloalkyl;     -   R^(Ar) selected from the group consisting of halogen, OH, CN,         amino, NO₂, C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         C₁-C₆-alkoxy, C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,         C₁-C₆-alkylthio, (C₁-C₆-alkyl)sulfinyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)-carbonyl,         (C₁-C₆-alkyl)-carbonyloxy, C₃-C₆-cycloalkyl, C₃-C₆-cycloalkoxy,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy,         where the aliphatic and cycloaliphatic parts of the 22         aforementioned radicals are unsubstituted, partly or completely         halogenated and where the cycloaliphatic parts of the last 4         mentioned radicals may carry 1, 2, 3, 4, 5 or 6 methyl groups,     -   R^(3a), R^(3b), R^(3c) or R^(3d) are independently of one         another are selected from the group consisting of H, CN,         S(O)₂NH₂, C₁-C₆-alkyl, C₁-C₆-alkoxy, C₂-C₆-alkenyl,         C₂-C₆-alkynyl, C₃-C₆-cycloalkyl, (C₃-C₆-cycloalkyl)-C₁-C₆-alkyl,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,         (C₃-C₆-cycloalkyl)-carbonyl, (C₁-C₆-alkoxy)carbonyl,         (C₁-C₆-alkyl)sulfonyl, (C₁-C₆-alkylamino)carbonyl,         di(C₁-C₆-alkyl)aminocarbonyl, (C₁-C₆-alkylamino)sulfonyl,         di(C₁-C₆-alkyl)aminosulfonyl and (C₁-C₆-alkoxy)sulfonyl, where         the aliphatic and cycloaliphatic parts of the 16 aforementioned         radicals are unsubstituted, partly or completely halogenated,         phenyl, phenylsulfonyl, phenyl-C₁-C₆ alkyl, phenylaminosulfonyl,         phenylcarbonyl and phenoxycarbonyl, wherein phenyl in the last 6         mentioned radicals is unsubstituted or substituted by 1, 2, 3, 4         or 5 identical or different substituents selected from the group         consisting of halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl,         C₁-C₆-alkoxy and C₁-C₆-haloalkoxy, or     -   R^(3a), R^(3b) together with the nitrogen atom, to which they         are bound, form an N-bound, mono- or bicyclic heterocyclic         radical, which may have 1, 2, 3 or 4 further heteroatoms which         are selected from N, O and S, which is unsubstituted or         substituted by one or more identical or different substituents         selected from the group consisting of halogen, CN, NO₂,         C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy,         -   one of R^(3a), R^(3b) may also be OH, C₁-C₆-alkoxy,             C₃-C₆-cycloalkoxy, (C₃-C₆-cycloalkyl)-C₁-C₄-alkoxy,             C₂-C₆-alkenyloxy, C₂-C₆-alkynyloxy,             (C₁-C₆-alkoxy)-C₁-C₆-alkoxy, where the aliphatic and             cycloaliphatic parts of the 6 aforementioned radicals are             unsubstituted, partly or completely halogenated,         -   or phenoxy, which is unsubstituted or substituted by 1, 2,             3, 4 or 5 identical or different substituents selected from             the group consisting of halogen, CN, NO₂, C₁-C₆-alkyl,             C₁-C₆-haloalkyl, C₁-C₆-alkoxy and C₁-C₆-haloalkoxy;     -   including their agriculturally acceptable salts.

In another preferred embodiment, the azines useful for the present invention comprise a diaminotriazine compound of formula (I)

-   -   wherein     -   A is phenyl, which is substituted by fluorine in the         ortho-position and which may additionally carry 1, 2, 3 or 4         identical or different substituents R^(A) selected from the         group consisting of halogen, OH, CN, amino, NO₂, C₁-C₆-alkyl,         C₂-C₆-alkenyl, C₂-C₆-alkynyl, (C₁-C₆-alkoxy)-C₂-C₆-alkyl,         (C₁-C₆-alkoxy)-C₂-C₆-alkenyl, (C₁-C₆-alkoxy)-C₂-C₆-alkynyl,         (C₁-C₆-alkyl)amino, di(C₁-C₆-alkyl)amino,         (C₁-C₆-alkyl)-carbonyl, (C₁-C₆-alkoxy)-carbonyl,         C₃-C₆-cycloalkyl, (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, where the         aliphatic and cycloaliphatic parts of the 11 aforementioned         radicals are unsubstituted, partly or completely halogenated and         where the cycloaliphatic parts of the last 4 mentioned radicals         may carry 1, 2, 3, 4, 5 or 6 methyl groups;     -   R¹ is selected from the group consisting of H, OH, S(O)₂NH₂, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)-carbonyl,         (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,         (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and         (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic         parts of the 14 aforementioned radicals are unsubstituted,         partly or completely halogenated,         -   phenyl, phenyl-C₁-C₆-alkyl, phenylsulfonyl,             phenylaminosulfonyl, phenylcarbonyl and phenoxycarbonyl,         -   wherein phenyl in the last 6 mentioned radicals are             unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or             different substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy             and C₁-C₆-haloalkoxy;     -   R² is selected from the group consisting of H, OH, S(O)₂NH₂, CN,         C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,         (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, C₁-C₆-alkoxy,         (C₁-C₆-alkoxy)-C₁-C₆-alkyl, (C₁-C₆-alkyl)carbonyl,         (C₁-C₆-alkoxy)carbonyl, (C₁-C₆-alkyl)sulfonyl,         (C₁-C₆-alkylamino)carbonyl, di(C₁-C₆-alkyl)aminocarbonyl,         (C₁-C₆-alkylamino)sulfonyl, di(C₁-C₆-alkyl)aminosulfonyl and         (C₁-C₆-alkoxy)sulfonyl, where the aliphatic and cycloaliphatic         parts of the 14 aforementioned radicals are unsubstituted,         partly or completely halogenated,         -   phenyl, phenylsulfonyl, phenylaminosulfonyl, phenyl-C₁-C₆             alkyl, phenoxy, phenylcarbonyl and phenoxycarbonyl,         -   wherein phenyl in the last 6 mentioned radicals is             unsubstituted or substituted by 1, 2, 3, 4 or 5 identical or             different substituents selected from the group consisting of             halogen, CN, NO₂, C₁-C₆-alkyl, C₁-C₆-haloalkyl, C₁-C₆-alkoxy             and C₁-C₆-haloalkoxy; R³ is selected from the group             consisting of C₁-C₆-alkyl, C₂-C₆-alkenyl, C₂-C₆-alkynyl,             (C₃-C₆-cycloalkyl)-C₁-C₄-alkyl, (C₁-C₆-alkoxy)-C₁-C₆-alkyl,             (C₁-C₆-alkyl)carbonyl, (C₁-C₆-alkoxy)carbonyl,             (C₁-C₆-alkylamino)carbonyl and di(C₁-C₆-alkyl)aminocarbonyl,             where the aliphatic and cycloaliphatic parts of the 9             aforementioned radicals are unsubstituted, partly or             completely halogenated,     -   R⁴ and R⁵, together with the carbon atom, to which they are         bound form a saturated 3-, 4-, 5- or 6-membered carbocyclic         radical or a saturated 3-, 4-, 5- or 6-membered heterocyclic         radical, where the carbocyclic radical and the heterocyclic         radical are unsubstituted, partly or completely halogenated or         carry from 1 to 6 C₁-C₆-alkyl groups;     -   including their agriculturally acceptable salts.

The herbicidal compounds (component A) useful for the present invention as disclosed SUPRA may further be used in conjunction with additional herbicides to which the crop plant is naturally tolerant or to which has been made tolerant by mutagenesis as described SUPRA, or to which it is resistant via expression of one or more additional transgenes as mentioned supra. The CESA-inhibiting herbicides useful for the present invention are often best applied in conjunction with one or more other herbicides to obtain control of a wider variety of undesirable vegetation. When used in conjunction with other herbicides (hereinafter referred to a compound B), the presently claimed compounds can be formulated with the other herbicide or herbicides, tank mixed with the other herbicide or herbicides, or applied sequentially with the other herbicide or herbicides.

The further herbicidal compound B (component B) is in particular selected from the herbicides of class b1) to b15):

-   b1) lipid biosynthesis inhibitors; -   b2) acetolactate synthase inhibitors (ALS inhibitors); -   b3) photosynthesis inhibitors; -   b4) protoporphyrinogen-IX oxidase inhibitors, -   b5) bleacher herbicides; -   b6) enolpyruvyl shikimate 3-phosphate synthase inhibitors (EPSP     inhibitors); -   b7) glutamine synthetase inhibitors; -   b8) 7,8-dihydropteroate synthase inhibitors (DHP inhibitors); -   b9) mitosis inhibitors; -   b10) inhibitors of the synthesis of very long chain fatty acids     (VLCFA inhibitors); -   b11) cellulose biosynthesis inhibitors; -   b12) decoupler herbicides; -   b13) auxinic herbicides; -   b14) auxin transport inhibitors; and -   b15) other herbicides selected from the group consisting of     bromobutide, chlorflurenol, chlorflurenol-methyl, cinmethylin,     cumyluron, dalapon, dazomet, difenzoquat, difenzoquat-metilsulfate,     dimethipin, DSMA, dymron, endothal and its salts, etobenzanid,     flamprop, flamprop-isopropyl, flamprop-methyl, flamprop-M-isopropyl,     flamprop-M-methyl, flurenol, flurenol-butyl, flurprimidol, fosamine,     fosamine-ammonium, indanofan, indaziflam, maleic hydrazide,     mefluidide, metam, methiozolin (CAS 403640-27-7), methyl azide,     methyl bromide, methyl-dymron, methyl iodide, MSMA, oleic acid,     oxaziclomefone, pelargonic acid, pyributicarb, quinoclamine,     triaziflam, tridiphane and     6-chloro-3-(2-cyclopropyl-6-methylphenoxy)-4-pyridazinol (CAS     499223-49-3) and its salts and esters;     -   including their agriculturally acceptable salts or derivatives         such as ethers, esters or amides.     -   Preference is given to those compositions according to the         present invention comprising at least one herbicide B selected         from herbicides of class b1, b6, b9, b10 and b11.     -   Examples of herbicides B which can be used in combination with         the compounds of formula (I) according to the present invention         are:     -   b1) from the group of the lipid biosynthesis inhibitors:         ACC-herbicides such as alloxydim, alloxydim-sodium, butroxydim,         clethodim, clodinafop, clodinafop-propargyl, cycloxydim,         cyhalofop, cyhalofop-butyl, diclofop, diclofop-methyl,         fenoxaprop, fenoxaprop-ethyl, fenoxaprop-P, fenoxaprop-P-ethyl,         fluazifop, fluazifop-butyl, fluazifop-P, fluazifop-P-butyl,         haloxyfop, haloxyfop-methyl, haloxyfop-P, haloxyfop-P-methyl,         metamifop, pinoxaden, profoxydim, propaquizafop, quizalofop,         quizalofop-ethyl, quizalofop-tefuryl, quizalofop-P,         quizalofop-P-ethyl, quizalofop-P-tefuryl, sethoxydim,         tepraloxydim, tralkoxydim,         4-(4′-Chloro-4-cyclopropyl-2′-fluoro[1,1′-biphenyl]-3-yl)-5-hydroxy-2,2,6,6-tetramethyl-2H-pyran-3(6H)-one         (CAS 1312337-72-6);         4-(2′,4′-Dichloro-4-cyclopropyl[1,1′-biphenyl]-3-yl)-5-hydroxy-2,2,6,6-tetramethyl-2H-pyran-3(6H)-one         (CAS 1312337-45-3);         4-(4′-Chloro-4-ethyl-2′-fluoro[1,1′-biphenyl]-3-yl)-5-hydroxy-2,2,6,6-tetramethyl-2H-pyran-3(6H)-one         (CAS 1033757-93-5);         4-(2′,4′-Dichloro-4-ethyl[1,1′-biphenyl]-3-yl)-2,2,6,6-tetramethyl-2H-pyran-3,5(4H,6H)-dione         (CAS 1312340-84-3);         5-(Acetyloxy)-4-(4′-chloro-4-cyclopropyl-2′-fluoro[1,1′-biphenyl]-3-yl)-3,6-dihydro-2,2,6,6-tetramethyl-2H-pyran-3-one         (CAS 1312337-48-6);         5-(Acetyloxy)-4-(2′,4′-dichloro-4-cyclopropyl-[1,1′-biphenyl]-3-yl)-3,6-dihydro-2,2,6,6-tetramethyl-2H-pyran-3-one;         5-(Acetyloxy)-4-(4′-chloro-4-ethyl-2′-fluoro[1,1′-biphenyl]-3-yl)-3,6-dihydro-2,2,6,6-tetramethyl-2H-pyran-3-one         (CAS 1312340-82-1);         5-(Acetyloxy)-4-(2′,4′-dichloro-4-ethyl[1,1′-biphenyl]-3-yl)-3,6-dihydro-2,2,6,6-tetra         methyl-2H-pyran-3-one (CAS 1033760-55-2);         4-(4′-Chloro-4-cyclopropyl-2′-fluoro[1,1′-biphenyl]-3-yl)-5,6-dihydro-2,2,6,6-tetramethyl-5-oxo-2H-pyran-3-yl         carbonic acid methyl ester (CAS 1312337-51-1);         4-(2′,4′-Dichloro-4-cyclopropyl-[1,1′-biphenyl]-3-yl)-5,6-dihydro-2,2,6,6-tetramethyl-5-oxo-2H-pyran-3-yl         carbonic acid methyl ester;         4-(4′-Chloro-4-ethyl-2′-fluoro[1,1′-biphenyl]-3-yl)-5,6-dihydro-2,2,6,6-tetramethyl-5-oxo-2H-pyran-3-yl         carbonic acid methyl ester (CAS 1312340-83-2);         4-(2′,4′-Dichloro-4-ethyl[1,1′-biphenyl]-3-yl)-5,6-dihydro-2,2,6,6-tetramethyl-5-oxo-2H-pyran-3-yl         carbonic acid methyl ester (CAS 1033760-58-5); and non ACC         herbicides such as benfuresate, butylate, cycloate, dalapon,         dimepiperate, EPTC, esprocarb, ethofumesate, flupropanate,         molinate, orbencarb, pebulate, prosulfocarb, TCA, thiobencarb,         tiocarbazil, triallate and vernolate;     -   b2) from the group of the ALS inhibitors:         sulfonylureas such as amidosulfuron, azimsulfuron, bensulfuron,         bensulfuron-methyl, chlorimuron, chlorimuron-ethyl,         chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron,         ethametsulfuron-methyl, ethoxysulfuron, flazasulfuron,         flucetosulfuron, flupyrsulfuron, flupyrsulfuron-methyl-sodium,         foramsulfuron, halosulfuron, halosulfuron-methyl, imazosulfuron,         iodosulfuron, iodosulfuron-methyl-sodium, iofensulfuron,         iofensulfuron-sodium, mesosulfuron, metazosulfuron, metsulfuron,         metsulfuron-methyl, nicosulfuron, orthosulfamuron, oxasulfuron,         primisulfuron, primisulfuron-methyl, propyrisulfuron,         prosulfuron, pyrazosulfuron, pyrazosulfuron-ethyl, rimsulfuron,         sulfometuron, sulfometuron-methyl, sulfosulfuron,         thifensulfuron, thifensulfuron-methyl, triasulfuron, tribenuron,         tribenuron-methyl, trifloxysulfuron, triflusulfuron,         triflusulfuron-methyl and tritosulfuron,         imidazolinones such as imazamethabenz, imazamethabenz-methyl,         imazamox, imazapic, imazapyr, imazaquin and imazethapyr,         triazolopyrimidine herbicides and sulfonanilides such as         cloransulam, cloransulam-methyl, diclosulam, flumetsulam,         florasulam, metosulam, penoxsulam, pyrimisulfan and pyroxsulam,         pyrimidinylbenzoates such as bispyribac, bispyribac-sodium,         pyribenzoxim, pyriftalid, pyriminobac, pyriminobac-methyl,         pyrithiobac, pyrithiobac-sodium,         4-[[[2-[(4,6-dimethoxy-2-pyrimidinyl)oxy]phenyl]methyl]amino]-benzoic         acid-1-methylethyl ester (CAS 420138-41-6),         4-[[[2-[(4,6-dimethoxy-2-pyrimidinyl)oxy]phenyl]methyl]amino]-benzoic         acid propyl ester (CAS 420138-40-5),         N-(4-bromophenyl)-2-[(4,6-dimethoxy-2-pyrimidinyl)oxy]benzenemethanamine         (CAS 420138-01-8),         sulfonylaminocarbonyl-triazolinone herbicides such as         flucarbazone, flucarbazone-sodium, propoxycarbazone,         propoxycarbazone-sodium, thiencarbazone and         thiencarbazone-methyl; and triafamone;         among these, a preferred embodiment of the invention relates to         those compositions comprising at least one imidazolinone         herbicide;     -   b3) from the group of the photosynthesis inhibitors:         amicarbazone, inhibitors of the photosystem II, e.g. triazine         herbicides, including of chlorotriazine, triazinones,         triazindiones, methylthiotriazines and pyridazinones such as         ametryn, atrazine, chloridazone, cyanazine, desmetryn,         dimethametryn, hexazinone, metribuzin, prometon, prometryn,         propazine, simazine, simetryn, terbumeton, terbuthylazin,         terbutryn and trietazin, aryl urea such as chlorobromuron,         chlorotoluron, chloroxuron, dimefuron, diuron, fluometuron,         isoproturon, isouron, linuron, metamitron, methabenzthiazuron,         metobenzuron, metoxuron, monolinuron, neburon, siduron,         tebuthiuron and thiadiazuron, phenyl carbamates such as         desmedipham, karbutilat, phenmedipham, phenmedipham-ethyl,         nitrile herbicides such as bromofenoxim, bromoxynil and its         salts and esters, ioxynil and its salts and esters, uraciles         such as bromacil, lenacil and terbacil, and bentazon and         bentazon-sodium, pyridate, pyridafol, pentanochlor and propanil         and inhibitors of the photosystem I such as diquat,         diquat-dibromide, paraquat, paraquat-dichloride and         paraquat-dimetilsulfate. Among these, a preferred embodiment of         the invention relates to those compositions comprising at least         one aryl urea herbicide. Among these, likewise a preferred         embodiment of the invention relates to those compositions         comprising at least one triazine herbicide. Among these,         likewise a preferred embodiment of the invention relates to         those compositions comprising at least one nitrile herbicide;     -   b4) from the group of the protoporphyrinogen-IX oxidase         inhibitors:         acifluorfen, acifluorfen-sodium, azafenidin, bencarbazone,         benzfendizone, bifenox, butafenacil, carfentrazone,         carfentrazone-ethyl, chlomethoxyfen, cinidon-ethyl, fluazolate,         flufenpyr, flufenpyr-ethyl, flumiclorac, flumiclorac-pentyl,         flumioxazin, fluoroglycofen, fluoroglycofen-ethyl, fluthiacet,         fluthiacet-methyl, fomesafen, halosafen, lactofen, oxadiargyl,         oxadiazon, oxyfluorfen, pentoxazone, profluazol, pyraclonil,         pyraflufen, pyraflufen-ethyl, saflufenacil, sulfentrazone,         thidiazimin, tiafenacil, ethyl         [3-[2-chloro-4-fluoro-5-(1-methyl-6-trifluoromethyl-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-3-yl)phenoxy]-2-pyridyloxy]acetate         (CAS 353292-31-6; S-3100),         N-ethyl-3-(2,6-dichloro-4-trifluoromethylphenoxy)-5-methyl-1H-pyrazole-1-carboxamide         (CAS 452098-92-9),         N-tetrahydrofurfuryl-3-(2,6-dichloro-4-trifluoromethylphenoxy)-5-methyl-1H-pyrazole-1-carboxamide         (CAS 915396-43-9),         N-ethyl-3-(2-chloro-6-fluoro-4-trifluoromethylphenoxy)-5-methyl-1H-pyrazole-1-carboxamide         (CAS 452099-05-7),         N-tetrahydrofurfuryl-3-(2-chloro-6-fluoro-4-trifluoromethylphenoxy)-5-methyl-1H-pyrazole-1-carboxamide         (CAS 452100-03-7),         3-[7-fluoro-3-oxo-4-(prop-2-ynyl)-3,4-dihydro-2H-benzo[1,4]oxazin-6-yl]-1,5-dimethyl-6-thioxo-[1,3,5]triazinan-2,4-dione,         1,5-dimethyl-6-thioxo-3-(2,2,7-trifluoro-3-oxo-4-(prop-2-ynyl)-3,4-dihydro-2H-benzo[b][1,4]oxazin-6-yl)-1,3,5-triazinane-2,4-dione         (CAS 1258836-72-4),         2-(2,2,7-Trifluoro-3-oxo-4-prop-2-ynyl-3,4-dihydro-2H-benzo[1,4]oxazin-6-yl)-4,5,6,7-tetrahydro-isoindole-1,3-dione,         1-Methyl-6-trifluoromethyl-3-(2,2,7-trifluoro-3-oxo-4-prop-2-ynyl-3,4-dihydro-2H-benzo[1,4]oxazin-6-yl)-1H-pyrimidine-2,4-dione,         methyl         (E)-4-[2-chloro-5-[4-chloro-5-(difluoromethoxy)-1H-methyl-pyrazol-3-yl]-4-fluoro-phenoxy]-3-methoxy-but-2-enoate         [CAS 948893-00-3], and         3-[7-Chloro-5-fluoro-2-(trifluoromethyl)-1H-benzimidazol-4-yl]-1-methyl-6-(trifluoromethyl)-1H-pyrimidine-2,4-dione         (CAS 212754-02-4);     -   b5) from the group of the bleacher herbicides:         PDS inhibitors: beflubutamid, diflufenican, fluridone,         flurochloridone, flurtamone, norflurazon, picolinafen, and         4-(3-trifluoromethylphenoxy)-2-(4-trifluoromethylphenyl)pyrimidine         (CAS 180608-33-7), HPPD inhibitors: benzobicyclon, benzofenap,         clomazone, fenquintrione, isoxaflutole, mesotrione,         pyrasulfotole, pyrazolynate, pyrazoxyfen, sulcotrione,         tefuryltrione, tembotrione, topramezone and bicyclopyrone,         bleacher, unknown target: aclonifen, amitrole and flumeturon;     -   b6) from the group of the EPSP synthase inhibitors:         glyphosate, glyphosate-isopropylammonium, glyposate-potassium         and glyphosate-trimesium (sulfosate);     -   b7) from the group of the glutamine synthase inhibitors:         bilanaphos (bialaphos), bilanaphos-sodium, glufosinate,         glufosinate-P and glufosinate-ammonium;     -   b8) from the group of the DHP synthase inhibitors:         asulam;     -   b9) from the group of the mitosis inhibitors:         compounds of group K1: dinitroanilines such as benfluralin,         butralin, dinitramine, ethalfluralin, fluchloralin, oryzalin,         pendimethalin, prodiamine and trifluralin, phosphoramidates such         as amiprophos, amiprophos-methyl, and butamiphos, benzoic acid         herbicides such as chlorthal, chlorthal-dimethyl, pyridines such         as dithiopyr and thiazopyr, benzamides such as propyzamide and         tebutam; compounds of group K2: chlorpropham, propham and         carbetamide, among these, compounds of group K1, in particular         dinitroanilines are preferred;     -   b10) from the group of the VLCFA inhibitors:         chloroacetamides such as acetochlor, alachlor, butachlor,         dimethachlor, dimethenamid, dimethenamid-P, metazachlor,         metolachlor, metolachlor-S, pethoxamid, pretilachlor,         propachlor, propisochlor and thenylchlor, oxyacetanilides such         as flufenacet and mefenacet, acetanilides such as diphenamid,         naproanilide, napropamide and napropamide-M, tetrazolinones such         fentrazamide, and other herbicides such as anilofos,         cafenstrole, fenoxasulfone, ipfencarbazone, piperophos,         pyroxasulfone and isoxazoline compounds of the formulae II.1,         II.2, II.3, II.4, II.5, II.6, II.7, II.8 and II.9

the isoxazoline compounds of the formula (I)I are known in the art, e.g. from WO 2006/024820, WO 2006/037945, WO 2007/071900 and WO 2007/096576; among the VLCFA inhibitors, preference is given to chloroacetamides and oxyacetamides;

-   -   b11) from the group of the cellulose biosynthesis inhibitors:         chlorthiamid, dichlobenil, flupoxam, isoxaben and         1-Cyclohexyl-5-pentafluorphenyloxy-1⁴-[1,2,4,6]thiatriazin-3-ylamine;     -   b12) from the group of the decoupler herbicides:         dinoseb, dinoterb and DNOC and its salts;     -   b13) from the group of the auxinic herbicides:         2,4-D and its salts and esters such as clacyfos, 2,4-DB and its         salts and esters, aminocyclopyrachlor and its salts and esters,         aminopyralid and its salts such as         aminopyralid-dimethylammonium,         aminopyralid-tris(2-hydroxypropyl)ammonium and its esters,         benazolin, benazolin-ethyl, chloramben and its salts and esters,         clomeprop, clopyralid and its salts and esters, dicamba and its         salts and esters, dichlorprop and its salts and esters,         dichlorprop-P and its salts and esters, fluroxypyr,         fluroxypyr-butometyl, fluroxypyr-meptyl, halauxifen and its         salts and esters (CAS 943832-60-8); MCPA and its salts and         esters, MCPA-thioethyl, MCPB and its salts and esters, mecoprop         and its salts and esters, mecoprop-P and its salts and esters,         picloram and its salts and esters, quinclorac, quinmerac, TBA         (2,3,6) and its salts and esters and triclopyr and its salts and         esters;     -   b14) from the group of the auxin transport inhibitors:         diflufenzopyr, diflufenzopyr-sodium, naptalam and         naptalam-sodium;     -   b15) from the group of the other herbicides: bromobutide,         chlorflurenol, chlorflurenol-methyl, cinmethylin, cumyluron,         cyclopyrimorate (CAS 499223-49-3) and its salts and esters,         dalapon, dazomet, difenzoquat, difenzoquat-metilsulfate,         dimethipin, DSMA, dymron, endothal and its salts, etobenzanid,         flamprop, flamprop-isopropyl, flamprop-methyl,         flamprop-M-isopropyl, flamprop-M-methyl, flurenol,         flurenol-butyl, flurprimidol, fosamine, fosamine-ammonium,         indanofan, indaziflam, maleic hydrazide, mefluidide, metam,         methiozolin (CAS 403640-27-7), methyl azide, methyl bromide,         methyl-dymron, methyl iodide, MSMA, oleic acid, oxaziclomefone,         pelargonic acid, pyributicarb, quinoclamine, triaziflam and         tridiphane.

Active compounds B and C having a carboxyl group can be employed in the form of the acid, in the form of an agriculturally suitable salt as mentioned above or else in the form of an agriculturally acceptable derivative in the compositions according to the invention.

In the case of dicamba, suitable salts include those, where the counterion is an agriculturally acceptable cation. For example, suitable salts of dicamba are dicamba-sodium, dicamba-potassium, dicamba-methylammonium, dicamba-dimethylammonium, dicamba-isopropylammonium, dicamba-diglycolamine, dicamba-olamine, dicamba-diolamine, dicamba-trolamine, dicamba-N,N-bis-(3-aminopropyl)methylamine and dicamba-diethylenetriamine. Examples of a suitable ester are dicamba-methyl and dicamba-butotyl.

Suitable salts of 2,4-D are 2,4-D-ammonium, 2,4-D-dimethylammonium, 2,4-D-diethylammonium, 2,4-D-diethanolammonium (2,4-D-diolamine), 2,4-D-triethanol-ammonium, 2,4-D-isopropylammonium, 2,4-D-triisopropanolammonium, 2,4-D-heptylammonium, 2,4-D-dodecylammonium, 2,4-D-tetradecylammonium, 2,4-D-triethylammonium, 2,4-D-tris(2-hydroxypropyl)ammonium, 2,4-D-tris(isopropyl)ammonium, 2,4-D-trolamine, 2,4-D-lithium, 2,4-D-sodium. Examples of suitable esters of 2,4-D are 2,4-D-butotyl, 2,4-D-2-butoxypropyl, 2,4-D-3-butoxypropyl, 2,4-D-butyl, 2,4-D-ethyl, 2,4-D-ethylhexyl, 2,4-D-isobutyl, 2,4-D-isooctyl, 2,4-D-isopropyl, 2,4-D-meptyl, 2,4-D-methyl, 2,4-D-octyl, 2,4-D-pentyl, 2,4-D-propyl, 2,4-D-tefuryl and clacyfos.

Suitable salts of 2,4-DB are for example 2,4-DB-sodium, 2,4-DB-potassium and 2,4-DB-dimethylammonium. Suitable esters of 2,4-DB are for example 2,4-DB-butyl and 2,4-DB-isoctyl.

Suitable salts of dichlorprop are for example dichlorprop-sodium, dichlorprop-potassium and dichlorprop-dimethylammonium. Examples of suitable esters of dichlorprop are dichlorprop-butotyl and dichlorprop-isoctyl.

Suitable salts and esters of MCPA include MCPA-butotyl, MCPA-butyl, MCPA-dimethylammonium, MCPA-diolamine, MCPA-ethyl, MCPA-thioethyl, MCPA-2-ethylhexyl, MCPA-isobutyl, MCPA-isoctyl, MCPA-isopropyl, MCPA-isopropylammonium, MCPA-methyl, MCPA-olamine, MCPA-potassium, MCPA-sodium and MCPA-trolamine.

A suitable salt of MCPB is MCPB sodium. A suitable ester of MCPB is MCPB-ethyl.

Suitable salts of clopyralid are clopyralid-potassium, clopyralid-olamine and clopyralid-tris-(2-hydroxypropyl)ammonium. Example of suitable esters of clopyralid is clopyralid-methyl.

Examples of a suitable ester of fluroxypyr are fluroxypyr-meptyl and fluroxypyr-2-butoxy-1-methylethyl, wherein fluroxypyr-meptyl is preferred.

Suitable salts of picloram are picloram-dimethylammonium, picloram-potassium, picloram-triisopropanolammonium, picloram-triisopropylammonium and picloram-trolamine. A suitable ester of picloram is picloram-isoctyl.

A suitable salt of triclopyr is triclopyr-triethylammonium. Suitable esters of triclopyr are for example triclopyr-ethyl and triclopyr-butotyl.

Suitable salts and esters of chloramben include chloramben-ammonium, chloramben-diolamine, chloramben-methyl, chloramben-methylammonium and chloramben-sodium.

Suitable salts and esters of 2,3,6-TBA include 2,3,6-TBA-dimethylammonium, 2,3,6-TBA-lithium, 2,3,6-TBA-potassium and 2,3,6-TBA-sodium.

Suitable salts and esters of aminopyralid include aminopyralid-potassium, aminopyralid-dimethylammonium, and aminopyralid-tris(2-hydroxypropyl)ammonium.

Suitable salts of glyphosate are for example glyphosate-ammonium, glyphosate-diammonium, glyphoste-dimethylammonium, glyphosate-isopropylammonium, glyphosate-potassium, glyphosate-sodium, glyphosate-trimesium as well as the ethanolamine and diethanolamine salts, preferably glyphosate-diammonium, glyphosate-isopropylammonium and glyphosate-trimesium (sulfosate).

A suitable salt of glufosinate is for example glufosinate-ammonium.

A suitable salt of glufosinate-P is for example glufosinate-P-ammonium.

Suitable salts and esters of bromoxynil are for example bromoxynil-butyrate, bromoxynil-heptanoate, bromoxynil-octanoate, bromoxynil-potassium and bromoxynil-sodium.

Suitable salts and esters of ioxonil are for example ioxonil-octanoate, ioxonil-potassium and ioxonil-sodium.

Suitable salts and esters of mecoprop include mecoprop-butotyl, mecoprop-dimethylammonium, mecoprop-diolamine, mecoprop-ethadyl, mecoprop-2-ethylhexyl, mecoprop-isoctyl, mecoprop-methyl, mecoprop-potassium, mecoprop-sodium and mecoprop-trolamine.

Suitable salts of mecoprop-P are for example mecoprop-P-butotyl, mecoprop-P-dimethylammonium, mecoprop-P-2-ethylhexyl, mecoprop-P-isobutyl, mecoprop-P-potassium and mecoprop-P-sodium.

A suitable salt of diflufenzopyr is for example diflufenzopyr-sodium.

A suitable salt of naptalam is for example naptalam-sodium.

Suitable salts and esters of aminocyclopyrachlor are for example aminocyclopyrachlor-dimethylammonium, aminocyclopyrachlor-methyl, aminocyclopyrachlor-triisopropanolammonium, aminocyclopyrachlor-sodium and aminocyclopyrachlor-potassium.

A suitable salt of quinclorac is for example quinclorac-dimethylammonium.

A suitable salt of quinmerac is for example quinclorac-dimethylammonium.

A suitable salt of imazamox is for example imazamox-ammonium.

Suitable salts of imazapic are for example imazapic-ammonium and imazapic-isopropylammonium.

Suitable salts of imazapyr are for example imazapyr-ammonium and imazapyr-isopropylammonium.

A suitable salt of imazaquin is for example imazaquin-ammonium.

Suitable salts of imazethapyr are for example imazethapyr-ammonium and imazethapyr-isopropylammonium.

A suitable salt of topramezone is for example topramezone-sodium.

Particularly preferred herbicidal compounds B are the herbicides B as defined above; in particular the herbicides B.1-B.189 listed below in table B:

TABLE B Herbicide B B.1 clethodim B.2 clodinafop-propargyl B.3 cycloxydim B.4 cyhalofop-butyl B.5 fenoxaprop-ethyl B.6 fenoxaprop-P-ethyl B.7 metamifop B.8 pinoxaden B.9 profoxydim B.10 sethoxydim B.11 tepraloxydim B.12 tralkoxydim B.13 esprocarb B.14 ethofumesate B.15 molinate B.16 prosulfocarb B.17 thiobencarb B.18 triallate B.19 bensulfuron-methyl B.20 bispyribac-sodium B.21 cloransulam-methyl B.22 chlorsulfuron B.23 clorimuron B.24 cyclosulfamuron B.25 diclosulam B.26 florasulam B.27 flumetsulam B.28 flupyrsulfuron-methyl-sodium B.29 foramsulfuron B.30 imazamox B.31 imazamox-ammonium B.32 imazapic B.33 imazapic-ammonium B.34 imazapic-isopropylammonium B.35 imazapyr B.36 imazapyr-ammonium B.37 imazapyr-isopropylammonium B.38 imazaquin B.39 imazaquin-ammonium B.40 imazethapyr B.41 imazethapyr-ammonium B.42 imazethapyr- isopropylammonium B.43 imazosulfuron B.44 iodosulfuron-methyl-sodium B.45 iofensulfuron B.46 iofensulfuron-sodium B.47 mesosulfuron-methyl B.48 metazosulfuron B.49 metsulfuron-methyl B.50 metosulam B.51 nicosulfuron B.52 penoxsulam B.53 propoxycarbazon-sodium B.54 pyrazosulfuron-ethyl B.55 pyribenzoxim B.56 pyriftalid B.57 pyroxsulam B.58 propyrisulfuron B.59 rimsulfuron B.60 sulfosulfuron B.61 thiencarbazone-methyl B.62 thifensulfuron-methyl B.63 tribenuron-methyl B.64 tritosulfuron B.65 triafamone B.66 ametryne B.67 atrazine B.68 bentazon B.69 bromoxynil B.70 bromoxynil-octanoate B.71 bromoxynil-heptanoate B.72 bromoxynil-potassium B.73 diuron B.74 fluometuron B.75 hexazinone B.76 isoproturon B.77 linuron B.78 metamitron B.79 metribuzin B.80 propanil B.81 simazin B.82 terbuthylazine B.83 terbutryn B.84 paraquat-dichloride B.85 acifluorfen B.86 butafenacil B.87 carfentrazone-ethyl B.88 flumioxazin B.89 fomesafen B.90 oxadiargyl B.91 oxyfluorfen B.92 saflufenacil B.93 sulfentrazone B.94 ethyl [3-[2-chloro-4-fluoro-5- (1-methyl-6-trifluoromethyl- 2,4-dioxo-1,2,3,4-tetrahydro- pyrimidin-3-yl)phenoxy]-2- pyridyloxy]acetate (CAS 353292-31-6) B.95 1,5-dimethyl-6-thioxo-3-(2,2,7- trifluoro-3-oxo-4-(prop-2- ynyl)-3,4-dihydro-2H- benzo[b][1,4]oxazin-6-yl)-1,3,5- triazinane-2,4-dione (CAS 1258836-72-4) B.96 benzobicyclon B.97 clomazone B.98 diflufenican B.99 flurochloridone B.100 isoxaflutole B.101 mesotrione B.102 norflurazone B.103 picolinafen B.104 sulcotrione B.105 tefuryltrione B.106 tembotrione B.107 topramezone B.108 topramezone-sodium B.109 bicyclopyrone B.110 amitrole B.111 fluometuron B.112 fenquintrione B.113 glyphosate B.114 glyphosate-ammonium B.115 glyphosate- dimethylammonium B.116 glyphosate- isopropylammonium B.117 glyphosate-trimesium (sulfosate) B.118 glyphosate-potassium B.119 glufosinate B.120 glufosinate-ammonium B.121 glufosinate-P B.122 glufosinate-P-ammonium B.123 pendimethalin B.124 trifluralin B.125 acetochlor B.126 butachlor B.127 cafenstrole B.128 dimethenamid-P B.129 fentrazamide B.130 flufenacet B.131 mefenacet B.132 metazachlor B.133 metolachlor B.134 S-metolachlor B.135 pretilachlor B.136 fenoxasulfone B.137 isoxaben B.138 ipfencarbazone B.139 pyroxasulfone B.140 2,4-D B.141 2,4-D-isobutyl B.142 2,4-D-dimethylammonium B.143 2,4-D-N,N,N- trimethylethanolammonium B.144 aminopyralid B.145 aminopyralid-methyl B.146 aminopyralid-dimethyl- ammonium B.147 aminopyralid-tris(2- hydroxpropyl)ammonium B.148 clopyralid B.149 clopyralid-methyl B.150 clopyralid-olamine B.151 dicamba B.152 dicamba-butotyl B.153 dicamba-diglycolamine B.154 dicamba-dimethylammonium B.155 dicamba-diolamine B.156 dicamba-isopropylammonium B.157 dicamba-potassium B.158 dicamba-sodium B.159 dicamba-trolamine B.160 dicamba-N,N-bis-(3- aminopropyl)methylamine B.161 dicamba-diethylenetriamine B.162 fluroxypyr B.163 fluroxypyr-meptyl B.164 MCPA B.165 MCPA-2-ethylhexyl B.166 MCPA-dimethylammonium B.167 quinclorac B.168 quinclorac- dimethylammonium B.169 quinmerac B.170 quinmerac- dimethylammonium B.171 aminocyclopyrachlor B.172 aminocyclopyrachlor-potassium B.173 aminocyclopyrachlor-methyl B.174 diflufenzopyr B.175 diflufenzopyr-sodium B.176 dymron B.177 indanofan B.178 indaziflam B.179 oxaziclomefone B.180 triaziflam B.181 II.1 B.182 II.2 B.183 II.3 B.184 II.4 B.185 II.5 B.186 II.6 B.187 II.7 B.188 II.8 B.189 II.9

Moreover, it may be useful to apply the compounds of formula (I) in combination with safeners and optionally with one or more further herbicides. Safeners are chemical compounds which prevent or reduce damage on useful plants without having a major impact on the herbicidal action of the compounds of the formula (I) towards unwanted plants. They can be applied either before sowings (e.g. on seed treatments, shoots or seedlings) or in the pre-emergence application or post-emergence application of the useful plant. The safeners and the compounds of formula (I) and optionally the herbicides B can be applied simultaneously or in succession.

Suitable safeners are e.g. (quinolin-8-oxy)acetic acids, 1-phenyl-5-haloalkyl-1H-1,2,4-triazol-3-carboxylic acids, 1-phenyl-4,5-dihydro-5-alkyl-1H-pyrazol-3,5-dicarboxylic acids, 4,5-dihydro-5,5-diaryl-3-isoxazol carboxylic acids, dichloroacetamides, alpha-oximinophenylacetonitriles, acetophenonoximes, 4,6-dihalo-2-phenylpyrimidines, N-[[4-(aminocarbonyl)phenyl]sulfonyl]-2-benzoic amides, 1,8-naphthalic anhydride, 2-halo-4-(haloalkyl)-5-thiazol carboxylic acids, phosphorthiolates and N-alkyl-O-phenylcarbamates and their agriculturally acceptable salts and their agriculturally acceptable derivatives such amides, esters, and thioesters, provided they have an acid group.

Examples of preferred safeners C are benoxacor, cloquintocet, cyometrinil, cyprosulfamide, dichlormid, dicyclonon, dietholate, fenchlorazole, fenclorim, flurazole, fluxofenim, furilazole, isoxadifen, mefenpyr, mephenate, naphthalic anhydride, oxabetrinil, 4-(dichloroacetyl)-1-oxa-4-azaspiro[4.5]decane (MON4660, CAS 71526-07-3), 2,2,5-trimethyl-3-(dichloroacetyl)-1,3-oxazolidine (R-29148, CAS 52836-31-4) and N-(2-Methoxybenzoyl)-4-[(methylaminocarbonyl)amino]benzenesulfonamide (CAS 129531-12-0).

Particularly preferred safeners C are the following compounds C.1 to C.17

C.1 benoxacor C.2 cloquintocet C.3 cloquintocet-mexyl C.4 cyprosulfamide C.5 dichlormid C.6 fenchlorazole C.7 fenchlorazole-ethyl C.8 fenclorim C.9 furilazole C.10 isoxadifen C.11 isoxadifen-ethyl C.12 mefenpyr C.13 mefenpyr-diethyl C.14 naphtalic acid anhydride C.15 4-(dichloroacetyl)-1-oxa-4- C.16 2,2,5-trimethyl-3-(dichloro- azaspiro[4.5]decane acetyl)-1,3-oxazolidine C.17 N-(2-Methoxybenzoyl)-4- [(methylaminocarbonyl) amino]benzenesulfonamide

The active compounds B of groups b1) to b15) and the safener compounds C are known herbicides and safeners, see, for example, The Compendium of Pesticide Common Names (http://www.alanwood.net/pesticides/); Farm Chemicals Handbook 2000 volume 86, Meister Publishing Company, 2000; B. Hock, C. Fedtke, R. R. Schmidt, Herbizide [Herbicides], Georg Thieme Verlag, Stuttgart 1995; W. H. Ahrens, Herbicide Handbook, 7th edition, Weed Science Society of America, 1994; and K. K. Hatzios, Herbicide Handbook, Supplement for the 7th edition, Weed Science Society of America, 1998. 2,2,5-Trimethyl-3-(dichloroacetyl)-1,3-oxazolidine [CAS No. 52836-31-4] is also referred to as R-29148. 4-(Dichloroacetyl)-1-oxa-4-azaspiro[4.5]decane [CAS No. 71526-07-3] is also referred to as AD-67 and MON 4660. The assignment of the active compounds to the respective mechanisms of action is based on current knowledge. If several mechanisms of action apply to one active compound, this substance was only assigned to one mechanism of action.

It is generally preferred to use the compounds of the invention in combination with herbicides that are selective for the crop being treated and which complement the spectrum of weeds controlled by these compounds at the application rate employed. It is further generally preferred to apply the compounds of the invention and other complementary herbicides at the same time, either as a combination formulation or as a tank mix.

In another embodiment, the present invention refers to a method for identifying a CESA-inhibiting herbicide by using a wildtype or mutated CESA encoded by a nucleic acid which comprises the nucleotide sequence of SEQ ID NO: 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, or 77, or a variant or derivative thereof.

Said method comprises the steps of:

-   a) generating a transgenic cell or plant comprising a nucleic acid     encoding a wildtype or mutated CESA, wherein the wildtype or mutated     CESA is expressed; -   b) applying a CESA-inhibiting herbicide to the transgenic cell or     plant of a) and to a control cell or plant of the same variety; -   c) determining the growth or the viability of the transgenic cell or     plant and the control cell or plant after application of said     CESA-inhibiting herbicide, and -   d) selecting “CESA-inhibiting herbicides” which confer reduced     growth to the control cell or plant as compared to the growth of the     transgenic cell or plant.

As described above, the present invention teaches compositions and methods for increasing the CESA-inhibiting tolerance of a crop plant or seed as compared to a wild-type variety of the plant or seed. In a preferred embodiment, the CESA-inhibiting tolerance of a crop plant or seed is increased such that the plant or seed can withstand a CESA-inhibiting herbicide application of preferably approximately 1-1000 g ai ha⁻¹, more preferably 1-200 g ai ha⁻¹, even more preferably 5-150 g ai ha⁻¹, and most preferably 10-100 g ai ha⁻¹. As used herein, to “withstand” a CESA-inhibiting herbicide application means that the plant is either not killed or only moderately injured by such application. It will be understood by the person skilled in the art that the application rates may vary, depending on the environmental conditions such as temperature or humidity, and depending on the chosen kind of herbicide (active ingredient ai).

Pre- and/or Post-emergent weed control methods useful in various embodiments hereof utilize about >0.3× application rates of CESA-inhibiting herbicides; in some embodiments, this can be about, for example, >0.3×, >0.4×, >0.5×, >0.6×, >0.7×, >0.8×, >0.9×, or >1× of CESA-inhibiting herbicides. In one embodiment, CESA-inhibiting herbicides-tolerant plants of the present invention have tolerance to a pre- and/or post-emergent application of a CESA-inhibiting herbicides at an amount of about 25 to about 500 g ai/ha. In some embodiments, wherein the CESA-inhibiting herbicides-tolerant plant is a dicot (e.g., soy, cotton), the pre- and/or post-emergent application of the CESA-inhibiting herbicides is at an amount of about 25-250 g ai/ha. In another embodiment, wherein the CESA-inhibiting herbicides-tolerant plant is a monocot (e.g., maize, rice, sorghum), the pre- and/or post-emergent application of the CESA-inhibiting herbicides is at an amount of about 50-500 g ai/ha. In other embodiments, wherein the CESA-inhibiting herbicides-tolerant plant is a Brassica (e.g., canola), the pre- and/or post-emergent application of the CESA-inhibiting herbicides is at an amount of about 25-200 g ai/ha. In pre- and/or post-emergent weed control methods hereof, in some embodiments, the method can utilize CESA-inhibiting herbicides application rates at pre-emergent and/or about 7 to 10 days post-emergent. In another embodiment, the application rate can exceed 8× CESA-inhibiting herbicides; in some embodiments, the rate can be up to 4× CESA-inhibiting herbicides, though more typically it will be about 2.5× or less, or about 2× or less, or about 1× or less.

Furthermore, the present invention provides methods that involve the use of at least one CESA-inhibiting herbicide, optionally in combination with one or more herbicidal compounds B, and, optionally, a safener C, as described in detail supra.

In these methods, the CESA-inhibiting herbicide can be applied by any method known in the art including, but not limited to, seed treatment, soil treatment, and foliar treatment. Prior to application, the CESA-inhibiting herbicide can be converted into the customary formulations, for example solutions, emulsions, suspensions, dusts, powders, pastes and granules. The use form depends on the particular intended purpose; in each case, it should ensure a fine and even distribution of the compound according to the invention.

By providing plants having increased tolerance to CESA-inhibiting herbicide, a wide variety of formulations can be employed for protecting plants from weeds, so as to enhance plant growth and reduce competition for nutrients. A CESA-inhibiting herbicide can be used by itself for pre-emergence, post-emergence, pre-planting, and at-planting control of weeds in areas surrounding the crop plants described herein, or a CESA-inhibiting herbicide formulation can be used that contains other additives. The CESA-inhibiting herbicide can also be used as a seed treatment. Additives found in a CESA-inhibiting herbicide formulation include other herbicides, detergents, adjuvants, spreading agents, sticking agents, stabilizing agents, or the like. The CESA-inhibiting herbicide formulation can be a wet or dry preparation and can include, but is not limited to, flowable powders, emulsifiable concentrates, and liquid concentrates. The CESA-inhibiting herbicide and herbicide formulations can be applied in accordance with conventional methods, for example, by spraying, irrigation, dusting, or the like.

Suitable formulations are described in detail in PCT/EP2009/063387 and PCT/EP2009/063386, which are incorporated herein by reference.

As disclosed herein, the CESA nucleic acids of the invention find use in enhancing the CBI herbicide tolerance of plants that comprise in their genomes a gene encoding a herbicide-tolerant wildtype or mutated CESA protein. Such a gene may be an endogenous gene or a transgene, as described above. Additionally, in certain embodiments, the nucleic acids of the present invention can be stacked with any combination of polynucleotide sequences of interest in order to create plants with a desired phenotype. For example, the nucleic acids of the present invention may be stacked with any other polynucleotides encoding polypeptides having pesticidal and/or insecticidal activity, such as, for example, the Bacillus thuringiensis toxin proteins (described in U.S. Pat. Nos. 5,366,892; 5,747,450; 5,737,514; 5,723,756; 5,593,881; and Geiser et al (1986) Gene 48: 109), 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), Glyphosate acetyl transferase (GAT), cytochrome P450 monooxygenase, phosphinothricin acetyltransferase (PAT), Acetohydroxyacid synthase (AHAS; EC 4.1.3.18, also known as acetolactate synthase or ALS), hydroxyphenyl pyruvate dioxygenase (HPPD), Phytoene desaturase (PD), Protoporphyrinogen oxidase (PPO) and dicamba degrading enzymes as disclosed in WO 02/068607, or phenoxyaceticacid- and phenoxypropionicacid-derivative degrading enzymes as disclosed in WO 2008141154 or WO 2005107437. The combinations generated can also include multiple copies of any one of the polynucleotides of interest.

Consequently, Herbicide-tolerant plants of the invention can be used in conjunction with an herbicide to which they are tolerant. Herbicides can be applied to the plants of the invention using any techniques known to those skilled in the art. Herbicides can be applied at any point in the plant cultivation process. For example, herbicides can be applied pre-planting, at planting, pre-emergence, post-emergence or combinations thereof. Herbicides may be applied to seeds and dried to form a layer on the seeds.

In some embodiments, seeds are treated with a safener, followed by a post-emergent application of a CESA-inhibiting herbicides. In one embodiment, the post-emergent application of the CESA-inhibiting herbicides is about 7 to 10 days following planting of safener-treated seeds. In some embodiments, the safener is cloquintocet, dichlormid, fluxofenim, or combinations thereof.

Methods of Controlling Weeds or Undesired Vegetation

In other aspects, the present invention provides a method for controlling weeds at a locus for growth of a plant or plant part thereof, the method comprising: applying a composition comprising a CESA-inhibiting herbicides to the locus.

In some aspects, the present invention provides a method for controlling weeds at a locus for growth of a plant, the method comprising: applying an herbicide composition comprising CESA-inhibiting herbicides to the locus; wherein said locus is: (a) a locus that contains: a plant or a seed capable of producing said plant; or (b) a locus that is to be after said applying is made to contain the plant or the seed; wherein the plant or the seed comprises in at least some of its cells a polynucleotide operably linked to a promoter operable in plant cells, the promoter capable of expressing a wildtype or mutated CESA polypeptide encoded by the polynucleotide, the expression of the wildtype or mutated CESA polypeptide conferring to the plant tolerance to CESA-inhibiting herbicides.

Herbicide compositions hereof can be applied, e.g., as foliar treatments, soil treatments, seed treatments, or soil drenches. Application can be made, e.g., by spraying, dusting, broadcasting, or any other mode known useful in the art.

In one embodiment, herbicides can be used to control the growth of weeds that may be found growing in the vicinity of the herbicide-tolerant plants invention. In embodiments of this type, an herbicide can be applied to a plot in which herbicide-tolerant plants of the invention are growing in vicinity to weeds. An herbicide to which the herbicide-tolerant plant of the invention is tolerant can then be applied to the plot at a concentration sufficient to kill or inhibit the growth of the weed. Concentrations of herbicide sufficient to kill or inhibit the growth of weeds are known in the art and are disclosed above.

In other embodiments, the present invention provides a method for controlling weeds in the vicinity of a CESA-inhibiting herbicides-tolerant plant of the invention. The method comprises applying an effective amount of a CESA-inhibiting herbicides to the weeds and to the herbicide-tolerant plant, wherein the plant has increased tolerance to CESA-inhibiting herbicide when compared to a wild-type plant. In some embodiments, the CESA-inhibiting herbicides-tolerant plants of the invention are preferably crop plants, including, but not limited to, sunflower, alfalfa, Brassica sp., soybean, cotton, safflower, peanut, tobacco, tomato, potato, wheat, rice, maize, sorghum, barley, rye, millet, and sorghum.

In other aspects, herbicide(s) (e.g., CESA-inhibiting herbicides) can also be used as a seed treatment. In some embodiments, an effective concentration or an effective amount of herbicide(s), or a composition comprising an effective concentration or an effective amount of herbicide(s) can be applied directly to the seeds prior to or during the sowing of the seeds. Seed Treatment formulations may additionally comprise binders and optionally colorants.

Binders can be added to improve the adhesion of the active materials on the seeds after treatment. In one embodiments, suitable binders are block copolymers EO/PO surfactants but also polyvinylalcoholsl, polyvinylpyrrolidones, polyacrylates, polymethacrylates, polybutenes, polyisobutylenes, polystyrene, polyethyleneamines, polyethyleneamides, polyethyleneimines (Lupasol®, Polymin®), polyethers, polyurethans, polyvinylacetate, tylose and copolymers derived from these polymers. Optionally, also colorants can be included in the formulation. Suitable colorants or dyes for seed treatment formulations are Rhodamin B, C.I. Pigment Red 112, C.I. Solvent Red 1, pigment blue 15:4, pigment blue 15:3, pigment blue 15:2, pigment blue 15:1, pigment blue 80, pigment yellow 1, pigment yellow 13, pigment red 112, pigment red 48:2, pigment red 48:1, pigment red 57:1, pigment red 53:1, pigment orange 43, pigment orange 34, pigment orange 5, pigment green 36, pigment green 7, pigment white 6, pigment brown 25, basic violet 10, basic violet 49, acid red 51, acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108.

The term seed treatment comprises all suitable seed treatment techniques known in the art, such as seed dressing, seed coating, seed dusting, seed soaking, and seed pelleting. In one embodiment, the present invention provides a method of treating soil by the application, in particular into the seed drill: either of a granular formulation containing the CESA-inhibiting herbicides as a composition/formulation (e.g., a granular formulation), with optionally one or more solid or liquid, agriculturally acceptable carriers and/or optionally with one or more agriculturally acceptable surfactants. This method is advantageously employed, for example, in seedbeds of cereals, maize, cotton, and sunflower.

The present invention also comprises seeds coated with or containing with a seed treatment formulation comprising CESA-inhibiting herbicides and at least one other herbicide such as, e.g., an AHAS-inhibitor selected from the group consisting of amidosulfuron, azimsulfuron, bensulfuron, chlorimuron, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron, ethoxysulfuron, flazasulfuron, flupyrsulfuron, foramsulfuron, halosulfuron, imazosulfuron, iodosulfuron, mesosulfuron, metsulfuron, nicosulfuron, oxasulfuron, primisulfuron, prosulfuron, pyrazosulfuron, rimsulfuron, sulfometuron, sulfosulfuron, thifensulfuron, triasulfuron, tribenuron, trifloxysulfuron, triflusulfuron, tritosulfuron, imazamethabenz, imazamox, imazapic, imazapyr, imazaquin, imazethapyr, cloransulam, diclosulam, florasulam, flumetsulam, metosulam, penoxsulam, bispyribac, pyriminobac, propoxycarbazone, flucarbazone, pyribenzoxim, pyriftalid and pyrithiobac.

The term “coated with and/or containing” generally signifies that the active ingredient is for the most part on the surface of the propagation product at the time of application, although a greater or lesser part of the ingredient may penetrate into the propagation product, depending on the method of application. When the said propagation product is (re)planted, it may absorb the active ingredient.

In some embodiments, the seed treatment application with CESA-inhibiting herbicides or with a formulation comprising the CESA-inhibiting herbicides is carried out by spraying or dusting the seeds before sowing of the plants and before emergence of the plants.

In other embodiments, in the treatment of seeds, the corresponding formulations are applied by treating the seeds with an effective amount of CESA-inhibiting herbicides or a formulation comprising the CESA-inhibiting herbicides.

In other aspects, the present invention provides a method for combating undesired vegetation or controlling weeds comprising contacting the seeds of the CESA-inhibiting herbicides-tolerant plants of the present invention before sowing and/or after pregermination with CESA-inhibiting herbicides. The method can further comprise sowing the seeds, for example, in soil in a field or in a potting medium in greenhouse. The method finds particular use in combating undesired vegetation or controlling weeds in the immediate vicinity of the seed. The control of undesired vegetation is understood as the killing of weeds and/or otherwise retarding or inhibiting the normal growth of the weeds. Weeds, in the broadest sense, are understood as meaning all those plants which grow in locations where they are undesired.

The weeds of the present invention include, for example, dicotyledonous and monocotyledonous weeds. Dicotyledonous weeds include, but are not limited to, weeds of the genera: Sinapis, Lepiclium, Galium, Stellaria, Matricaria, Anthemis, Galinsoga, Chenopodium, Urtica, Senecio, Amaranthus, Portulaca, Xanthium, Convolvulus, Ipomoea, Polygonum, Sesbania, Ambrosia, Cirsium, Carduus, Sonchus, Solarium, Rorippa, Rotala, Lindernia, Lamium, Veronica, Abutilon, Emex, Datura, Viola, Galeopsis, Papaver, Centaurea, Trifolium, Ranunculus, and Taraxacum. Monocotyledonous weeds include, but are not limited to, weeds of the genera: Echinochloa, Setaria, Panicum, Digitaria, Phleum, Poa, Festuca, Eleusine, Brachiaria, Lolium, Bromus, Avena, Cyperus, Sorghum, Agropyron, Cynodon, Monochoria, Fimbristyslis, Sagittaria, Eleocharis, Scirpus, Paspalum, Ischaemum, Sphenoclea, Dactyloctenium, Agrostis, Alopecurus, and Apera.

In addition, the weeds of the present invention can include, for example, crop plants that are growing in an undesired location. For example, a volunteer maize plant that is in a field that predominantly comprises soybean plants can be considered a weed, if the maize plant is undesired in the field of soybean plants.

In other embodiments, in the treatment of seeds, the corresponding formulations are applied by treating the seeds with an effective amount of CESA-inhibiting herbicides or a formulation comprising the CESA-inhibiting herbicides.

In still further aspects, treatment of loci, plants, plant parts, or seeds of the present invention comprises application of an agronomically acceptable composition that does not contain an A.I. In one embodiment, the treatment comprises application of an agronomically acceptable composition that does not contain a CESA-inhibiting herbicides A.I. In some embodiments, the treatment comprises application of an agronomically acceptable composition that does not contain a CESA-inhibiting herbicides A.L, wherein the composition comprises one or more of agronomically-acceptable carriers, diluents, excipients, plant growth regulators, and the like. In other embodiments, the treatment comprises application of an agronomically acceptable composition that does not contain a CESA-inhibiting herbicides A.I., wherein the composition comprises an adjuvant. In one embodiment, the adjuvant is a surfactant, a spreader, a sticker, a penetrant, a drift-control agent, a crop oil, an emulsifier, a compatibility agent, or combinations thereof.

It should also be understood that the foregoing relates to preferred embodiments of the present invention and that numerous changes may be made therein without departing from the scope of the invention. The invention is further illustrated by the following examples, which are not to be construed in any way as imposing limitations upon the scope thereof. On the contrary, it is to be clearly understood that resort may be had to various other embodiments, modifications, and equivalents thereof, which, after reading the description herein, may suggest themselves to those skilled in the art without departing from the spirit of the present invention and/or the scope of the appended claims.

EXAMPLES Example 1: Identification of Cellulose Biosynthesis Inhibitor (CBI; CESA Inhibitor) Resistant Plants

For selection of cellulose biosynthesis inhibitor resistant plants EMS mutagenized seed populations of Arabidopsis thaliana are used. EMS mutagenized seed populations are either bought from Lehle Seeds (1102 South Industrial Blvd. Suite D, Round Rock, Tex. USA) or are generated as described elsewhere (Heim D R, et. al. (1989) Plant Physiol. 90: 146-150). Causative mutations in CESA wildtype sequences (e.g. SEQ ID NO: 1 or 3) are identified as described by Scheible W R et. al. (2001, Proc. Natl. Acad. of Sci. 98: 10079-10084), McCourt et. al. (WO2013/142968) or with the use of next generation sequencing methods as described by Austin R S, et. al. (2011) Plant Journal 67: 715-725.

Selected Arabidopsis thaliana lines were assayed for improved resistance to azines like 6-cyclopentyl-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine; 6-(1-fluoro-1-methyl-ethyl)-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine; 6-cyclohexyl-N2-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine; 6-(2,6-difluorophenyl)-N2-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine-2,4-diamine; N2-(4-chloro-3,5,6-trifluoro-2-pyridyl)-6-(1-fluoro-1-methyl-ethyl)-1,3,5-triazine-2,4-diamine in 48-well plates. Therefore, M2 or M3 seeds are surface sterilized by stirring for 5 min in ethanol+water (70+30 by volume), rinsing one time with ethanol+water (70+30 by volume) and two times with sterile, deionized water. The seeds are resuspended in 0.1% agar dissolved in water (w/v) Four to five seeds per well are plated on solid nutrient medium consisting of half-strength murashige skoog nutrient solution, pH 5.8 (Murashige and Skoog (1962) Physiologia Plantarum 15: 473-497). Compounds are dissolved in dimethylsulfoxid (DMSO) and added to the medium prior solidification (final DMSO concentration 0.1%). Multi well plates are incubated in a growth chamber at 22° C., 75% relative humidity and 110 μmol Phot*m⁻²*s⁻¹ with 14:10 h light:dark photoperiod. Growth inhibition is evaluated seven to ten days after seeding in comparison to wild type plants. Tolerance factors are calculated based on IC50 values of growth inhibition of transformed versus non-transformed Arabidopsis plants (Table 3).

TABLE 3 Tolerance of cellulose synthase mutant lines compared to wildtype plants. Relative tolerance rates of mutant Arabidopsis lines compared to a wildtype Arabidopsis plants (wildtype = 1.0), treated with various cellulose biosynthesis inhibitors. Growth inhibition is evaluated seven to ten days after seeding in comparison to wild type plants. 6-(1-fluoro- 6-(2,6- N2-(4-chloro-3,5,6- 6-cyclopentyl- 1-methyl-ethyl)- 6-cyclohexyl- difluorophenyl)- trifluoro-2-pyridyl)- N4-(2,3,4,5,6- N4-(2,3,4,5,6- N2-(2,3,4,5,6- N2-(2,3,5,6- 6-(1-fluoro-1- pentafluorophenyl)- pentafluorophenyl)- pentafluorophenyl)- tetrafluorophenyl)- methyl-ethyl)- 1,3,5-triazine-2,4- 1,3,5-triazine-2,4- 1,3,5-triazine-2,4- 1,3,5-triazine-2,4- 1,3,5-triazine- Mutant diamine diamine diamine diamine 2,4-diamine wildtype 1 1 1 1 1 Cesa3_S1040L 56 2 10 13 4 Cesa3_S1037F 75 4 1 1 10 Cesa3_S983F 75 1 1 1 4 Cesa1_G1013R 25 4 4 13 10 Cesa1_P1010L 3 1 1 2 1 Cesa1_G1009D 3 2 1 1 1

Additionally, M2 or M3 Arabidopsis plants are tested for improved tolerance to cellulose biosynthesis-inhibiting herbicides in greenhouse studies (e. g. with the following cellulose biosynthesis-inhibiting herbicides: 6-cyclopentyl-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine; 6-(1-fluoro-1-methyl-ethyl)-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine; N4-(4-chloro-3,5,6-trifluoro-2-pyridyl)-6-(1-fluoro-1-methyl-ethyl)-1,3,5-triazine-2,4-diamine; 6-(2,6-difluorophenyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine-2,4-diamine; N4-(4-bromo-2,3,5,6-tetrafluoro-phenyl)-6-(1-fluoro-1-methyl-ethyl)-1,3,5-triazine-2,4-diamine.

TABLE 4 Phytotox values of cellulose synthase mutant lines to various cellulose biosynthesis inhibitors when treated pre-emergent. Shown are phytotox values on a scale from 0-100, were 100 is 100% damage. A. thaliana Cesa3_ Cesa3_ Cesa3_ Cesa1_ Cesa1_ Cesa1_ WT L. S1040L, S1037F, S983F, G1013R, P1010L, G1009D, compound g ai/ha erecta fpx1-1 fpx1-2 fpx1-3 fpx2-1 fpx2-2 fpx2-3 6-cyclopentyl-N4- 15.63 100 40 5 40 15 98 88 (2,3,4,5,6- 3.91 99 25 15 20 25 63 58 pentafluorophenyl)-1,3,5- 0.98 80 20 23 5 15 33 25 triazine-2,4-diamine 0.25 38 5 0 0 5 0 5 1-(m-tolyl)-5-phenyl- 250 100 25 68 30 15 35 45 1,2,4-triazole- 62.5 100 0 23 5 20 13 30 3-carboxamide 15.63 63 30 30 8 15 20 18 3.91 0 38 0 0 5 0 0 6-(1-fluoro-1-methyl- 3.91 100 98 80 98 97 100 100 ethyl)-N4-(2,3,4,5,6- 0.98 100 75 30 75 63 99 97 pentafluorophenyl)-1,3,5- 0.25 78 65 20 45 65 50 78 triazine-2,4-diamine 0.0625 38 20 10 78 13 43 65 N4-(4-chloro-3,5,6- 3.91 99 53 25 13 80 98 99 trifluoro-2-pyridyl)- 0.98 83 78 25 48 70 78 93 6-(1-fluoro- 0.25 60 15 15 55 50 25 43 methyl-ethyl)-1,3,5- 0.0625 45 13 5 23 40 50 30 triazine-2,4-diamine 6-(2,6-difluorophenyl)- 62.5 100 28 99 33 40 98 95 N4-(2,3,5,6- 15.63 90 25 73 43 28 65 18 tetrafluorophenyl)-1,3,5- 3.91 70 8 65 35 35 35 38 triazine-2,4-diamine 0.98 30 13 28 18 15 48 65 N4-(4-bromo-2,3,5,6- 15.63 100 93 30 45 73 99 99 tetrafluoro-phenyl)-6-(1- 3.91 97 63 33 25 35 88 68 fluoro-1-methyl-ethyl)- 0.98 35 5 18 23 10 25 35 1,3,5-triazine-2,4-diamine 0.25 8 13 28 35 10 25 50

TABLE 5 Phytotox values of cellulose synthase mutant lines to various cellulose biosynthesis inhibitors when treated post-emergent, 6 weeks after sowing. Shown are phytotox values on a scale from 0-100, were 100 is 100% damage. A. thaliana Cesa3_ Cesa3_ Cesa3_ Cesa1_ Cesa1_ Cesa1_ WT S1040L, S1037F, S983F, G1013R, P1010L, G1009D, compound g ai/ha L. erecta fpx1-1 fpx1-2 fpx1-3 fpx2-1 fpx2-2 fpx2-3 6-cyclopentyl-N4- 63 98 68 78 73 80 93 75 (2,3,4,5,6- 31.5 78 35 70 55 65 78 73 pentafluorophenyl)- 15.75 80 5 58 65 55 70 63 1,3,5-triazine-2,4- 7.88 68 0 55 25 38 63 45 diamine 1-(m-tolyl)-5- phenyl-1,2,4- 250 95 55 68 53 40 48 45 triazole-3- 125 83 23 50 23 33 15 33 carboxamide 62.5 78 0 30 28 23 15 23

Example 2: Identification of Homologue Cellulose Synthase Isoforms in Crop Plants

To identify homologue cellulose synthase genes from soy, corn and rice, BLAST searches using the protein sequences of Arabidopsis thaliana cellulose synthase isoforms were performed (Altschul et al. (1990) J Mol Biol 215: 403-10). Cellulose synthase protein encoding genes from corn, soy and rice were analyzed regarding their phylogenetic relationship by the R software library phangorn (Schliep K P. (2011) Bioinformatics 27: 592-593). Bootstrap analyses to statistically confirm monophyletic groups were calculated. In addition genes were classified by their expression level and expression pattern (Hruz T, et. al. (2008) Adv. in Bioinformatics 2008: 1-5) (FIGS. 1-3) and selected for plant transformation.

Example 3: Tissue Culture Conditions

An in vitro tissue culture mutagenesis assay is developed to isolate and characterize plant tissue (e.g., maize, rice) that is tolerant to cellulose synthase inhibiting herbicides, (e. g. 6-cyclopentyl-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine, and photosynthesis inhibitor diuron as negative control). The assay utilizes the somaclonal variation that is found in in vitro tissue culture. Spontaneous mutations derived from somaclonal variation can be enhanced by chemical mutagenesis (e. g. ethylmethane sulfonate; ethylethane sulfonate, N-nitroso-N-ethyl urea, ethylnitrosourea, nitrose acid, bromouracil, 2.-aminopurine, 5-fluorodeoxyuridine, hydroxylamine, N-methyl-N′-nitro-N-nitrosoguanidine) and subsequent selection in a stepwise manner, on increasing concentrations of herbicide.

The present invention provides tissue culture conditions for encouraging growth of friable, embryogenic maize or rice callus that is regenerable. Calli are initiated from 4 different maize or rice cultivars encompassing Zea mays and Japonica (Taipei 309, Nipponbare, Koshihikari) and Indica (Indica 1) varieties, respectively. Seeds are surface sterilized in 70% ethanol for approximately 1 min followed by 20% commercial Clorox bleach for 20 minutes. Seeds are rinsed with sterile water and plated on callus induction media. Various callus induction media are tested. The ingredient lists for the media tested are presented in Table 6.

TABLE 6 Ingredient Supplier R001M R025M R026M R327M R008M MS711R B5 Vitamins Sigma 1.0 X MS salts Sigma 1.0 X 1.0 X 1.0 X 1.0 X MS Vitamins Sigma 1.0 X 1.0 X N6 salts Phytotech 4.0 g/L 4.0 g/L N6 vitamins Phytotech 1.0 X 1.0 X L-Proline Sigma 2.9 g/L 0.5 g/L 1.2 g/L Casamino Acids BD 0.3 g/L 0.3 g/L 2 g/L Casein Sigma 1.0 g/L Hydrolysate L-Asp Phytotech 150 mg/L Monohydrate Nicotinic Acid Sigma 0.5 mg/L Pyridoxine HCl Sigma 0.5 mg/L Thiamine HCl Sigma 1.0 mg/L Myo-inositol Sigma 100 mg/L MES Sigma 500 mg/L 500 mg/L 500 mg/L 500 mg/L 500 mg/L 500 mg/L Maltose VWR 30 g/L 30 g/L 30 g/L 30 g/L Sorbitol Duchefa 30 g/L Sucrose VWR 10 g/L 30 g/L NAA Duchefa 50 μg/L 2,4-D Sigma 2.0 mg/L 1.0 mg/L MgCl₂•6H₂O VWR 750 mg/L →pH 5.8 5.8 5.8 5.8 5.8 5.7 Gelrite Duchefa 4.0 g/L 2.5 g/L Agarose Type1 Sigma 7.0 g/L 10 g/L 10 g/L →Autoclave 15 min 15 min 15 min 15 min 15 min 20 min Kinetin Sigma 2.0 mg/L 2.0 mg/L NAA Duchefa 1.0 mg/L 1.0 mg/L ABA Sigma 5.0 mg/L Cefotaxime Duchefa 0.1 g/L 0.1 g/L 0.1 g/L Vancomycin Duchefa 0.1 g/L 0.1 g/L 0.1 g/L G418 Disulfate Sigma 20 mg/L 20 mg/L 20 mg/L

R001M callus induction media was selected after testing numerous variations. Cultures were kept in the dark at 30° C. Embryogenic callus was subcultured to fresh media after 10-14 days.

Example 4: Selection of Herbicide-Tolerant Calli

Once tissue culture conditions were determined, further establishment of selection conditions are established through the analysis of tissue survival in kill curves with azine herbicides e. g. like 6-cyclopentyl-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine, and photosynthesis inhibitor diuron as negative control. Careful consideration of accumulation of the herbicide in the tissue, as well as its persistence and stability in the cells and the culture media are performed. Through these experiments, a sub-lethal dose is established for the initial selection of mutated material. After the establishment of the starting dose of azine herbicides like e. g. 6-cyclopentyl-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine, and photosynthesis inhibitor diuron as negative control in selection media, the tissues were selected in a step-wise fashion by increasing the concentration of the cellulose synthase inhibitor with each transfer until cells are recovered that grew vigorously in the presence of toxic doses. The resulting calli are further subcultured every 3-4 weeks to R001M with selective agent. Over 26,000 calli are subjected to selection for 4-5 subcultures until the selective pressure is above toxic levels as determined by kill curves and observations of continued culture. Alternatively, liquid cultures initiated from calli in MS711R with slow shaking and weekly subcultures. Once liquid cultures are established, selection agent is added directly to the flask at each subculture. Following 2-4 rounds of liquid selection, cultures are transferred to filters on solid R001M media for further growth.

Example 5: Regeneration of Plants

Tolerant tissue is regenerated and characterized molecularly for cellulose synthase gene sequences mutations. In addition, genes involved directly and/or indirectly in cell wall biosynthesis and/or metabolism pathways are also sequenced to characterize mutations. Finally, enzymes that change the fate (e.g. metabolism, translocation, transportation) are also sequenced to characterize mutations. Following herbicide selection, calli are regenerated using a media regime of R025M for 10-14 days, R026M for ca. 2 weeks, R327M until well formed shoots were developed, and R008S until shoots are well rooted for transfer to the greenhouse. Regeneration was carried out in the light. No selection agent is included during regeneration. Once strong roots are established, M0 regenerants are transplant to the greenhouse in square or round pots. Transplants are maintained under a clear plastic cup until they were adapted to greenhouse conditions. The greenhouse was set to a day/night cycle of 27° C./21° C. (80° F./70° F.) with 600 W high pressure sodium lights supplementing light to maintain a 14 hour day length. Plants are watered according to need, depending in the weather and fertilized daily.

Example 6: Sequence Analysis

Leaf tissue is collected from clonal plants separated for transplanting and analyzed as individuals. Genomic DNA is extracted using a Wizard® 96 Magnetic DNA Plant System kit (Promega, U.S. Pat. Nos. 6,027,945 & 6,368,800) as directed by the manufacturer. Isolated DNA is PCR amplified using the appropriate forward and reverse primer.

PCR amplification is performed using Hotstar Taq DNA Polymerase (Qiagen) using touchdown thermocycling program as follows: 96° C. for 15 min, followed by 35 cycles (96° C., 30 sec; 58° C.-0.2° C. per cycle, 30 sec; 72° C., 3 min and 30 sec), 10 min at 72° C. PCR products were verified for concentration and fragment size via agarose gel electrophoresis. Dephosphorylated PCR products are analyzed by direct sequence using the PCR primers (DNA Landmarks). Chromatogram trace files (.scf) are analyzed for mutation relative to the wild-type gene using Vector NTI Advance 10™ (Invitrogen). Based on sequence information, mutations are identified in several individuals. Sequence analysis is performed on the representative chromatograms and corresponding AlignX alignment with default settings and edited to call secondary peaks.

Example 7: Engineering Azine-Tolerant Arabidopsis Plants Having Wildtype or Mutated Cellulose Synthase Sequences

For transformation of Arabidopsis thaliana, wildtype or mutated cellulose synthase sequences based on one of the following sequences SEQ ID NO: 72, 73, 74, 75, 76, or 77, are cloned with standard cloning techniques as described in Sambrook et al. (Molecular cloning (2001) Cold Spring Harbor Laboratory Press) in a binary vector containing resistance marker gene cassette (AHAS) and mutated cellulose synthase sequence (marked as GOI) in between ubiquitin promoter (PcUbi) and nopaline synthase terminator (NOS) sequence. Binary plasmids are introduced to Agrobacterium tumefaciens for plant transformation. Arabidopsis thaliana are transformed with wildtype or mutated cellulose synthase sequences by floral dip method as described by McElver and Singh (WO 2008/124495). Transgenic Arabidopsis plants are subjected to TaqMan analysis for analysis of the number of integration loci.

Transgenic Arabidopsis thaliana plants are assayed for improved tolerance to azine herbicides like e. g. 6-cyclopentyl-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine in 48-well plates. Therefore, T2 seeds are surface sterilized by stirring for 5 min in ethanol+water (70+30 by volume), rinsing one time with ethanol+water (70+30 by volume) and two times with sterile, deionized water. The seeds are resuspended in 0.1% agar dissolved in water (w/v) Four to five seeds per well are plated on solid nutrient medium consisting of half-strength murashige skoog nutrient solution, pH 5.8 (Murashige and Skoog (1962) Physiologia Plantarum 15: 473-497). Compounds are dissolved in dimethylsulfoxid (DMSO) and added to the medium prior solidification (final DMSO concentration 0.1%). Multi well plates are incubated in a growth chamber at 22° C., 75% relative humidity and 110 μmol Phot*m⁻²*s⁻¹ with 14:10 h light:dark photoperiod. Growth inhibition is evaluated seven to ten days after seeding in comparison to wild type plants. Tolerance factors are calculated based on IC50 values of growth inhibition of transformed versus non-transformed Arabidopsis plants.

TABLE 7 Relative tolerance rates of transgenic Arabidopsis plants as compared to a non-transgenic Arabidopsis plant (non-transgenic = 1.0), treated with various cellulose biosynthesis inhibitors. Growth inhibition is evaluated seven to ten days after seeding in comparison to wild type plants. construct AtCESA1 wt AtCESA1_G1013R AtCESA3_wt AtCESA3_S1040L AtCESA3_S1037F SEQ ID NO 1 1 3 3   3 substitution — G1013R — S1040L S1037F 1-(m-tolyl)-5-phenyl- 3 33  4 2 1333 1,2,4-triazole-3-carboxamide 6-cyclopentyl-N4-(2,3,4,5,6- 1 3 1 1  17 pentafluorophenyl)- 1,3,5-triazine-2,4-diamine

Additionally, transgenic T2 Arabidopsis plants are tested for improved tolerance to CESA-inhibiting herbicides in greenhouse studies with azine compounds like e. g. 6-cyclopentyl-N4-(2,3,4,5,6-pentafluorophenyl)-1,3,5-triazine-2,4-diamine.

Example 8: Soybean Transformation and Cellulose Biosynthesis Inhibitor Tolerance Testing

Binary vectors are generated as described in EXAMPLE 7. Soybean cv Jake are transformed as previously described by Siminszky et al., Phytochem Rev. 5:445-458 (2006). After regeneration, transformants are transplanted to soil in small pots, placed in growth chambers (16 hr day/8 hr night; 25° C. day/23° C. night; 65% relative humidity; 130-150 microE m−2 s−1) and subsequently tested for the presence of the T-DNA via Taqman analysis. After a few weeks, healthy, transgenic positive, single copy events are transplanted to larger pots and allowed to grow in the growth chamber. An optimal shoot for cutting is about 3-4 inches tall, with at least two nodes present. Each cutting is taken from the original transformant (mother plant) and dipped into rooting hormone powder (indole-3-butyric acid, IBA). The cutting is then placed in oasis wedges inside a bio-dome. The mother plant is taken to maturity in the greenhouse and harvested for seed. Wild type cuttings are also taken simultaneously to serve as negative controls. The cuttings are kept in the bio-dome for 5-7 days. 3-4 days after transfer to oasis wedges, the shoots are treated via nutrient solution with the herbicide. Typical phytotox symptoms, like club shaped root, are evaluated 3-4 days after treatment. Less or no injury of transgenic plants compared to wildtype plants are interpreted as herbicide tolerance.

Example 9: Engineering Cellulose Biosynthesis Inhibitor Tolerant Corn or Rice Plants Having Mutated Cellulose Synthase Sequences

Immature embryos can be transformed according to the procedure outlined in Peng et al. (WO2006/136596). Plants are tested for the presence of the T-DNA by Taqman an analysis with the target being the nos terminator which is present in all constructs. Healthy looking plants are sent to the greenhouse for hardening and subsequent spray testing. The plants are individually transplanted into MetroMix 360 soil in 4″ pots. Once in the greenhouse (day/night cycle of 27° C./21° C. with 14 hour day length supported by 600 W high pressure sodium lights), they are allowed to grow for 14 days. Transformation of Oryza sativa (rice) are done by protoplast transformation as described by Peng et al. (U.S. Pat. No. 6,653,529). Transgenic corn and rice plants are cultivated to T1 seeds for herbicide tolerance testing.

Example 10: Demonstration of Herbicide Tolerance

T0 or T1 transgenic plant of soybean, corn and rice containing cellulose synthase sequences or mutated gene variants thereof are tested for improved tolerance to herbicides in greenhouse studies and mini-plot studies with azine herbicides. For the pre-emergence treatment, the herbicides are applied directly after sowing by means of finely distributing nozzles. The containers are irrigated gently to promote germination and growth and subsequently covered with transparent plastic hoods until the plants have rooted. This cover causes uniform germination of the test plants, unless this has been impaired by the herbicides. For post emergence treatment, the test plants are first grown to a height of 3 to 15 cm, depending on the plant habit, and only then treated with the herbicides. For this purpose, the test plants are either sown directly, and grown in the same containers or they are first grown separately and transplanted into the test containers a few days prior to treatment.

Herbicide injury evaluations are taken at 2 and 3 weeks after treatment. Plant injury is rated on a scale of 0% to 100%, 0% being no injury and 100% being complete death.

The following gives a definition of the injury scores measured above:

Score Description of injury 0 No Injury 1 Minimal injury, only a few patches of leaf injury or chlorosis. 2 Minimal injury with slightly stronger chlorosis. Overall growth points remain undamaged. 3 Slightly stronger injury on secondary leaf tissue, but primary leaf and growth points are still undamaged. 4 Overall plant morphology is slightly different, some chlorosis and necrosis in secondary growth points and leaf tissue. Stems are intact. Regrowth is highly probable within 1 week. 5 Overall plant morphology is clearly different, some chlorosis and necrosis on a few leaves and growth points, but primary growth point is intact. Stem tissue is still green. Regrowth is highly probably within 1 week. 6 Strong injury can be seen on the new leaflet growth. Plant has a high probability to survive only through regrowth at different growth points. Most of the leaves are chlorotic/ necrotic but stem tissue is still green. May have regrowth but with noticeable injured appearance. 7 Most of the active growth points are necrotic. There may be a single growth point that could survive and may be partially chlorotic or green and partially necrotic. Two leaves may still be chlorotic with some green; the rest of the plant including stem is necrotic. 8 Plant will likely die, and all growth points are necrotic. One leaf may still be chlorotic with some green. The remainder of the plant is necrotic. 9 Plant is dead. * Not tested 

The invention claimed is:
 1. A plant or plant part comprising a polynucleotide encoding a mutated cellulose synthase (CESA) polypeptide, wherein the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, wherein the mutated CESA polypeptide comprises on or more of the following motifs: i) Motif 1a: (SEQ ID NO: 78) [V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G] [S/N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[F/M/L]G [K/R][L/V][F/L]F,

wherein the amino acid at position 5, 16, and/or 17, within said motif is substituted by any other amino acid; ii) Motif 2a: (SEQ ID NO: 81) [V/L/I]W[S/A][V/A/I]LL[A/S]S[I/F/V][F/L][S/T] [L/V][L/M/V/I]WV[R/K][I/V][N/D]PF,

wherein the amino acid at position 8 and/or 11 within said motif is substituted by any other amino acid.
 2. The plant or plant part of claim 1, wherein the mutated CESA polypeptide is a functional variant having, over the full-length of the variant, at least about 60% amino acid sequence identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, or
 65. 3. The plant or plant part of claim 1, wherein the mutated CESA is to a CESA polypeptide comprising the sequence of SEQ ID NO: 1, an orthologue, paralogue, or homologue thereof, wherein the amino acid sequence differs from the wildtype amino acid sequence at one or more positions corresponding to positions 998, 1009, 1010, 1013, 1052, 1055 of SEQ ID NO:
 1. 4. The plant or plant part of claim 1, wherein the plant part is a seed.
 5. The plant or plant part of claim 1, wherein the plant is a plant cell.
 6. A plant product prepared from the plant or plant part of claim
 1. 7. A method of producing a plant having tolerance to CESA-inhibiting herbicide, the method comprising regenerating a plant from the plant cell of claim
 5. 8. The plant or plant part of claim 1, wherein the plant or plant part further exhibits a second or third herbicide-tolerant trait.
 9. A method for producing a plant product from the plant of claim 1, the method comprising processing the plant or a plant part thereof to obtain the plant product.
 10. The method of claim 9, wherein the plant product is fodder, seed meal, oil, or seed-treatment-coated seeds.
 11. The plant product of claim 6, wherein the product is fodder, seed meal, oil, or seed-treatment-coated seed. 